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Line 59: |
Line 59: |
| <div class="calendar-note"> | | <div class="calendar-note"> |
| <div class="single note"> | | <div class="single note"> |
- | <p>6/2
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- | PCR: [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2)
| |
- | Cultivation of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2)
| |
- | Electrophoresis of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3]Kr and [B0032+BFP*3] Kr plasmid (G2)
| |
- | </p>
| |
- | <p>
| |
- | 6/3
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- | PCR: [T7 promoter*5] Ar Kr, [B0032+BFP*5] Kr , [J61048+J61048*5] Cr and [T7polymerase+J61048*5] Kr (G2)
| |
- | Electrophoresis of PCR products (G2)
| |
- | Cultivation of [B0032+BFP*10] Kr, [J61048+J61048*10] Cr and [T7polymerase+J61048*5] Kr (G2)
| |
- | </p><br></div>
| |
- | <div class="single note">
| |
- | <p>6/5
| |
- | Transform of ligation product [K592100+J61048+PSBIK3] Kr (G1)
| |
- | Ligation of [BFP+B0032] Kr and [T7polymerase+J61048] Kr (G2)
| |
- | Ligation of [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)
| |
- | </p>
| |
- | <p>
| |
- | 6/6
| |
- | Mini-prep of [J61048+J61048*3] Cr and [T7 promoter*3]Ar Kr (G2)
| |
- | Digestion of [J61048+J61048*3] Cr (XP) and [T7 promoter*3]Ar Kr(ES) (G2)
| |
- | Transform of [B0032+BFP] Kr and [T7polymerase+J61048] Kr (G2)
| |
- | Transform of ligation product [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)
| |
- | </p>
| |
- | <p>
| |
- | 6/7
| |
- | PCR: [PSB1K3*6] Kr, [K592100+J61048+PSB1K3*10] Kr,[J23101*8] Ar ,[B0032*8] Ar (G1)
| |
- | PCR: [mGFP+B0015+PSB1K3*6] Kr and [mGFP+J61048+PSB1K3*6] Kr (G3)
| |
- | </p>
| |
- | <p>
| |
- | Electrophoresis of PCR products (G1)
| |
- | Cultivation of [PSB1K3*6] Kr, [K592100+J61048+PSB1K3*10] Kr,[J23101*8] Ar and [B0032*8] Ar (G1)
| |
| <br></div> | | <br></div> |
| + | 1 |
| <div class="single note"> | | <div class="single note"> |
- | | + | 2 |
- | </p><p>6/8
| + | |
- | Electrophoresis of plasmid and digestion product of [J61048+J61048](XP), [T7 promoter](ES)
| + | |
- | PCR: [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2)
| + | |
- | Cultivation of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2)
| + | |
- | Electrophoresis of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2)
| + | |
- | </p>
| + | |
- | <p>
| + | |
- | 6/9
| + | |
- | Transform of [B0023*2 ] Ar ,[J23101*2] Ar, [K592100+J61048*2] Kr and [PSB1K3*2] Kr (G1)
| + | |
- | Electrophoresis of plasmid and digestion product of [RBS+BFP]Kr (ES),[T7 polymerase+J61048]Kr(XP),[T7 promoter]Kr Cr (ES),[J61048+J61048]Cr(XP) (G2)
| + | |
- | Mini-prep of the plasmid and then digested the plasmid:[T7 polymerase+J61048]Kr(XP),[RBS+BFP]Kr(ES) (G2)
| + | |
- | </p><p>6/10
| + | |
- | Electrophoresis of plasmid and digestion product of [K592100+J61048*2] Kr (XP), [PSB1K3*2] Kr (EP),[J23101*2] Ar (SP) and [B0023*2 ] Ar (SP) (G1)
| + | |
- | </p>
| + | |
| <br></div> | | <br></div> |
| <div class="single note"> | | <div class="single note"> |
- | 1
| + | 3 |
| <br></div> | | <br></div> |
| <div class="single note"> | | <div class="single note"> |
- | 2
| + | 4 |
| <br></div> | | <br></div> |
| </div> | | </div> |
| <div class="clear"></div> | | <div class="clear"></div> |
| </div> | | </div> |