Team:Aachen/Project/Measurement Device

From 2014.igem.org

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Revision as of 16:53, 10 October 2014

WatsOn

One part of our biosensor system Cellock Holmes are our agar chips containing our engineered sensing cells that fluoresce in the presence of the pathogen Pseudomonas aeruginosa. However, bacteria are small and the fluorescent signal cannot be seen with the naked eye. Thus the central question "What's on the chip?" remains. To answer this question we present our measurement device WatsOn.

WatsOn fulfills several tasks in our system:

  • incubate the sensing cells and the sampling chip
  • prevent escape of potentially sampled pathogens and our genetically engineered cells
  • illuminate the chip with the right excitation wavelength for GFP
  • take a photo of the chips
  • analyze the fluorescence signal
  • give feedback to the user about the presence or absence of P. aeruginosa


Measurarty

Measurements

Software Evaluation

Secret Ingredients

Build your own WatsOn

Table 1: Needed number of pieces, components and costs for building your own measurement device

number of pieces components costs [$]
1 filter slide (LEE Designer Edition: medium yellow 010)5.20
1 [http://www.dx.com/p/uno-r3-development-board-microcontroller-mega328p-atmega16u2-compat-for-arduino-blue-black-215600#.VDfoEhY2KBl arduino UNO R3]11.65
40jumper-wire cable2.99
340er male header18.97
3 40er female header3.29
1 [http://www.dx.com/p/arduino-dht11-digital-temperature-humidity-sensor-138531#.VDgN8RY2KBk circuit board]2.99
1 [http://www.newark.com/pro-signal/rp006/audio-video-cable-hdmi-1m-black/dp/96T7446 HDMI cable]1.87
17 port USB hub16.28
1[http://www.dx.com/p/dx-original-ultra-mini-usb-2-0-802-11n-b-g-150mbps-wi-fi-wlan-wireless-network-adapter-black-252716#.VDfq-hY2KBk WLAN USB stick]5.35
1USB mouse and keyboard12.50
1 [http://corporate.evonik.com/en/products/pages/default.aspx case acrylic glass XT 6mm~0.52]50.65
1spray paint for acrylic glass6.54
1 aluminum block 100x100x15 mm14.23
1raspberry model B29.95
1[http://www.newark.com/webapp/wcs/stores/servlet/Search?catalogId=15003&langId=-1&storeId=10194&st=raspberry%20camera&pageSize=25&showResults=true raspberry camera]25.00
1[http://www.mouser.de/ProductDetail/Olimex-Ltd/A13-TS7/?qs=sGAEpiMZZMvfpQN6QVmrfD4gEyBI%252bTfeuUbAz70fnsQ%3d 7” touchscreen display]24.19
1 [http://www.dx.com/p/arduino-dht11-digital-temperature-humidity-sensor-138531#.VDgN8RY2KBk digital temperature sensor DHT-22]7.50
1 [http://www.newark.com/caddock/mp2060-10-0-1/resistor-power-film-10-ohm-60w/dp/04K3517 peltier-element 12706 12V 60W]5.02
1power supply32.89
10 [http://www.newark.com/cree/c503b-bcs-cv0z0461/led-blue-t-1-3-4-5mm-4-1cd-480nm/dp/04R6675 superflux LEDs 480nm]2.10
1cupboard button1.24
-total280.09

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