"
Team:Hong Kong HKUST/wetlab/notebook
From 2014.igem.org
(Difference between revisions)
| Line 132: | Line 132: | ||
Wet lab<br> | Wet lab<br> | ||
· Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600<br> | · Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600<br> | ||
| - | · | + | · <br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 139: | Line 139: | ||
· Found reference promoter J23101 in BBa_I20260 from part registry<br> | · Found reference promoter J23101 in BBa_I20260 from part registry<br> | ||
· Looked for Pcombox gene sequence from the genomic DNA<br> | · Looked for Pcombox gene sequence from the genomic DNA<br> | ||
| - | · | + | · <br> |
· <br> | · <br> | ||
<br><b>June 11</b> <br> | <br><b>June 11</b> <br> | ||
| Line 145: | Line 145: | ||
· Looked for Pcombox gene sequence from the genomic DNA<br> | · Looked for Pcombox gene sequence from the genomic DNA<br> | ||
· Studied the Characteristic, function and mechnism of how promoters work<br> | · Studied the Characteristic, function and mechnism of how promoters work<br> | ||
| - | · | + | · <br> |
· <br> | · <br> | ||
<br><b>June 12</b> <br> | <br><b>June 12</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
· Looked for Pcombox gene sequence from the genomic DNA<br> | · Looked for Pcombox gene sequence from the genomic DNA<br> | ||
| - | · | + | · <br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 156: | Line 156: | ||
Wet lab<br> | Wet lab<br> | ||
· Looked for Pcombox gene sequence from the genomic DNA<br> | · Looked for Pcombox gene sequence from the genomic DNA<br> | ||
| - | · | + | · <br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 169: | Line 169: | ||
· Blast the gDNA of NCTC7465 strain with various strain of S. pneumoniae to look for Pcombox gene sequence<br> | · Blast the gDNA of NCTC7465 strain with various strain of S. pneumoniae to look for Pcombox gene sequence<br> | ||
· Found a possible Pcombox<br> | · Found a possible Pcombox<br> | ||
| - | · | + | · <br> |
| - | + | ||
· <br> | · <br> | ||
<br><b>June 17</b> <br> | <br><b>June 17</b> <br> | ||
| Line 177: | Line 176: | ||
· Found a possible Pcombox<br> | · Found a possible Pcombox<br> | ||
· Searched for other possible Pcombox sequences<br> | · Searched for other possible Pcombox sequences<br> | ||
| - | |||
| - | |||
· <br> | · <br> | ||
<br><b>June 18</b> <br> | <br><b>June 18</b> <br> | ||
| Line 184: | Line 181: | ||
· Designed primers for the Pcombox sequences<br> | · Designed primers for the Pcombox sequences<br> | ||
· Received a Pcombox sequence from Wellcome Trust Sanger Institute<br> | · Received a Pcombox sequence from Wellcome Trust Sanger Institute<br> | ||
| - | · | + | · <br> |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
<br><b>June 19</b> <br> | <br><b>June 19</b> <br> | ||
· Digestion of pSB1C3-BBa_E0240 at EcoRI and SpeI<br> | · Digestion of pSB1C3-BBa_E0240 at EcoRI and SpeI<br> | ||
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | + | ||
| - | + | ||
| - | + | ||
<br><b>June 20</b> <br> | <br><b>June 20</b> <br> | ||
· Amended primers of Pcombox sequences<br> | · Amended primers of Pcombox sequences<br> | ||
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | · | + | · <br> |
| Line 215: | Line 204: | ||
· Primer design for PCR of comX-myc and comW-FLAG from genomic DNA of S. Pneumoniae NCTC 7465 strain<br> | · Primer design for PCR of comX-myc and comW-FLAG from genomic DNA of S. Pneumoniae NCTC 7465 strain<br> | ||
· Designed plasmid construct for Pcombox<br> | · Designed plasmid construct for Pcombox<br> | ||
| - | · | + | · <br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 226: | Line 215: | ||
· Designed forward and reverse oligos of 67bp Pcombox<br> | · Designed forward and reverse oligos of 67bp Pcombox<br> | ||
· Transformation of pSB1C3-BBa_I13504<br> | · Transformation of pSB1C3-BBa_I13504<br> | ||
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | · | + | · <br> |
· <br> | · <br> | ||
<br><b>June 25</b> <br> | <br><b>June 25</b> <br> | ||
| Line 235: | Line 224: | ||
· Inoculation of BBa_K880005<br> | · Inoculation of BBa_K880005<br> | ||
· Inoculation of pSB1C3-BBa_E0240<br> | · Inoculation of pSB1C3-BBa_E0240<br> | ||
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | · | + | · <br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 246: | Line 235: | ||
· Digestion of BBa_K880005 plasmid extraction product with SpeI and PstI, gel extraction and gel purification<br> | · Digestion of BBa_K880005 plasmid extraction product with SpeI and PstI, gel extraction and gel purification<br> | ||
· Digestion of pSB1C3-BBa_E0240 at XbaI and PstI<br> | · Digestion of pSB1C3-BBa_E0240 at XbaI and PstI<br> | ||
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
<br><b>June 27</b> <br> | <br><b>June 27</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
| Line 259: | Line 242: | ||
· Digestion of pSB3K3-BBa_J04450 at XbaI and PstI<br> | · Digestion of pSB3K3-BBa_J04450 at XbaI and PstI<br> | ||
· Gel purification of pSB1C3-BBa_E0240<br> | · Gel purification of pSB1C3-BBa_E0240<br> | ||
| - | · | + | · <br> |
| Line 325: | Line 308: | ||
· Gel purification of digested BBa_E0240 and BBa_J04450<br> | · Gel purification of digested BBa_E0240 and BBa_J04450<br> | ||
· Inoculation of pSB3K3-BBa_J04450<br> | · Inoculation of pSB3K3-BBa_J04450<br> | ||
| - | · | + | · <br> |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 338: | Line 316: | ||
· PCR of comX-myc using Vent DNA Polymerase, PCR clean up<br> | · PCR of comX-myc using Vent DNA Polymerase, PCR clean up<br> | ||
· Digestion of pSB1C30-BBa_E0240 and pSB3K3-BBa_J04450 at XbaI and PstI<br> | · Digestion of pSB1C30-BBa_E0240 and pSB3K3-BBa_J04450 at XbaI and PstI<br> | ||
| - | · | + | · <br> |
| - | · | + | · <br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 362: | Line 340: | ||
· Submitted primers for Gibson assembly of pSB3K3-100bpPcombox<br> | · Submitted primers for Gibson assembly of pSB3K3-100bpPcombox<br> | ||
· Inoculation of pSB3K3-BBa_J04450<br> | · Inoculation of pSB3K3-BBa_J04450<br> | ||
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | + | ||
<br> <b> July 8</b> <br> | <br> <b> July 8</b> <br> | ||
| Line 375: | Line 352: | ||
· Gel purification of digested BBa_J04450<br> | · Gel purification of digested BBa_J04450<br> | ||
· Received pCEPcin from Universite de Toulouse, France<br> | · Received pCEPcin from Universite de Toulouse, France<br> | ||
| - | · | + | · <br> |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 391: | Line 364: | ||
· PCR of 100bp Pcombox using Phusion polymerase<br> | · PCR of 100bp Pcombox using Phusion polymerase<br> | ||
· Inoculation of pCEPcin<br> | · Inoculation of pCEPcin<br> | ||
| - | · | + | · <br> |
| - | + | ||
| - | + | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 1,393: | Line 1,364: | ||
<br><b>June 2</b> <br> | <br><b>June 2</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
| - | |||
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 1,603: | Line 1,573: | ||
<br> <b> July 7 </b> <br> | <br> <b> July 7 </b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
<br> <b> July 8 </b> <br> | <br> <b> July 8 </b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
<br> <b> July 9</b> <br> | <br> <b> July 9</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | + | · <br> | |
<br> <b> July 10</b> <br> | <br> <b> July 10</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | + | ||
| - | + | ||
| - | + | ||
<br> <b> July 11</b> <br> | <br> <b> July 11</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
</div> | </div> | ||
</div> | </div> | ||
| Line 1,661: | Line 1,611: | ||
<br> <b> July 14 </b> <br> | <br> <b> July 14 </b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
| - | + | · <br> | |
| - | + | · <br> | |
| - | + | · <br> | |
| - | + | · <br> | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
<br> <b> July 15 </b> <br> | <br> <b> July 15 </b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
<br> <b> July 16</b> <br> | <br> <b> July 16</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | + | ||
| - | + | ||
| - | + | ||
<br> <b> July 17</b> <br> | <br> <b> July 17</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
<br> <b> July 18</b> <br> | <br> <b> July 18</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | · | + | · <br> |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
</div> | </div> | ||
</div> | </div> | ||
| Line 1,731: | Line 1,649: | ||
<br> <b> July 21 </b> <br> | <br> <b> July 21 </b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
| - | · | + | · <br> |
| - | + | · <br> | |
| - | + | · <br> | |
| - | + | · <br> | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
<br> <b> July 22 </b> <br> | <br> <b> July 22 </b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
| - | · | + | · <br> |
| - | + | · <br> | |
| - | + | · <br> | |
| - | + | · <br> | |
<br> <b> July 23</b> <br> | <br> <b> July 23</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
| - | · | + | · <br> |
| - | + | · <br> | |
| - | + | · <br> | |
| - | + | · <br> | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
<br> <b> July 24</b> <br> | <br> <b> July 24</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
| - | · | + | · <br> |
| - | + | · <br> | |
| - | + | · <br> | |
| - | + | · <br> | |
| - | + | ||
<br> <b> July 25</b> <br> | <br> <b> July 25</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
| - | · | + | · <br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 2,195: | Line 2,093: | ||
</div> | </div> | ||
</div> | </div> | ||
| + | <footer> | ||
| + | <script> | ||
| + | $(document).ready(function(){ | ||
| + | |||
| + | //Check to see if the window is top if not then display button | ||
| + | $(window).scroll(function(){ | ||
| + | if ($(this).scrollTop() > 100) { | ||
| + | $('.scrollToTop').fadeIn(); | ||
| + | } else { | ||
| + | $('.scrollToTop').fadeOut(); | ||
| + | } | ||
| + | }); | ||
| + | |||
| + | //Click event to scroll to top | ||
| + | $('.scrollToTop').click(function(){ | ||
| + | $('html, body').animate({scrollTop : 0},800); | ||
| + | return false; | ||
| + | }); | ||
| + | |||
| + | }); | ||
| + | </script> | ||
| + | <style type= "text/css"> | ||
| + | .scrollToTop{ | ||
| + | width:45px; | ||
| + | height:45px; | ||
| + | border-radius:100px; | ||
| + | text-align:center; | ||
| + | font-weight: bold; | ||
| + | position:fixed; | ||
| + | top:600px; | ||
| + | right:20px; | ||
| + | display: none; | ||
| + | } | ||
| + | .scrollToTop:hover{ | ||
| + | text-decoration:none; | ||
| + | } | ||
| + | </style> | ||
| + | <a href="#" class="scrollToTop"> | ||
| + | <div class="scrollToTop"> | ||
| + | <img src="https://encrypted-tbn0.gstatic.com/images?q=tbn:ANd9GcRs8_1CHz0PqQvfOCYtGu_z8zOzbkrV4ppuMcXl05HpEycJHazt" width="45" height="45"/> | ||
| + | </div> | ||
| + | </a> | ||
| + | </footer> | ||
</body> | </body> | ||
</html> | </html> | ||
}} | }} | ||
Revision as of 17:58, 10 October 2014
Notebook
|
Throughout the course of our project, we have continuously recorded our daily work, such as wetlab activities and research findings. The notebook for Pneuumonsensor and Riboregulator are displayed separately. PneumosensorRiboregulator |
Home |
Pneumosensor |
Riboregulator |
Human Practice |
Team |
WetLab |
Achievement |
