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Team:Hong Kong HKUST/wetlab/notebook
From 2014.igem.org
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| - | <div id="satu" align="center"> <h2>June | + | <div id="satu" align="center"> <h2>June 2014</h2> |
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<a href="#" class="head">Week 4</a> | <a href="#" class="head">Week 4</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br><b>June | + | <br><b>June 23</b><br> |
| - | + | Wetlab<br> | |
| + | · Transformation of BBa_B0015 from iGEM Kit Plate 2011 and BBa_K880005 <br> | ||
| + | · Primer design for PCR of comX-myc and comW-FLAG from genomic DNA of S. Pneumoniae NCTC 7465 strain<br> | ||
· <br> | · <br> | ||
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| + | <br><b>June 24</b> <br> | ||
| + | Wetlab<br> | ||
| + | · Inoculation of BBa_B0015<br> | ||
· <br> | · <br> | ||
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| - | <br><b>June | + | <br><b>June 25</b> <br> |
Wet lab<br> | Wet lab<br> | ||
| - | · <br> | + | · Digestion of BBa_B0015 plasmid extraction product with XbaI and PstI, gel extraction and gel purification<br> |
| - | · <br> | + | · Inoculation of BBa_K880005<br> |
· <br> | · <br> | ||
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| - | <br><b>June | + | <br><b>June 26</b> <br> |
Wet lab<br> | Wet lab<br> | ||
| - | · <br> | + | · Digestion of BBa_K880005 plasmid extraction product with SpeI and PstI, gel extraction and gel purification<br> |
· <br> | · <br> | ||
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| - | <br><b>June | + | <br><b>June 27</b> <br> |
Wet lab<br> | Wet lab<br> | ||
| - | · <br> | + | · Ligation of BBa_B0015 insert cut with XbaI and PstI into pSB1C3-K880005 backbone cut with SpeI and PstI as negative control<br> |
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<a href="#" class="head">Week 5</a> | <a href="#" class="head">Week 5</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br><b>June | + | <br><b>June 30</b> <br> |
Wet lab<br> | Wet lab<br> | ||
| - | · <br> | + | · Digestion of BBa_B0015 plasmid extraction product with XbaI and PstI, gel extraction and gel purification<br> |
| - | · <br> | + | · Gel check of purified BBa_B0015 insert<br> |
| - | · <br> | + | · Transformation of ligated pSB1C3-K880005.B0015<br> |
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Revision as of 13:48, 3 October 2014
Notebook
|
Throughout the course of our project, we have continuously recorded our daily work, such as wetlab activities and research findings. The notebook for Pneuumonsensor and Riboregulator are displayed separately. PneumosensorRiboregulator |
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