Team:Hong Kong HKUST/data

From 2014.igem.org

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<h2>Pneumosensor Data page</h2>
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<h2>Pneumosensor Parts</h2>
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<p>This page shows all the parts we have made or used in our whole project. Click on the links below to see our constructs on the Registry of Standard Biological Parts in detail. Click <a href= “https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/results”> here </a>to access our result page for an overview of our achievements with those parts.</p>
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<u>Our Favorite New Parts:</u>
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<u>Pre-existing Parts:</u>
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<u>We Also Characterized the Following Parts:</u>
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How our system works:
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[Diagram with Biobrick Code]
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<b>Data For Our Favorite New Parts:</b>
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<li> <b> Group 1/2???</b>
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<li> <b>&sigma;<sup>x</sup>, <a href= “http://parts.igem.org/Part:BBa_K1379004”> BBa_K1379004 </a> </b>: gene sequence of competence protein comX which produces &sigma;<sup>x</sup> factor. &sigma;<sup>x</sup> factor induces promoter containing combox sequence (P<sub>celA</sub> and P<sub>helicase</sub>).
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<li> <b>P<sub>celA</sub> promoter, <a href= “http://parts.igem.org/Part:BBa_K1379000”> BBa_K1379000 </a></b>: a &sigma;<sup>x</sup>-inducible promoter (containing combox sequence) which initiates the transcription of competence CelA protein.
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<li> <b>P<sub>helicase</sub> promoter, <a href= “http://parts.igem.org/Part:BBa_K1379001”> BBa_K1379001 </a> </b>: a &sigma;<sup>x</sup>-inducible promoter (containing combox sequence) which initiates the transcription of helicase protein.
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<b>Data for Pre-existing Parts:</b>
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<li> <b> Group 1/2???</b>
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<li><b>Strong promoter, strong RBS combination, <a href= “http://parts.igem.org/Part:BBa_K880005”> BBa_K880005 </a></b>(Team Michigan iGEM, 2012) : combination of strong constitutive promoter (<a href= “http://parts.igem.org/wiki/index.php?title=Part:BBa_J23100”> BBa_J23100</a>) and strong RBS (<a href= “http://parts.igem.org/wiki/index.php?title=Part:BBa_B0034”> BBa_B0034 </a>) was used as a backbone for a very high level of expression of ComX protein (&sigma;<sup>x</sup> factor).
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<li><b>GFP generator</b>, <a href= “http://parts.igem.org/Part:BBa_E0240”> BBa_E0240 </a> </b>(Antiquity Boston University iGEM, 2004) : green fluorescent protein reporter (<a href= “http://parts.igem.org/Part:BBa_E0240”> BBa_E0040 </a>) with RBS (<a href= “http://parts.igem.org/Part:BBa_B0032”> BBa_B0032</a>) and double terminator (<a href= “http://parts.igem.org/Part:BBa_B0015”>  BBa_B0015</a>) was used as a reporter for characterization of P<sub>celA</sub> and P<sub>helicase</sub> promoters.
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<b>We Also Built and Characterized the Following Parts</b>
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<li><b>Group1/2???</b>
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<li> <b>&sigma;<sup>x</sup> Generator, <a href= “http://parts.igem.org/Part:BBa_K1379006”> BBa_K1379006 </a> </b> : <i>comX</i> gene sequence was assembled with constitutive promoter, RBS and double terminator so ComX protein is produced constitutively.
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<li> <b> P<sub>celA</sub>-GFP Generator, <a href= “http://parts.igem.org/Part:BBa_K1379002”> BBa_K1379002 </a></b>: &sigma;<sup>x</sup>-inducible P<sub>celA</sub> promoter was assembled with GFP generator.
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<li> <b>P<sub>helicase</sub>-GFP Generator, <a href= “http://parts.igem.org/Part:BBa_K1379003”> BBa_K1379003</a> </b> : &sigma;<sup>x</sup>-inducible P<sub>helicase</sub> promoter was assembled with GFP generator.
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<li> <b> &sigma;<sup>x</sup> Generator, P<sub>celA</sub>-GFP Generator, <a href= “http://parts.igem.org/Part:BBa_K1379005”> BBa_K1379005 </a></b>: &sigma;<sup>x</sup>-generator was assembled with P<sub>celA</sub>-GFP generator. Following the assemblage of both constructs, GFP was successfully expressed.
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<li> <b>&sigma;<sup>x</sup> Generator, P<sub>helicase</suB>-GFP Generator, <a href= “http://parts.igem.org/Part:BBa_K1379007”> BBa_K1379007 </a> </b>: &sigma;<sup>x</sup>-generator was assembled with P<sub>helicase</sub>-GFP generator. Following the assemblage of both constructs, GFP was successfully expressed.
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Revision as of 11:47, 2 October 2014

Pneumosensor Parts

This page shows all the parts we have made or used in our whole project. Click on the links below to see our constructs on the Registry of Standard Biological Parts in detail. Click here to access our result page for an overview of our achievements with those parts.


How our system works: [Diagram with Biobrick Code]
Data For Our Favorite New Parts:

  • Group 1/2???
  • σx, BBa_K1379004 : gene sequence of competence protein comX which produces σx factor. σx factor induces promoter containing combox sequence (PcelA and Phelicase).
  • PcelA promoter, BBa_K1379000 : a σx-inducible promoter (containing combox sequence) which initiates the transcription of competence CelA protein.
  • Phelicase promoter, BBa_K1379001 : a σx-inducible promoter (containing combox sequence) which initiates the transcription of helicase protein.


Data for Pre-existing Parts:

  • Group 1/2???
  • Strong promoter, strong RBS combination, BBa_K880005 (Team Michigan iGEM, 2012) : combination of strong constitutive promoter ( BBa_J23100) and strong RBS ( BBa_B0034 ) was used as a backbone for a very high level of expression of ComX protein (σx factor).
  • GFP generator, BBa_E0240 (Antiquity Boston University iGEM, 2004) : green fluorescent protein reporter ( BBa_E0040 ) with RBS ( BBa_B0032) and double terminator ( BBa_B0015) was used as a reporter for characterization of PcelA and Phelicase promoters.


We Also Built and Characterized the Following Parts

  • Group1/2???
  • σx Generator, BBa_K1379006 : comX gene sequence was assembled with constitutive promoter, RBS and double terminator so ComX protein is produced constitutively.
  • PcelA-GFP Generator, BBa_K1379002 : σx-inducible PcelA promoter was assembled with GFP generator.
  • Phelicase-GFP Generator, BBa_K1379003 : σx-inducible Phelicase promoter was assembled with GFP generator.
  • σx Generator, PcelA-GFP Generator, BBa_K1379005 : σx-generator was assembled with PcelA-GFP generator. Following the assemblage of both constructs, GFP was successfully expressed.
  • σx Generator, Phelicase-GFP Generator, BBa_K1379007 : σx-generator was assembled with Phelicase-GFP generator. Following the assemblage of both constructs, GFP was successfully expressed.

Home

Pneumosensor

Riboregulator

Human Practice

Team

WetLab

Achievement

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