Team:Aachen/Collaborations/Heidelberg

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The iGEM team Heidelberg constructed a set of expression vectors that contain a T7 promoter prior to the BioBrick prefix. This way RBS-CDS parts can be cloned into the vectors by standard BioBrick assembly and then expressed in BL21(DE3) by induction with IPTG.
The iGEM team Heidelberg constructed a set of expression vectors that contain a T7 promoter prior to the BioBrick prefix. This way RBS-CDS parts can be cloned into the vectors by standard BioBrick assembly and then expressed in BL21(DE3) by induction with IPTG.
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{{Team:Aachen/Figure|Aachen_Heidelberg_vector_Design.png|title=Heidelberg X-press vector design|subtitle=The iGEM Team Heidelberg created new expression vectors with an integrated T7 Promoter|width=900px}}
{{Team:Aachen/Figure|Aachen_Heidelberg_vector_Design.png|title=Heidelberg X-press vector design|subtitle=The iGEM Team Heidelberg created new expression vectors with an integrated T7 Promoter|width=900px}}
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In collaboration with the iGEM Team Heidelberg the iGEM Team Aachen tested 8 of these vectors for their expression capabilities.
In collaboration with the iGEM Team Heidelberg the iGEM Team Aachen tested 8 of these vectors for their expression capabilities.

Revision as of 08:25, 13 October 2014

X-press: Characterization of Team Heidelbergs pSBX-vectors

The iGEM team Heidelberg constructed a set of expression vectors that contain a T7 promoter prior to the BioBrick prefix. This way RBS-CDS parts can be cloned into the vectors by standard BioBrick assembly and then expressed in BL21(DE3) by induction with IPTG.

Aachen Heidelberg vector Design.png
Heidelberg X-press vector design
The iGEM Team Heidelberg created new expression vectors with an integrated T7 Promoter

In collaboration with the iGEM Team Heidelberg the iGEM Team Aachen tested 8 of these vectors for their expression capabilities.

The Team Heidelberg expression vectors

These are the tested constructs:

Plasmid Backbone Insert Length vector [bp] Length Insert [bp] Resistance
pSBX1A3-BBa_13507 pSBX1A3 (high copy) BBa_13507 3067 1226 50 mg/ml Ampicilin
pSBX1C3-BBa_13507 pSBX1C3 (high copy) BBa_13507 2982 1226 35 mg/ml Chloramphenicol
pSBX1K3-BBa_13507 pSBX1K3 (high copy) BBa_13507 3116 1226 50 mg/ml Kanamycin
pSBX1T3-BBa_13507 pSBX1T3 (high copy) BBa_13507 3322 1175 10 mg/ml Tetracyclin
pSBX4A5-BBa_13507 pSBX4A5 (low copy) BBa_13507 4307 1192 50 mg/ml Ampicilin
pSBX4C5-BBa_13507 pSBX4C5 (low copy) BBa_13507 4127 1186 15 mg/ml Chloramphenicol
pSBX4K5-BBa_13507 pSBX4K5 (low copy) BBa_13507 4331 1192 50 mg/ml Kanamycin

We also used an mRFP selection marker with a constant expression of mRFP as a positive control.


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