Team:Bielefeld-CeBiTec/Notebook/StrainsAndConstructs

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<td width="30%" id=""> <a href=http://www.promega.de/resources/protocols/technical-bulletins/101/single-step-krx-competent-cells-protocol> <i>Escherichia coli</i> KRX </a></td>
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<td width="40%">[F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase</td>
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<td width="30%">Promega</td>
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Revision as of 15:03, 11 October 2014


Strains and Constructs


Name Properties BioBrick
General
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Module 1
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Module 2
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Module 3
JW1228-1 ΔadhE748::kan Baba et al., 2006
Biosafety
Escherichia coli KRX [F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase Promega
References
  • Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11