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Team:Aix-Marseille/Attributions
From 2014.igem.org
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<span class="project-tag" id="labwork"></span> | <span class="project-tag" id="labwork"></span> | ||
| - | < | + | <h2>Lab work</h2> |
<ul> | <ul> | ||
<li> | <li> | ||
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<ul> | <ul> | ||
<li><u>Serine production:</u> Aimeric, Alexia, Clara</li> | <li><u>Serine production:</u> Aimeric, Alexia, Clara</li> | ||
| - | <li><u>ppGpp biosynthesis and degradation (RelA, | + | <li><u>ppGpp biosynthesis and degradation (RelA, Mesh2):</u> Alexia</li> |
<li><u>CheA-CusR hybrid two component system:</u> Alexia, Clara, Lambert</li> | <li><u>CheA-CusR hybrid two component system:</u> Alexia, Clara, Lambert</li> | ||
<li><u>GFP/RFP reporter system:</u> Clara, Lambert, Alexia</li> | <li><u>GFP/RFP reporter system:</u> Clara, Lambert, Alexia</li> | ||
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<div class="project-section"> | <div class="project-section"> | ||
<span class="project-tag" id="modeling"></span> | <span class="project-tag" id="modeling"></span> | ||
| - | < | + | <h2>Project Modeling</h2> |
<p>Vincent</p> | <p>Vincent</p> | ||
</div> | </div> | ||
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<div class="project-section"> | <div class="project-section"> | ||
<span class="project-tag" id="wiki"></span> | <span class="project-tag" id="wiki"></span> | ||
| - | < | + | <h2>Wiki design, coding and filling</h2> |
<p>Romain</p> | <p>Romain</p> | ||
</div> | </div> | ||
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<div class="project-section"> | <div class="project-section"> | ||
<span class="project-tag" id="safety"></span> | <span class="project-tag" id="safety"></span> | ||
| - | < | + | <h2>Safety</h2> |
<p>Clara, Lambert, Aimeric, Vincent, Romain, Alexia</p> | <p>Clara, Lambert, Aimeric, Vincent, Romain, Alexia</p> | ||
</div> | </div> | ||
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<div class="project-section"> | <div class="project-section"> | ||
<span class="project-tag" id="comm_fund"></span> | <span class="project-tag" id="comm_fund"></span> | ||
| - | < | + | <h2>Communication and funding prospection</h2> |
<p>Clara, Lambert, Aimeric, Vincent, Romain, Alexia</p> | <p>Clara, Lambert, Aimeric, Vincent, Romain, Alexia</p> | ||
</div> | </div> | ||
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<div class="project-section"> | <div class="project-section"> | ||
<span class="project-tag" id="social_comm"></span> | <span class="project-tag" id="social_comm"></span> | ||
| - | < | + | <h2>Social network communication</h2> |
<p>Romain</p> | <p>Romain</p> | ||
</div> | </div> | ||
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<div class="project-section"> | <div class="project-section"> | ||
<span class="project-tag" id="jamboree"></span> | <span class="project-tag" id="jamboree"></span> | ||
| - | < | + | <h2>Poster and oral communication at the iGEM Jamboree</h2> |
<p>Alexia, Vincent, Aimeric</p> | <p>Alexia, Vincent, Aimeric</p> | ||
</div> | </div> | ||
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<div class="project-section"> | <div class="project-section"> | ||
<span class="project-tag" id="AMU_team"></span> | <span class="project-tag" id="AMU_team"></span> | ||
| - | < | + | <h2>Creation of the iGEM-AMU association and Team member recruitment</h2> |
<p>Clara, Lambert, Aimeric, Romain</p> | <p>Clara, Lambert, Aimeric, Romain</p> | ||
</div> | </div> | ||
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<div class="project-section"> | <div class="project-section"> | ||
<span class="project-tag" id="advisors"></span> | <span class="project-tag" id="advisors"></span> | ||
| - | < | + | <h2>Advisors</h2> |
<p>We want to thank our advisors who gave us time and support during the different steps of the project, from Team construction to final Jamboree preparation.<p> | <p>We want to thank our advisors who gave us time and support during the different steps of the project, from Team construction to final Jamboree preparation.<p> | ||
<p>James Sturgis participated in the initial project brainstorming, advised on parts design, experimental procedures, interpretation and presentation of results.</p> | <p>James Sturgis participated in the initial project brainstorming, advised on parts design, experimental procedures, interpretation and presentation of results.</p> | ||
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<div class="project-section"> | <div class="project-section"> | ||
<span class="project-tag" id="collaborators"></span> | <span class="project-tag" id="collaborators"></span> | ||
| - | < | + | <h2>Collaborators</h2> |
<p>We are very grateful to Dr. Bouveret who kindly shared her expertise in ppGpp metabolism, established the experimental design and did the validation of the BBa_K1349001 part.</p> | <p>We are very grateful to Dr. Bouveret who kindly shared her expertise in ppGpp metabolism, established the experimental design and did the validation of the BBa_K1349001 part.</p> | ||
</div> | </div> | ||
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<div class="project-section"> | <div class="project-section"> | ||
<span class="project-tag" id="ack"></span> | <span class="project-tag" id="ack"></span> | ||
| - | < | + | <h2>Acknowledgments</h2> |
<ul> | <ul> | ||
<li> | <li> | ||
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<li><p>Plasmids pWW2181, pWW2179 and pWW2021 used as matrix to construct the CheA-CusR hybrid two component system (parts BBa_K1349005, BBa_K1349007, BBa_K1349008) are a generous gift from Dr. J. Dueber (Berkeley University, USA). Related publication: Whitaker et al. PNAS 2012.</p></li> | <li><p>Plasmids pWW2181, pWW2179 and pWW2021 used as matrix to construct the CheA-CusR hybrid two component system (parts BBa_K1349005, BBa_K1349007, BBa_K1349008) are a generous gift from Dr. J. Dueber (Berkeley University, USA). Related publication: Whitaker et al. PNAS 2012.</p></li> | ||
<li><p>Plasmid template for RelA part construction (BBa_K1349001) is the work of Dr. E. Bouveret (LISM, Marseille).</p></li> | <li><p>Plasmid template for RelA part construction (BBa_K1349001) is the work of Dr. E. Bouveret (LISM, Marseille).</p></li> | ||
| - | <li><p>The original plasmid carrying | + | <li><p>The original plasmid carrying mesh2 and used as a template to build our mesh2 part (BBa_K1349004) was kindly provided by Dr. B. Field (Laboratoire de Génétique et Biophysique des Plantes, Marseille).</p></li> |
</ul> | </ul> | ||
</li> | </li> | ||
Latest revision as of 13:11, 11 October 2014
Attributions
Lab work
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Part design
Clara, Lambert, Aimeric, Vincent, Alexia
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Chassis Construction
Aimeric
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Sub-projects
- Serine production: Aimeric, Alexia, Clara
- ppGpp biosynthesis and degradation (RelA, Mesh2): Alexia
- CheA-CusR hybrid two component system: Alexia, Clara, Lambert
- GFP/RFP reporter system: Clara, Lambert, Alexia
Project Modeling
Vincent
Wiki design, coding and filling
Romain
Safety
Clara, Lambert, Aimeric, Vincent, Romain, Alexia
Communication and funding prospection
Clara, Lambert, Aimeric, Vincent, Romain, Alexia
Social network communication
Romain
Poster and oral communication at the iGEM Jamboree
Alexia, Vincent, Aimeric
Creation of the iGEM-AMU association and Team member recruitment
Clara, Lambert, Aimeric, Romain
Advisors
We want to thank our advisors who gave us time and support during the different steps of the project, from Team construction to final Jamboree preparation.
James Sturgis participated in the initial project brainstorming, advised on parts design, experimental procedures, interpretation and presentation of results.
Gaël Chambonnier, Sandra Michel and Laetitia Houot trained students for bench work, advised on experimental design, provided technical support and troubleshooting on experiments, advised on interpretation and presentation of results.
Collaborators
We are very grateful to Dr. Bouveret who kindly shared her expertise in ppGpp metabolism, established the experimental design and did the validation of the BBa_K1349001 part.
Acknowledgments
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Host Laboratory and equipments
To develop our project, we were hosted at the Laboratoire d'Ingénierie des Systèmes Macromoléculaires (LISM, UMR7255) CNRS-Aix-Marseille Université in Marseille.
The LISM provided us our own lab space, office space, computers and gave us unlimited access to centrifuges, PCR machines, freezers, incubators, glassware, magic box etc..
We want to thank Olivier, Isabelle and Annick at the LISM for technical assistance in buffers and growth medium preparation, waste management and glassware washing/sterilization.
The Department of Biology in Aix-Marseille University lent all us the regular bench equipment for molecular biology such as small centrifuges, PCR machine, pipetmans, electrophoresis apparatus, etc.
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Ressources
Plasmids pWW2181, pWW2179 and pWW2021 used as matrix to construct the CheA-CusR hybrid two component system (parts BBa_K1349005, BBa_K1349007, BBa_K1349008) are a generous gift from Dr. J. Dueber (Berkeley University, USA). Related publication: Whitaker et al. PNAS 2012.
Plasmid template for RelA part construction (BBa_K1349001) is the work of Dr. E. Bouveret (LISM, Marseille).
The original plasmid carrying mesh2 and used as a template to build our mesh2 part (BBa_K1349004) was kindly provided by Dr. B. Field (Laboratoire de Génétique et Biophysique des Plantes, Marseille).
