Team:Aachen/Project/Measurement Device

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* incubate the sensing cells and the sampling chip
* incubate the sensing cells and the sampling chip
* prevent escape of potentially sampled pathogens and our genetically engineered cells
* prevent escape of potentially sampled pathogens and our genetically engineered cells
-
* illuminate the chip with the right excitation wavelength for GFP
+
* illuminate the chip with the right excitation wavelength for GFP or iLOV
-
* take a photo of the chips
+
* take photos and timeseries of the chips
* analyze the fluorescence signal
* analyze the fluorescence signal
* give feedback to the user about the presence or absence of ''P. aeruginosa''
* give feedback to the user about the presence or absence of ''P. aeruginosa''

Revision as of 21:19, 13 October 2014

WatsOn

Part of the biosensor system Cellock Holmes are our agar chips. They contain our engineered sensing cells that fluoresce in the presence of the pathogen Pseudomonas aeruginosa. However, bacteria are small and the fluorescent signal cannot be seen with the naked eye. Thus the central question "What's on the chip?" remains.
To answer this question we present our measurement device WatsOn.

WatsOn fulfills several tasks in our system:

  • incubate the sensing cells and the sampling chip
  • prevent escape of potentially sampled pathogens and our genetically engineered cells
  • illuminate the chip with the right excitation wavelength for GFP or iLOV
  • take photos and timeseries of the chips
  • analyze the fluorescence signal
  • give feedback to the user about the presence or absence of P. aeruginosa


Measurarty

Measurements

Software Evaluation

Secret Ingredients

Arduino Sketchbook (.ino)

Build your own WatsOn

If you want to create your own WatsOn at first check the following list of necessary devices.

Table 1: Needed number of pieces, components and costs for building your own measurement device

number of pieces components costs [$]
1 [http://www.prolighting.de/Zubehoer/Farbfilter/Lee-Filter_HT/Lee-Filters_Musterheft_Designer_Edition_i174_3965_0.htm filter slides] (medium yellow 010, Double G&T 514)5.17
1 [http://www.dx.com/p/uno-r3-development-board-microcontroller-mega328p-atmega16u2-compat-for-arduino-blue-black-215600 arduino UNO R3]11.65
1 [http://www.dx.com/p/arduino-2-channel-relay-shield-module-red-144140 2-channel relay shield]3.46
40jumper-wire cable2.99
1 [http://www.dx.com/p/2-54mm-1x40-pin-breakaway-straight-male-header-10-piece-pack-144191 40er male header (10-Piece Pack)]2.72
1 [http://www.dx.com/p/jtron-2-54mm-40-pin-single-row-seat-single-row-female-header-black-10-pcs-278953 40er female header (10-Piece Pack)]2.60
1 [http://www.dx.com/p/prototype-universal-printed-circuit-board-breadboard-brown-5-piece-pack-130926 circuit board]2.99
1 [http://www.newark.com/pro-signal/rp006/audio-video-cable-hdmi-1m-black/dp/96T7446 HDMI cable]1.87
1 [http://www.dx.com/p/hd-053-high-speed-usb-2-0-7-port-hub-black-174817 7 port USB hub]6.71
1[http://www.dx.com/p/dx-original-ultra-mini-usb-2-0-802-11n-b-g-150mbps-wi-fi-wlan-wireless-network-adapter-black-252716 WLAN USB stick]5.35
1USB mouse and keyboard12.50
1 [http://corporate.evonik.com/en/products/pages/default.aspx case acrylic glass XT 6mm~0.52]50.65
1spray paint for acrylic glass6.54
1 [http://www.newark.com/raspberry-pi/raspberry-modb-512m/raspberry-pi-model-b-board/dp/68X0155 Raspberry Pi model B board]35.00
1[http://www.newark.com/raspberry-pi/rpi-camera-board/add-on-brd-camera-module-raspberry/dp/69W0689 Raspberry Pi camera module]25.00
1[http://www.pollin.de/shop/dt/NzUwOTc4OTk-/ 7” touchscreen display]50.50
1[http://www.dx.com/p/diy8-x-seven-segment-displays-module-for-arduino-595-driver-250813 8-segment display]3.86
2 [http://www.dx.com/p/arduino-dht11-digital-temperature-humidity-sensor-138531 digital temperature sensor DHT-22]7.50
1 aluminum block 100x100x15 mm14.23
1 [http://www.dx.com/p/tec1-12706-semiconductor-thermoelectric-cooler-peltier-white-157283 Peltier heater 12V 60W]4.49
1power supply32.89
6 [http://www.leds.de/Low-Mid-Power-LEDs/SuperFlux-LEDs/Nichia-Superflux-LED-blau-3lm-100-NSPBR70BSS.html superflux LED 480nm]7.50
16 [http://www.leds.de/Low-Mid-Power-LEDs/SuperFlux-LEDs/Nichia-Superflux-LED-blau-3lm-100-NSPBR70BSS.html LED 450nm]20.00
1cupboard button1.24
-total293.16
Aachen Device Fritzing.png
Wiring of our device
Aachen Device 1.jpg Start building your own WatsOn by assembling the base plate, the sides and the interior wall.
350px] [File:Aachen_Device_.3jpg Fix the Peltier heater on the back of the aluminum block and place it in the hole of the interior wall.

Arrange the 4x4 450nm LEDs and the 2x3 480nm LEDs

Aachen Device 7.jpg Assemble the camera holder with the camera and the corresponding filter slide on the lower part. Over the camera you can place the temperatur sensor for measuring the indoor temperature. Finally put the fan on the back of the camera holder.
Aachen Device 8.jpg Connect the electronic components on the outside and the inside according to the wiring diagramm.
Aachen Device 4.jpg Put together the drawer.
Aachen Device 9.jpg Position the front panel and insert the drawer.
Aachen Device 10.jpg Place the temperatur sensor for the aluminium block tempteratur directly on the block and put the back panel in front of it.
Aachen Device 11.jpg Add the display and apply some stickers to enjoy your custom-made WatsOn.

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