Parts:

Hyaluronic acid production

Number: BBa_K1469001

Description:
Hyaluronan synthase 2 gene of Heterocephalus glaber with codon adaption for expression in Bacillus megaterium. The enzyme catalyzes the linkage of UDP-D-glucuronic acid and UDP-N-acetyl-D-glucosamine to the linear high molecular mass hyaluronic acid chain and is also involved in its secretion.

Sequence:

ATGCACTGTGAACGTTTCTTATGTATTTTACGTATTATTGGTACAACATTATTCGGTGTATCTTTATTATTAGGTATTACAGCTGCTTACATTGTA
GGTTACCAATTCATTCAAACAGATAACTACTACTTCTCTTTCGGTTTATACGGTGCTTTCTTAGCTTCTCACTTAATTATTCAATCTTTATTCGCT
TTCTTAGAACACCGTAAAATGAAAAAATCTTTAGAAACACCAATTAAATTAAACAAAACAGTAGCTTTATGTATTGCTGCTTACCAAGAAGATCCA
GATTACTTACGTAAATGTTTACAATCTGTAAAACGTTTAACATACCCAGGTATTAAAGTAGTAATGGTAATTGATGGTAACTCTGATGATGATTTA
TACATGATGGATATTTTCTCTGAAGTAATGGGTCGTGATAAATCTGCTACATACATTTGGAAAAACAACTTCCACGAAAAAGGTCCAGGTGAAACA
GATGAATCTCACAAAGAATCTTCTCAACACGTAACACAATTAGTATTATCTTCTAAATCTGTATGTATTATGCAAAAATGGGGTGGTAAACGTGAA
GTAATGTACACAGCGTTTCGTGCTCTCGGTCGTTCTGTTGATTACGTACAAGTATGTGATTCTGATACAATGTTAGATCCAGCTTCTTCTGTAGAA
ATGGTAAAAGTATTAGAAGAAGATCCAATGGTAGGTGGTGTAGGTGGTGATGTACAAATTTTAAACAAATACGATTCTTGGATTTCTTTCTTATCT
TCTGTACGTTACTGGATGGCTTTCAACATTGAACGTGCTTGTCAATCTTACTTCGGTTGTGTACAATGTATTTCTGGTCCATTAGGTATGTACCGT
AACTCTTTATTACACGAATTTGTAGAAGATTGGTACTCTCAAGAATTTATGGGTAACCAGTGCAGCTTCGGTGATGATCGTCACTTAACAAACCGT
GTATTATCTTTAGGTTACGCTACAAAATACACAGCTCGTTCTAAATGTTTAACAGAAACACCAATTGAATACTTACGTTGGTTAAACCAACAAACA
CGTTGGTCTAAATCTTACTTCCGTGAATGGTTATACAACGCTATGTGGTTCCACAAACACCACTTATGGATGACATACGAAGCGGTAATAACAGGC
TTCTTCCCATTCTTTTTAATTGCTACAGTAATTCAATTATTCTACCGTGGTAAAATTTGGAACATTTTATTATTCTTATTAACAGTACAATTAGTA
GGTTTAATAAAATCTTCTTTCGCTTCTTGTTTACGTGGTAACATTGTAATGGTATTCATGTCTTTATACTCTGTATTATACATGTCTTCTTTATTA
CCAGCTAAAATGTTCGCTATTGCTACAATTAACAAAGCTGGTTGGGGTACATCTGGTCGTAAAACAATTGTAGTAAACTTCATTGGTTTAATTCCA
GTATCTGTATGGTTCACAATTTTATTAGGTGGTGTAATTTTCACAATTTACAAAGAATCTAAAAAACCATTCTCTGAATCTAAACAAACAGTATTA
ATTGTAGGTACATTATTATACGCTTGTTACTGGGTAATGTTATTAACATTATACGTAGTATTAATAAACAAATGTGGTCGTCGTAAAAAAGGTCAA
CAATACGATATGGTATTAGATGTATAA

Characterisation status:

• Part is user sequenced: no illegal restriction sites, no mutations
• Protein expression successful
• No catalytic activity probably due to misfolding and intracellular accumulation

Pathway engineering for optimsation of Hyaluronic acid production

Number: BBa_K1469002

Description:
UDP-glucose 6-dehydrogenase of Bacillus megaterium. The enzyme catalyzes the NAD dependent oxidation of UDP-glucose to UDP-glucuronic acid, a key precursor molecule for hyaluronic acid production.
Sequence:

Characterisation status:

• Part is user sequenced: no illegal restriction sites, no mutations
• Protein expression successful // LINK TO [SDS PAGE Teil 4]
• characterisation of catalytic activity not possible because no HA production for comparison of HA yields after co expression with hyaluronan synthase 2.

Number: BBa_K1469003

Description: Glucokinase of Bacillus megaterium. The enzyme catalyzes the phosphorylation reaction of glucose to glucose 6-phospahate. Sequence:
//EHER LINK ZU [http://parts.igem.org/Part:BBa_K1469003] ATGGATGACAAATGGTTAGTTGGAGTTGATTTAGGCGGTACAACAATAAAAATGGCCTTTATTAATCATTATGGAGAAATCATTCACAAGTGGGAAATTAATACGGATGTGAGCGAGCAAGGCCGTAAAATTCCAACGGATATTGCAAAAGCAATTGATAAAAAGTTAAACGATCTTGGAGAAGTAAAATCAAGGTTAGTAGGAATTGGCATTGGTGCACCGGGGCCTGTCAACTTTGCAAACGGTTCGATTGAAGTAGCTGTCAATTTAGGTTGGGAAAAATTCCCTATAAAAGATATCTTGGAAGTAGAAACTTCTCTTCCTGTTGTAGTAGACAATGATGCAAACATTGCAGCGATTGGAGAAATGTGGAAGGGTGCTGGAGACGGAGCAAAAGATTTACTTTGCGTTACGCTTGGCACAGGCGTTGGCGGTGGCGTCATTGCAAACGGTGAAATTGTACAAGGCGTAAATGGAGCCGCTGGTGAGATCGGGCACATTACTTCTATTCCTGAAGGCGGGGCACCGTGTAACTGCGGTAAAACCGGCTGTTTAGAAACCATTGCTTCAGCAACTGGAATTGTACGTTTAACAATGGAAGAATTAACGGAAACGGACAAACCAAGTGAGCTTCGCACAGTGTTAGAACAAAATGGACAAGTTACATCTAAAGATGTATTTGATGCAGCTCGTTCAAAAGACGGGTTAGCTATGCATGTTGTAGATAAAGTTGCTTTTCATTTAGGTCTAGCACTAGCAAACTCTGCTAATGCATTAAACCCTGAGAAGATCGTTCTAGGCGGCGGTGTGTCTCGTGCAGGCGAGGTATTACTTGCACCGGTAAGAGATTATTTCAAACGTTTTGCATTTCCTCGCGTAGCGCAAGGTGCTGAACTAGCAATCGCTACTTTAGGAAACGATGCGGGAATTATTGGAGGAGCTTGGTTAGTTAAATCTTATTTTGAATAA

Characterisation status:

• Part is user sequenced: no illegal restriction sites, no mutations
• Part is not RFC10 conform.
• As indicated on the vector map: GCGGCCGCTTCTAG of prefix and TACTAGTAGCGGCCG of suffix are deleted
• Use only after amplification with appropriate primers

Number: BBa_K1469004

Description: Beta-phosphoglucomutase of Bacillus megaterium. The enzyme catalyzes the conversion of glucose 6-phospahate to glucose 1-phosphate. Sequence: //EHER LINK ZU [http://parts.igem.org/Part:BBa_K1469004] ATGAAAAAAGAAGAGCTACAAGCGATTATTTTTGATTTAGACGGTGTTATTGCAGATACGGTACATTTATATTATAAAGCAAATAAAAAAGTAGCAGATCAACTTAACGTTTCATTTTCTGAAGAACTCAATCAGCAGCTGCAGGGGATCAGCCGAGTGAAAACTGTAGAGCTCATTGCTGCCCAAGGCAATGTACATCTGACAATGGAAAAAAAAGAACAGCTTGCTGATGAAAAGAATAAGCATTATCAAGCATTAATAAAGGAAATGACAAAGGATCATCTACTTCCAGGAATACAATTGTTGTTAGAGGATTGTAAAAAACAAGGTATTAAAATGGCGATTGCTTCTTCTAGTTCAAATGCAAATACTGTAGTGGATGCGTTAGGAATACGTCCTTTTTTTGATTGCATTGTGGATGTGAGAAAAATAAAGAAGGGAAAACCAGACCCAGAAATATTTTTGACAGCTGCCCATCAATTAAAAGTAACACCCTATGCATGTGTGGCTATAGAAGATGGAGAGGCGGGTGTAAAAGCTATTAATCAAACAGATATGGTTTCTATCGGCGTTGGTAAGCATCTGAAGGAAGAGGATATGAATTTTCACGTATATTCAACAGCTGATTTGCATTTAAAGACGATCAAAGAGGTTTTCGAAAAAACAAGAAAAGGAACAGATTATAACAGCGTGTATACAAAAAATTAA

Characterisation status:

• Part is user sequenced: no illegal restriction sites, no mutations
• Part is not RFC10 conform
• As indicated on the vector map: GCGGCCGCTTCTAG of prefix is deleted
• Use only after amplification with appropriate primers

Number: BBa_K1469005

Description: UTP--glucose-1-phosphate uridylyltransferase of Bacillus megaterium. The enzyme catalyzes the conversion of glucose 1-phospahate to UDP-glucose 1-phosphate. Sequence: // EHER LINK ZU [http://parts.igem.org/Part:BBa_K1469005] ATGACGATAAAAAAGGCAGTTATTCCAGTAGGCGGTTTAGGAACTCGATTTTTGCCAGCGACAAAAGCGCAGCCCAAAGAAATGCTGCCAATTGTCGATAAGCCTGCTGTACAATATATTGTAGAAGAAGCTGTGAAATCAGGTATTGAAAGTATTATTTTTATTACAGGACGAAACAAAAAATCAATTGAAGACCATTTTGATAAATCAACTGAGTTAGAGCAAATGCTTGAAGAAAAACACAAGTTCCAAATGCTAAAAGAAGTACAAACGATCTCATCAATGGCCAGCATTCACTATATTCGTCAAAAAGAGCCGTTAGGACTTGGTCATGCTATTCTTTGTGCCGAACAGTTTATTGGAGATGAGCCGTTTGCCGTTCTTCTTGGAGACGATATTATGACTTCTGAAGAACCTGCGCTCAAACAGATAATCCAAGCGTATGAAACGACTAATCAATCAGTTATCGGAGTTCAAAAAGTGGATGTTGAAGAGGTATCAAAATACGGAATCATTCAGCCGAAAAATAAATCTGGCAGCCTTCATGAAGTGAATGACTTGATTGAAAAACCATCGATAGAACAAGCTCCGTCAACTATTGCCGTTATGGGCCGCTATATTTTAAATCCTTCTATTTTTTCTTATCTAAAAACGATTGAAAGAGGGGTAGGAAATGAATTACAATTAACAGATGCACTGGGTGTGGTATGTGAAAAAGAGCGATTGTTTGCTCTTGAACTGGAAGGACAGCGCTTTGATATTGGAGATAAGGTAGGCTATATGAAAGCGATGGTAGAAGTTGCGTTAAAGCGCAGAGATTTACGTCAAACGTTTTTATCGTATTTGGAGGGAATTGTAGAAAAAGAACGAGCAAACAACTTCAGCTAA Characterisation status:

• Part is user sequenced: no illegal restriction sites, no mutations
• Part is not RFC10 conform
• As indicated on the vector map: GCGGCCGCTTCTAG of prefix and TACTAGTAGCGGCCG of suffix are deleted
• Use only after amplification with appropriate primers

Number: BBa_K1469006

Description: Glucose-6-phosphate isomerase of Bacillus megaterium. The enzyme catalyzes the conversion of glucose 6-phospahate to fructose 6-phosphate.

Sequence: // EHER LINK ZU [http://parts.igem.org/Part:BBa_K1469006] ATGACACATGTTCGTTTTGATTATTCAAACGCGTTATCATTTTTTGGTGAACATGAACTTACATACTTACGTGATGCCGTGAAAGTAGCTCACCATTCAATTCATGAGAAAACAGGTGCTGGAAACGACTTTTTAGGATGGGTCGACCTTCCTACGGAGTATGACAAAGAAGAATTTGCACGCATTCAAAAAAGTGCAGAAAAAATTAAATCTGATTCAGATGTATTAATTGTTATCGGTATTGGTGGATCTTACTTAGGTGCTCGTGCTGCTATTGAAATGCTTCAGCATTCATTCTATAACACATTATCAAAAGAACAGCGTAAAACTCCGCAAGTTATTTTTGCTGGTAACAACATCAGCTCAACATACATGAAAGACTTAATGGATGTTTTACAAGATAAAGATTTCTCTATTAATGTTATTTCAAAATCTGGTACAACAACAGAACCGGCGATTGCTTTCCGTATCTTCCGTAAATTATTAGAAGAGAAATACGGCAAAGAAGAAGCACGCAAGCGTATTTATGCAACAACAGATAAAGCGCGCGGTGCATTAAAAACATTAGCGGACGAAGAAGGCTATGAAACATTTGTTATTCCAGATGATGTAGGCGGCCGTTATTCAGTATTAACAGCGGTTGGCTTATTACCAATTGCCGCTGCGGGTCTTGATATCGAACAAATGATGAAAGGGGCAGCTGATGCTCAAGAAGCATTCTCATCATCAGAGCTAGAAGAGAACCCAGCTTATCAATATGCTGTAGCTCGTAATGTGCTTTATAATAAAGGAAAAACAATTGAAATGCTGATTAACTATGAGCCAGCTCTTCAATACTTTGCTGAGTGGTGGAAACAGTTATTTGGCGAAAGTGAAGGAAAAGACCAAAAAGGTATTTACCCTTCATCTGCTAACTTCTCAACGGATCTTCATTCTTTAGGTCAATATGTACAAGAAGGTCGCCGTGATATTTTTGAAACAATTGTACAAGTTCAAGATTCTCGTCATGAGCTAACAATTGAAGAAGATGCAGCGGACTTAGATGGATTAAATTACTTAGCTGGTGAAACAGTTGACTTTGTAAATAAAAAAGCGTTCCAAGGTACAATGCTTGCTCACACAGATGGAGGCGTTCCAAACTTAGTTGTGAACATTCCTGCGCTAGATGAGTATACGTTCGGATATGTTGTATATTTCTTCGAAAAAGCATGTGCAATCAGCGGCTATTTATTAGGCGTAAACCCATTCGATCAGCCGGGAGTAGAAGCATACAAAGTAAATATGTTTGCTCTTCTTGGAAAACCTGGTTTTGAAGAGAAAAAAGCAGAATTAGAAAAGCGTTTGAAATAA

Characterisation status:

• Part is user sequenced: no illegal restriction sites, no mutations
• Part is not RFC10 conform
• As indicated on the vector map: TACTAGTAGCGGCCG of suffix is deleted
• Use only after amplification with appropriate primers

Number: BBa_K1469007

Description: Glucosamine--fructose-6-phosphate aminotransferase (isomerising) of Bacillus megaterium. The enzyme catalyzes the conversion of fructose 6-phosphate to glucosamine 6-phosphate. Sequence: // EHER LINK ZU [http://parts.igem.org/Part:BBa_K1469007] ATGTGCGGAATTGTAGGATATATTGGAACAGAAGATTCGAAAGAAATTTTATTACGTGGTCTTGAAAAGTTAGAATATCGTGGCTATGACTCAGCGGGTATTGCAGTTGTTAACAATGAAGGCGTACACGTATTCAAAGAAAAAGGCCGTATTGCAGAATTGCGTCAGGCAGTAGATAACAGTGTTGTAGCACCAGCAGGTATCGGTCATACGCGTTGGGCAACTCATGGTGTACCAAGCCAAGAAAATGCTCACCCGCATCAAAGTACATCTGGACGCTTCACACTTGTGCATAACGGTGTAATTGAAAACTATGCTATTTTGAAACGTGAATATTTACAAGATGTAACGTTCAAAAGCGAAACGGATACAGAAGTAATCGTTCAGTTAATTCAAAAAATCGCAGCAGAAGGCTTAGATGTAGAAGAAGCGTTCCGTAAGACAGTAAGCTTGCTTCATGGTTCTTATGCACTAGCTCTTGTTGATAACAAAGATAAAAATACAATCTACGTAGCTAAAAACAAAAGCCCGCTTCTTGTAGGTGTTGGTGAAGGATTTAACGTTGTAGCGAGTGATGCTATGGCAATGCTTCAAGTAACAGATCAGTACGTAGAATTAATGGATAAAGAAACAGTAATTGTTACAAAAGAAGGCGTAACAATTAAAACGCTTGATGGTGAAGTGGTAGAAAGAGCACCTTATACAGCAGAGCTTGATGCAAGTGATATTGAAAAAGGTACGTACCCTCACTATATGTTAAAAGAAATTGACGAGCAGCCTTTAGTTGTTCGTAAAATCATTCAAGAGTATCAAAATGAGAACAATGAACTGCACATTGATGCAGACATTTTAGGAGCAATGGATGAAGCAGACCGCATCTATATCGTAGCATGTGGAACAAGCTATCATGCAGGCCTAGTAGGTAAACAATTCATTGAAACGTGGGCGAAGGTTCCAGTAGAAGTGCACGTAGCAAGTGAATTTTCTTATAACATGCCGCTTTTATCTGAAAAGCCATTGTTTATCTTTATTTCTCAAAGTGGAGAAACAGCTGACAGCCGTGCAGTATTAGTTCAAATTAAAGAACTAGGTTACAAAGCGTTAACAATCACAAACGTTCCGGGATCAACATTATCTCGTGAATCTGATTATACATTATTACTTCATGCAGGTCCGGAAATTGCTGTAGCATCAACAAAAGCTTATACAGCTCAATTAGCAGTGCTATCAATTCTAGCGGCTGTAACAGCAAAAACACGCGGCATTGAATTAGAATTTGATTTAGTTCAAGAGTTAGCAATCGTAGCAAACACAATGGAAGTACTTTGCGACGACAAAGAAGAAATGGAAAGCATTGCTCTTCAATTCTTAGCAACAACTCGCAACGCATTCTTTATTGGACGTTCTGTCGATTATTATGTAGGTTTAGAAGGTGCGTTAAAACTAAAAGAAATCTCTTACATCCAAGCAGAAGGATTTGCTGGAGGAGAGCTTAAGCACGGAACAATCGCTTTAATTGAAAACAATACGCCAATTATTGCATTAGCAACACAAGAGCATGTAAACTTAAGCATCCGTGGTAACGTAAAAGAAGTAGTAGCACGTGGAGCTAATCCTTGTATCATTTCAATGAAAGGATTAGAAACAGAAGAAGATCGCTACGTGATTCCGAAAGTTCACGAAACACTTTCACCACTAGCAGCGGTTATTCCTTTACAATTAATCTCTTACTACGCTGCATTGCACCGCGATTGCGATGTAGATAAACCACGTAACTTAGCTAAATCAGTTACTGTAGAA TAA Characterisation status:

• Part is user sequenced: no illegal restriction sites, no mutations
• Part is not RFC10 conform
• As indicated on the vector map: TACTAGTAGCGGCCG of suffix is deleted
• Use only after amplification with appropriate primers

Number: BBa_K1469008

Description: Phosphoglucosamine mutase of Bacillus megaterium. The enzyme catalyzes the conversion of glucosamine 6-phosphate to glucosamine 1-phosphate. Sequence: // EHER LINK ZU [http://parts.igem.org/Part:BBa_K1469008] ATGGGTAAGTATTTTGGAACAGACGGAGTACGAGGAGTTGCCAATAGTGAATTAACTCCTGAGCTTGCTTTTAAAATTGGACGCTTAGGTGGTTACGTTCTAACAAAAGATTCAGAGCGACCAAAAGTATTAATCGGACGCGATACACGCGTTTCTGGTCATATGTTAGAAGGAGCGCTTGTTGCAGGATTGCTTTCAATTGGAGCAGAAGTTATGCGACTAGGTGTTATTTCAACTCCAGGGGTAGCGTATTTAACAAAAGCACTTGGCGCACAAGCAGGAGTCATGATTTCTGCTTCGCATAACCCAGTTCAAGATAACGGAATTAAATTCTTTGGTCCAGATGGATTTAAACTTTCAGATGCCCAAGAGAATGAAATTGAAGCGTTAATGGATAGTGACACAGATCAATTACCAAGACCTGTTGGGGGAGACTTAGGTCAGGTAAATGACTATTTCGAAGGTGGACAAAAATATCTTCAATACTTAAAACAAACGGTTGATGAAGACTTCTCAGGTATTCATGTGGCATTAGACTGTGCGCACGGGGCAACGTCATCGTTAGCAGCACATTTATTTGCAGATCTCGATGCAGATATCTCTACAATGGGGGCATCTCCAAACGGATTGAACATCAATGACGGTGTAGGATCTACACATCCGGAAGCATTAAGTGCCTTCTTAAAAGAAAAAGGAGCGGATGTTGGATTAGCATTTGATGGAGACGGAGATCGTTTAATCGCAATCGACGAAAAAGGCGAGATTGTAGACGGTGATCAAATTATGTTCATTTGTGCAAAATACTTATATGCACAAGGTCGTTTAAAGAAAAACACGCTTGTTTCTACCGTTATGAGTAACTTAGGCTTCTATAAAGCACTAGAAGCTAGCGGGATTGAAAGTGCACAAACTGGTGTAGGAGACCGTTATGTAGTAGAAGAAATGAAAGCTAATCAATATAACCTAGGTGGAGAACAGTCAGGTCATATTATTTTCTTAGATTACAACACAACAGGTGACGGTATGCTTTCAGCTATTCAGCTTGTAAACATTATGCAAGCAACGAAAAAGCCTTTATCAGAATTAGCAGCTGAAATGAAGAAATATCCTCAGCTTCTTGTAAATGTAAAAGTAACAGATAAGCATCATGTTACAGATAACGAGAAGGTAAAAGTGGTTATTGAAGAAGTGGAAAAAGAAATGAACGGTAATGGCCGAGTACTTGTACGCCCTTCAGGAACAGAGCCGTTAGTACGTGTGATGGTTGAAGCACAAACACAAGAAGAATGTGAAACTTATGTAACGCGTATCGTAGAAGTAGTAAAAGAAGAAATGGGCTTAGAGTAA

Characterisation status:

• Part is user sequenced: no illegal restriction sites, no mutations
• Part is not RFC10 conform
• As indicated on the vector map: GCGGCCGCTTCTAG of prefix and TACTAGTAGCGGCCG of suffix are deleted
• Use only after amplification with appropriate primers

Number: BBa_K1469009

Description: UDP-N-acetylglucosamine pyrophosphorylase of Bacillus megaterium. The enzyme is bifunctional and catalyzes the conversion of glucosamine 1-phosphate to N-acetyl glucosamine 1-phosphate as well as the conversion of N-acetyl glucosamine 1-phosphate to UDP-N-acetyl glucosamine, a key precursor molecule for hyaluronic acid production. Sequence: // EHER LINK ZU [http://parts.igem.org/Part:BBa_K1469009] ATGTCAAAAAGATATGCAGTCATATTGGCAGCTGGTCAGGGTACACGTATGAAGTCATCTTTATATAAAGTGTTACACCCTGTGTGTGGCAAACCGATGGTGCAACACGTAATTGATCAGTTATCACGTTTGGATTTAACAAGTTTAATTACAGTTGTAGGTCACGGTGCCGAAAAAGTGAAATCACATGTTGGAGATAAAAGCTTATTTGCTTTACAAGAAGAGCAGTTAGGAACAGCTCATGCAGTAATGCAAGCAGAATCTTTACTTGCACATGAAAAAGGTACGACAATTGTTGTATGTGGAGATACTCCGCTTATTACAGCTGAAACAATTGAATCTTTATTAAAGCATCATGAAGCAACAAATGCAATGGCAACTATTTTAACAGCTCATGCAGAAGATCCAACAGGATATGGACGCATCATTCGAAATGAACAAGGGTCTGTTGAAAAGATTGTGGAGCATAAAGATGCCACAGAACAAGAGCGTCTTGTACAGGAAATTAATACTGGTACGTATTGTTTTGATAATGAGAAATTATTTGAGACACTTTCAAAAGTTTCAAATGATAATGTACAGGGAGAGTATTATTTACCTGATGTTATCGAAATTCTAAAAAATGAAGGTCAAATTATTTCTGCATATCAAACATCCGATTTTGCTGAAACATTGGGCGTTAATGATCGATTTGCTCTGTCTCAAGCAGAAGAAACAATGAAAAAGCGCATCAACAAGCAGCATATGTTAAATGGAGTGACGATTGTGGATCCTTCTAACACGTACATTTCAGCAGATGCTGTCATTGGCAGAGATACGGTTATTTATCCTGGTACGGTTATTCAAGGAACAGTTGTAATTGGAGAGAATTGTGAAGTAGGACCTAACAGTGAAATTAAAGACTGCAAAATAGGTAATAATACTTCTATTCGCCATTCTGTTGCTCACGATAGTGAAATTGGTCACGAAGTCACAATTGGCCCATTTGCACATATTCGTCCTCAATCATTAATTGGAGATGAAGTGCGCGTAGGAAACTTTGTAGAAATTAAAAAAGCATCTTTTGGCAAAGGTAGCAAAGCTTCTCATTTAAGTTATATTGGAGACGCAGAGGTCGGAAAAGGTGTAAACTTAGGGTGCGGATCTATTACAGTTAACTACGATGGGAAAAATAAATTTTTAACAAAGATTGAAGATGGGGCATTCGTAGGATGTAACTCAAACTTAATTGCACCTGTAACAATTGGTGAAGGCGCCTATGTTGCAGCTGGTTCAACCGTAACGGATGATGTGCCAGGAAAAGCATTATCAATTGCTCGTGCGAAACAAGTAAATAAAGAAAACTATGCTGAAAAGCTTGATATTAATAAAAAATCCTAA Characterisation status:

• Part is user sequenced: no illegal restriction sites, no mutations
• Part is not RFC10 conform
• As indicated on the vector map: GCGGCCGCTTCTAG of prefix and TACTAGTAGCGGCCG of suffix are deleted
• Use only after amplification with appropriate primers

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