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Team:NCTU Formosa/Notebook/test3
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Cultivation of [Plac+B0032+PSB1K3*4] Kr (G3) | Cultivation of [Plac+B0032+PSB1K3*4] Kr (G3) | ||
PCR: [mGFP+B0015+PSB1K3*6] Kr | PCR: [mGFP+B0015+PSB1K3*6] Kr | ||
| + | </p> | ||
| + | |||
| + | <div class="single note"> | ||
| + | <p> | ||
| + | 6/15 | ||
| + | Mini-prep [Plac+B0032+PSB1K3*4] Kr (G3) | ||
| + | Digestion of [Plac+B0032+PSB1K3*4] Kr (ES) (G3) | ||
</p> | </p> | ||
<br></div> | <br></div> | ||
<div class="single note"> | <div class="single note"> | ||
| - | + | <p> | |
| + | 6/24 | ||
| + | Digestion of [J23101] Ar (ES) and [B0032] Ar (XP) (G1) | ||
| + | Ligation of [B0032+K592100+J61048] Ar (G1) | ||
| + | Electrophoresis of plasmid and digestion product of [K592100+J61048] Kr (XP), [J23101] Ar (SP) and [B0032] Ar (SP) (G1) | ||
| + | Digestion of [T7 polymerase +J61048]Kr(XP),[J61048+J61048]Cr(XP) (G2) | ||
| + | </p> | ||
| + | <p> | ||
| + | 6/27 | ||
| + | Digestion of [J61048+J61048] Cr (XP) (G2) | ||
| + | Electrophoresis of [RBS+BFP]kr(ES)(mini).[T7polymerase](ES)(mini).[T7 promoter](mini) | ||
| + | </p> | ||
| + | <p> | ||
| + | 6/28 | ||
| + | Transformation of mini:[B0032+BFP+J61048] Cr (G2) | ||
| + | </p> | ||
| + | |||
<br></div> | <br></div> | ||
<div class="single note"> | <div class="single note"> | ||
| - | + | <p> | |
| + | 6/29 | ||
| + | PCR:[B0032+BFP+J61048]Cr then | ||
| + | Electrophoresis of the PCR products (G2) | ||
| + | </p> | ||
<br></div> | <br></div> | ||
</div> | </div> | ||
<div class="clear"></div> | <div class="clear"></div> | ||
</div> | </div> | ||
Revision as of 15:10, 9 October 2014
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6/2 PCR: [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2) Cultivation of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2) Electrophoresis of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3]Kr and [B0032+BFP*3] Kr plasmid (G2)
6/3 PCR: [T7 promoter*5] Ar Kr, [B0032+BFP*5] Kr , [J61048+J61048*5] Cr and [T7polymerase+J61048*5] Kr (G2) Electrophoresis of PCR products (G2) Cultivation of [B0032+BFP*10] Kr, [J61048+J61048*10] Cr and [T7polymerase+J61048*5] Kr (G2)
6/5 Transform of ligation product [K592100+J61048+PSBIK3] Kr (G1) Ligation of [BFP+B0032] Kr and [T7polymerase+J61048] Kr (G2) Ligation of [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)
6/6 Mini-prep of [J61048+J61048*3] Cr and [T7 promoter*3]Ar Kr (G2) Digestion of [J61048+J61048*3] Cr (XP) and [T7 promoter*3]Ar Kr(ES) (G2) Transform of [B0032+BFP] Kr and [T7polymerase+J61048] Kr (G2) Transform of ligation product [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)
6/7 PCR: [PSB1K3*6] Kr, [K592100+J61048+PSB1K3*10] Kr,[J23101*8] Ar ,[B0032*8] Ar (G1) PCR: [mGFP+B0015+PSB1K3*6] Kr and [mGFP+J61048+PSB1K3*6] Kr (G3)
6/8 Electrophoresis of plasmid and digestion product of [J61048+J61048](XP), [T7 promoter](ES) PCR: [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2) Cultivation of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2) Electrophoresis of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2)
6/9 Transform of [B0023*2 ] Ar ,[J23101*2] Ar, [K592100+J61048*2] Kr and [PSB1K3*2] Kr (G1) Electrophoresis of plasmid and digestion product of [RBS+BFP]Kr (ES),[T7 polymerase+J61048]Kr(XP),[T7 promoter]Kr Cr (ES),[J61048+J61048]Cr(XP) (G2) Mini-prep of the plasmid and then digested the plasmid:[T7 polymerase+J61048]Kr(XP),[RBS+BFP]Kr(ES) (G2)
6/10 Electrophoresis of plasmid and digestion product of [K592100+J61048*2] Kr (XP), [PSB1K3*2] Kr (EP),[J23101*2] Ar (SP) and [B0023*2 ] Ar (SP) (G1)
6/11 Digestion of [mGFP] Ar (ES), [J61048] Ar (SP) ,[B0015] Ar (SP) (G3)
6/12 Ligation of [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3) PCR: [Plac+B0032+PSB1K3*12] Kr (G3)
6/13 Transform of ligation product [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3) Cultivation of [Plac+B0032+PSB1K3*4] Kr (G3)
6/14 Mini-prep of [Plac+B0032+PSB1K3*2] Kr (G3) Cultivation of [Plac+B0032+PSB1K3*4] Kr (G3) PCR: [mGFP+B0015+PSB1K3*6] Kr
6/15 Mini-prep [Plac+B0032+PSB1K3*4] Kr (G3) Digestion of [Plac+B0032+PSB1K3*4] Kr (ES) (G3)
6/24 Digestion of [J23101] Ar (ES) and [B0032] Ar (XP) (G1) Ligation of [B0032+K592100+J61048] Ar (G1) Electrophoresis of plasmid and digestion product of [K592100+J61048] Kr (XP), [J23101] Ar (SP) and [B0032] Ar (SP) (G1) Digestion of [T7 polymerase +J61048]Kr(XP),[J61048+J61048]Cr(XP) (G2)
6/27 Digestion of [J61048+J61048] Cr (XP) (G2) Electrophoresis of [RBS+BFP]kr(ES)(mini).[T7polymerase](ES)(mini).[T7 promoter](mini)
6/28 Transformation of mini:[B0032+BFP+J61048] Cr (G2)
6/29 PCR:[B0032+BFP+J61048]Cr then Electrophoresis of the PCR products (G2)
