Team:Heidelberg
From 2014.igem.org
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- | <h1 class="dark-grey-text" style="text-align: right;"><span style="font-size: 0.8em;">circular <span class="red-text">heat-stable</span></span><br> | + | <h1 class="dark-grey-text" style="text-align: right;"><span style="font-size: 0.8em;">circular <span class="red-text">heat-stable</span></span><br>DNMT1</h1> |
- | <p>Wouldn´t it be great to amplify DNA in a normal PCR | + | <p>Wouldn´t it be great to amplify DNA in a normal PCR maintaining the epigenetic information coded in methylation patterns?</p> |
- | <p>The problem: | + | <p>The problem: DNMT I, an enzyme which is responsible for the establishment and maintenance of the individual methylation pattern of different cell types, is not heat stable. |
- | + | For iGEM 2014 we therefore create a PCR 2.0 with heat-stable DNMT I by circularization. | |
- | + | </p> | |
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Revision as of 19:12, 9 October 2014