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Team:NCTU Formosa/Notebook/test3
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<div class="calendar-note"> | <div class="calendar-note"> | ||
| - | <div class="single note"> | + | <div class="single note"><p>6/2 |
| - | + | ||
| - | <p>6/2 | + | |
PCR: [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2) | PCR: [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2) | ||
Cultivation of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2) | Cultivation of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2) | ||
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Electrophoresis of PCR products (G2) | Electrophoresis of PCR products (G2) | ||
Cultivation of [B0032+BFP*10] Kr, [J61048+J61048*10] Cr and [T7polymerase+J61048*5] Kr (G2) | Cultivation of [B0032+BFP*10] Kr, [J61048+J61048*10] Cr and [T7polymerase+J61048*5] Kr (G2) | ||
| + | </p><br></div> | ||
| + | <div class="single note"> | ||
| + | <p>6/5 | ||
| + | Transform of ligation product [K592100+J61048+PSBIK3] Kr (G1) | ||
| + | Ligation of [BFP+B0032] Kr and [T7polymerase+J61048] Kr (G2) | ||
| + | Ligation of [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3) | ||
| + | </p> | ||
| + | <p> | ||
| + | 6/6 | ||
| + | Mini-prep of [J61048+J61048*3] Cr and [T7 promoter*3]Ar Kr (G2) | ||
| + | Digestion of [J61048+J61048*3] Cr (XP) and [T7 promoter*3]Ar Kr(ES) (G2) | ||
| + | Transform of [B0032+BFP] Kr and [T7polymerase+J61048] Kr (G2) | ||
| + | Transform of ligation product [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3) | ||
| + | </p> | ||
| + | <p> | ||
| + | 6/7 | ||
| + | PCR: [PSB1K3*6] Kr, [K592100+J61048+PSB1K3*10] Kr,[J23101*8] Ar ,[B0032*8] Ar (G1) | ||
| + | PCR: [mGFP+B0015+PSB1K3*6] Kr and [mGFP+J61048+PSB1K3*6] Kr (G3) | ||
| + | </p> | ||
| + | <p> | ||
| + | Electrophoresis of PCR products (G1) | ||
| + | Cultivation of [PSB1K3*6] Kr, [K592100+J61048+PSB1K3*10] Kr,[J23101*8] Ar and [B0032*8] Ar (G1) | ||
| + | </p><p>6/8 | ||
| + | Electrophoresis of plasmid and digestion product of [J61048+J61048](XP), [T7 promoter](ES) | ||
| + | PCR: [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2) | ||
| + | Cultivation of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2) | ||
| + | Electrophoresis of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2) | ||
| + | </p> | ||
| + | <p> | ||
| + | 6/9 | ||
| + | Transform of [B0023*2 ] Ar ,[J23101*2] Ar, [K592100+J61048*2] Kr and [PSB1K3*2] Kr (G1) | ||
| + | Electrophoresis of plasmid and digestion product of [RBS+BFP]Kr (ES),[T7 polymerase+J61048]Kr(XP),[T7 promoter]Kr Cr (ES),[J61048+J61048]Cr(XP) (G2) | ||
| + | Mini-prep of the plasmid and then digested the plasmid:[T7 polymerase+J61048]Kr(XP),[RBS+BFP]Kr(ES) (G2) | ||
| + | </p><p>6/10 | ||
| + | Electrophoresis of plasmid and digestion product of [K592100+J61048*2] Kr (XP), [PSB1K3*2] Kr (EP),[J23101*2] Ar (SP) and [B0023*2 ] Ar (SP) (G1) | ||
</p> | </p> | ||
| + | |||
<br></div> | <br></div> | ||
</div> | </div> | ||
<div class="clear"></div> | <div class="clear"></div> | ||
</div> | </div> | ||
Revision as of 12:56, 9 October 2014
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6/2 PCR: [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2) Cultivation of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2) Electrophoresis of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3]Kr and [B0032+BFP*3] Kr plasmid (G2)
6/3 PCR: [T7 promoter*5] Ar Kr, [B0032+BFP*5] Kr , [J61048+J61048*5] Cr and [T7polymerase+J61048*5] Kr (G2) Electrophoresis of PCR products (G2) Cultivation of [B0032+BFP*10] Kr, [J61048+J61048*10] Cr and [T7polymerase+J61048*5] Kr (G2)
6/5 Transform of ligation product [K592100+J61048+PSBIK3] Kr (G1) Ligation of [BFP+B0032] Kr and [T7polymerase+J61048] Kr (G2) Ligation of [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)
6/6 Mini-prep of [J61048+J61048*3] Cr and [T7 promoter*3]Ar Kr (G2) Digestion of [J61048+J61048*3] Cr (XP) and [T7 promoter*3]Ar Kr(ES) (G2) Transform of [B0032+BFP] Kr and [T7polymerase+J61048] Kr (G2) Transform of ligation product [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)
6/7 PCR: [PSB1K3*6] Kr, [K592100+J61048+PSB1K3*10] Kr,[J23101*8] Ar ,[B0032*8] Ar (G1) PCR: [mGFP+B0015+PSB1K3*6] Kr and [mGFP+J61048+PSB1K3*6] Kr (G3)
Electrophoresis of PCR products (G1) Cultivation of [PSB1K3*6] Kr, [K592100+J61048+PSB1K3*10] Kr,[J23101*8] Ar and [B0032*8] Ar (G1)
6/8 Electrophoresis of plasmid and digestion product of [J61048+J61048](XP), [T7 promoter](ES) PCR: [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2) Cultivation of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2) Electrophoresis of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2)
6/9 Transform of [B0023*2 ] Ar ,[J23101*2] Ar, [K592100+J61048*2] Kr and [PSB1K3*2] Kr (G1) Electrophoresis of plasmid and digestion product of [RBS+BFP]Kr (ES),[T7 polymerase+J61048]Kr(XP),[T7 promoter]Kr Cr (ES),[J61048+J61048]Cr(XP) (G2) Mini-prep of the plasmid and then digested the plasmid:[T7 polymerase+J61048]Kr(XP),[RBS+BFP]Kr(ES) (G2)
6/10 Electrophoresis of plasmid and digestion product of [K592100+J61048*2] Kr (XP), [PSB1K3*2] Kr (EP),[J23101*2] Ar (SP) and [B0023*2 ] Ar (SP) (G1)
