Team:NCTU Formosa/Notebook/test3

From 2014.igem.org

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6/2
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PCR: [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2)
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Cultivation of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2)
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Electrophoresis of  [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3]Kr and [B0032+BFP*3] Kr plasmid (G2)
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</p><p>
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6/3
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PCR: [T7 promoter*5] Ar Kr, [B0032+BFP*5] Kr , [J61048+J61048*5] Cr and [T7polymerase+J61048*5]  Kr (G2)
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Electrophoresis of PCR products (G2)
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Cultivation of [B0032+BFP*10] Kr, [J61048+J61048*10] Cr and [T7polymerase+J61048*5] Kr (G2)
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</p><p>
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6/5
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Transform of ligation product [K592100+J61048+PSBIK3] Kr (G1)
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Ligation of [BFP+B0032] Kr and [T7polymerase+J61048] Kr (G2)
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Ligation of [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)
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</p><p>
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6/6
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Mini-prep of [J61048+J61048*3] Cr and [T7 promoter*3]Ar Kr (G2)
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Digestion of [J61048+J61048*3] Cr (XP) and [T7 promoter*3]Ar Kr(ES) (G2)
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Transform of [B0032+BFP] Kr and [T7polymerase+J61048] Kr (G2)
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Transform of ligation product [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)
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</p><p>
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6/7
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PCR: [PSB1K3*6] Kr, [K592100+J61048+PSB1K3*10] Kr,[J23101*8] Ar ,[B0032*8] Ar (G1)
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PCR: [mGFP+B0015+PSB1K3*6] Kr and [mGFP+J61048+PSB1K3*6] Kr (G3)
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</p>
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<p>
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6/8
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Electrophoresis of plasmid and digestion product of [J61048+J61048](XP), [T7 promoter](ES)
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PCR: [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr  (G2)
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Cultivation of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr  (G2)
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Electrophoresis of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr  (G2)
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</p>
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<p>
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6/9
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Transform of [B0023*2 ] Ar ,[J23101*2] Ar, [K592100+J61048*2] Kr and [PSB1K3*2] Kr (G1)
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Electrophoresis of plasmid and digestion product of [RBS+BFP]Kr (ES),[T7 polymerase+J61048]Kr(XP),[T7 promoter]Kr Cr (ES),[J61048+J61048]Cr(XP) (G2)
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Mini-prep of the plasmid and then digested the plasmid:[T7 polymerase+J61048]Kr(XP),[RBS+BFP]Kr(ES)  (G2)
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</p>
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<p>
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6/10
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Electrophoresis of plasmid and digestion product of [K592100+J61048*2] Kr (XP), [PSB1K3*2] Kr (EP),[J23101*2] Ar (SP) and [B0023*2 ] Ar (SP) (G1)
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</p>
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<p>
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6/11
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Digestion of [mGFP] Ar (ES), [J61048] Ar (SP) ,[B0015] Ar (SP) (G3)
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</p>
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<p>
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6/12
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Ligation of [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)
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PCR: [Plac+B0032+PSB1K3*12] Kr (G3)
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</p><p>
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6/13
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Transform of ligation product [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)
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Cultivation of [Plac+B0032+PSB1K3*4] Kr (G3)
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</p>
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<p>
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6/14
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Mini-prep of [Plac+B0032+PSB1K3*2] Kr (G3)
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Cultivation of [Plac+B0032+PSB1K3*4] Kr (G3)
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PCR: [mGFP+B0015+PSB1K3*6] Kr
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</p>
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<br></div>
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Revision as of 15:06, 9 October 2014

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6/2 PCR: [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2) Cultivation of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2) Electrophoresis of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3]Kr and [B0032+BFP*3] Kr plasmid (G2)

6/3 PCR: [T7 promoter*5] Ar Kr, [B0032+BFP*5] Kr , [J61048+J61048*5] Cr and [T7polymerase+J61048*5] Kr (G2) Electrophoresis of PCR products (G2) Cultivation of [B0032+BFP*10] Kr, [J61048+J61048*10] Cr and [T7polymerase+J61048*5] Kr (G2)

6/5 Transform of ligation product [K592100+J61048+PSBIK3] Kr (G1) Ligation of [BFP+B0032] Kr and [T7polymerase+J61048] Kr (G2) Ligation of [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)

6/6 Mini-prep of [J61048+J61048*3] Cr and [T7 promoter*3]Ar Kr (G2) Digestion of [J61048+J61048*3] Cr (XP) and [T7 promoter*3]Ar Kr(ES) (G2) Transform of [B0032+BFP] Kr and [T7polymerase+J61048] Kr (G2) Transform of ligation product [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)

6/7 PCR: [PSB1K3*6] Kr, [K592100+J61048+PSB1K3*10] Kr,[J23101*8] Ar ,[B0032*8] Ar (G1) PCR: [mGFP+B0015+PSB1K3*6] Kr and [mGFP+J61048+PSB1K3*6] Kr (G3)

6/8 Electrophoresis of plasmid and digestion product of [J61048+J61048](XP), [T7 promoter](ES) PCR: [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2) Cultivation of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2) Electrophoresis of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2)

6/9 Transform of [B0023*2 ] Ar ,[J23101*2] Ar, [K592100+J61048*2] Kr and [PSB1K3*2] Kr (G1) Electrophoresis of plasmid and digestion product of [RBS+BFP]Kr (ES),[T7 polymerase+J61048]Kr(XP),[T7 promoter]Kr Cr (ES),[J61048+J61048]Cr(XP) (G2) Mini-prep of the plasmid and then digested the plasmid:[T7 polymerase+J61048]Kr(XP),[RBS+BFP]Kr(ES) (G2)

6/10 Electrophoresis of plasmid and digestion product of [K592100+J61048*2] Kr (XP), [PSB1K3*2] Kr (EP),[J23101*2] Ar (SP) and [B0023*2 ] Ar (SP) (G1)

6/11 Digestion of [mGFP] Ar (ES), [J61048] Ar (SP) ,[B0015] Ar (SP) (G3)

6/12 Ligation of [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3) PCR: [Plac+B0032+PSB1K3*12] Kr (G3)

6/13 Transform of ligation product [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3) Cultivation of [Plac+B0032+PSB1K3*4] Kr (G3)

6/14 Mini-prep of [Plac+B0032+PSB1K3*2] Kr (G3) Cultivation of [Plac+B0032+PSB1K3*4] Kr (G3) PCR: [mGFP+B0015+PSB1K3*6] Kr


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