Team:Hong Kong HKUST/wetlab/protocols

(Difference between revisions)

Revision as of 15:44, 10 October 2014

We have a variety of protocols that we followed for working on our project. The step-by-step procedures of each technique can be found here!

BD FACSAriaIII User Guide	Dephosphorylation	Ethanol Precipitation	Gibson Assembly
Ligation	Liquid Mediums and Agarose Plates	M9 Minimal Medium	Mutagenesis 1 (Multiple Sites)
Mutagenesis 2 (Single Sites)	Mutagenesis 3 (Single Sites)	Oligonucleotide Phosphorylation and Annealing	PCR (routine Taq)
PCR (Phusion)	PCR (Vent)	RPU Measurement	The Inoue Method for Preparation and Transformation of Competent E. Coli: "Ultra-Competent" Cells

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