Team:KIT-Kyoto/Test/Notebook/Labnote

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<html>
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{{Team:KIT-Kyoto/logo}}
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<style>
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#contentSub, #footer-box, #catlinks,#search-controls, #p-logo, .printfooter, .firstHeading, .visualClear {display: none;}
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</style>
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<!--背景画像-->
 
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<div class="bgimg">
 
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<img src="https://static.igem.org/mediawiki/2014/4/43/KIT_pictures_hund.jpg" 　alt="" />
 
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<img src="https://static.igem.org/mediawiki/2014/7/7b/KIT_pictures_example.jpg" 　alt="" />
 
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<img src="https://static.igem.org/mediawiki/2014/b/b0/KIT_pictures_daityoukinn.jpg"  alt="" />
 
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</div>
 
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<!--背景画像終わり-->
 
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<!--サイドメニュー-->
 
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<div id="SideMenu">
 
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<div class="toplogo">
 
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<div>
 
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<img src="https://static.igem.org/mediawiki/2014/3/3b/KIT-Kyoto_Logo_MONO.png" width="250" height=140" alt="KIT Logo" /></a>
 
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</div>
 
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</div>
 
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<html>
<ul id="nav5">
<ul id="nav5">
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<li class="menuimg"><a href="/Team:KIT-Kyoto/Test"><img src="/wiki/images/c/c8/Kit_Home.png" width="400" height="27" /></a></li>
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        <li class="menuimg"><a href="#">HOME</a></li>
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<li class="menuimg"><a href="/Team:KIT-Kyoto/Test/About"><img src="/wiki/images/a/a1/Kit_About.png" width="400" height="27" /></a>
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<li class="menuimg"><a href="/Team:KIT-Kyoto/Test/About">ABOUT</a></li>
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<li class="menuimg"><a href="/Team:KIT-Kyoto/Test/Project"><img src="/wiki/images/f/ff/Kit_Project.png" width="400" height="27" /></a></li>
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<li class="menuimg"><a href="/Team:KIT-Kyoto/Test/Project">PROJECT</a></li>
</ul>
</ul>
<div class="active">
<div class="active">
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<a href="/Team:KIT-Kyoto/Notebook"><img src="/wiki/images/0/01/Kit_Notebook.png" width="400" height="27" /></a>
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<a href="/Team:KIT-Kyoto/Notebook">Notebook</a>
</div>
</div>
<ul class="submenu">
<ul class="submenu">
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<li class="btn"><a href="">October</a></li>
<li class="btn"><a href="">October</a></li>
</ul>
</ul>
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<li class="btn"><a href="">Protcol</a></li>
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<li class="btn"><a href="/Team:KIT-Kyoto/Notebook/Protocol">Protcol</a></li>
<li class="btn"><a href="">DesignNote</a></li>
<li class="btn"><a href="">DesignNote</a></li>
</ul>
</ul>
<ul id="nav5">
<ul id="nav5">
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<li class="menuimg"><a href="/Team:KIT-Kyoto/Test/Parts"><img src="/wiki/images/9/9e/Kit_Parts.png" width="400" height="27" /></a></li>
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<li class="menuimg"><a href="/Team:KIT-Kyoto/Test/Parts">PARTS</a></li>
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<li class="menuimg"><a href="/Team:KIT-Kyoto/Test/HumanPractice"><img src="/wiki/images/1/1b/Humanpractice.png" width="400" height="27" /></a></li>
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<li class="menuimg"><a href="/Team:KIT-Kyoto/HumanPractice">HUMAN PRACTICEs</a></li>
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<li class="menuimg"><a href="/Team:KIT-Kyoto/Test/Safety"><img src="/wiki/images/a/a2/Kit_Safety.png" width="400" height="27" /></a></li>
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<li class="menuimg"><a href="/Team:KIT-Kyoto/Test/Safety">SAFETY</a></li>
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<li class="menuimg"><a href="/Team:KIT-Kyoto/Test/Attributions"><img src="/wiki/images/0/08/Kit_Attributions.png" width="400" height="27" /></a></li>
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<li class="menuimg"><a href="/Team:KIT-Kyoto/Test/Attributions">ATTRIBUTIONS</a></li>
</ul>
</ul>
</div>
</div>
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<h2>June</h2>
<h2>June</h2>
<div class="scroll"></div>
<div class="scroll"></div>
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<h3>6/24</h3>
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<!-- タイトル -->
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<p class="sentence"><a href=""><img src="https://static.igem.org/mediawiki/2014/7/7f/Kitlogo.png" width="120" height="120" alt="kit" style="float:right;margin:20px;"></a>KIT, Kyoto Institute of Technology, is a national university in Kyoto, which had been the capital city of Japan for more than a thousand years. KIT was founded to do research on silk and textile, important for artistic kimono industries for Kyoto.
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<h3>June 10, 2014</h3>
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<!-- タイトル終わり -->
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Though its name has 'technology' in it, KIT is a fusion of science and arts. KIT has been committed in the genetics more recently. A good example is the Drosophila Genetic Resource Center (DGRC), which collects stocks of Drosophila, an essential model organism in life science research, from laboratories around the world. There are only two other such centers in the world.
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The institute has also collaborated actively with industries in Kyoto and beyond.
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<!-- 本文開始 -->
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<p class="sentence">
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KIT's competence is beyond science. It also has arts and design courses.
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<!-- 実験者名 -->
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OKAMOTO<br>
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KIT is a place of robust science and creative arts.  We will exert our strength
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<!-- 実験者名終わり -->
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As students of KIT, we are very proud of competing with wonderful students in other universities in iGEM. We will show you what you will get when arts and science merge. See you in Boston!
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<!--Miniprep-->
 +
<strong>Miniprep</strong>
 +
<table class="materials">
 +
<tr>
 +
<th rowspan="2">Host</th>
 +
<th colspan="2">Sample</th>
 +
</tr>
 +
<tr>
 +
<th>Vector</th>
 +
<th>Insert</th>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE1</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE2</TD>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS3</td>
 +
<tr>
 +
<td>BL21(DE3)</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS62</td>
 +
</tr>
 +
</table>
 +
<!--Miniprep終わり-->
 +
 
 +
<br>
 +
 
 +
<!--Restriction Digest-->
 +
<strong>Restriction Digest</strong>
 +
<table class="materials">
 +
<tr>
 +
<th rowspan="2">Host</th>
 +
<th colspan="2">Sample</th>
 +
<th rowspan="2">Enzyme</th>
 +
<th rowspan="2">Buffer</th>
 +
</tr>
 +
<tr>
 +
<th>Vector</th>
 +
<th>Insert</th>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE1</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE2</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS3</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE62</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE1</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE2</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS3</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE62</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
</table>
 +
<!--Ristriction Digest-->
 +
 
 +
<br>
</p>
</p>
 +
<!--本文終わり-->
 +
 +
<!-- タイトル -->
 +
<h3>June 10, 2014</h3>
 +
<!-- タイトル終わり -->
 +
 +
<!-- 本文開始 -->
 +
<p class="sentence">
 +
<!-- 実験者名 -->
 +
OKAMOTO<br>
 +
<!-- 実験者名終わり -->
 +
<!--Miniprep-->
 +
<strong>Miniprep</strong>
 +
<table class="materials">
 +
<tr>
 +
<th rowspan="2">Host</th>
 +
<th colspan="2">Sample</th>
 +
</tr>
 +
<tr>
 +
<th>Vector</th>
 +
<th>Insert</th>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE1</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE2</TD>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS3</td>
 +
<tr>
 +
<td>BL21(DE3)</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS62</td>
 +
</tr>
 +
</table>
 +
<!--Miniprep終わり-->
 +
 +
<br>
 +
 +
<!--Restriction Digest-->
 +
<strong>Restriction Digest</strong>
 +
<table class="materials">
 +
<tr>
 +
<th rowspan="2">Host</th>
 +
<th colspan="2">Sample</th>
 +
<th rowspan="2">Enzyme</th>
 +
<th rowspan="2">Buffer</th>
 +
</tr>
 +
<tr>
 +
<th>Vector</th>
 +
<th>Insert</th>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE1</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE2</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS3</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE62</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE1</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE2</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS3</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE62</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
</table>
 +
<!--Ristriction Digest-->
 +
 +
<br>
 +
</p>
 +
<!--本文終わり-->
 +
 +
 +
<h2>July</h2>
<h2>July</h2>
<div class="scroll"></div>
<div class="scroll"></div>
-
<h3>7/1</h3>
 
-
<p class="sentence">
 
-
<div class="btn03">
 
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
 
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</div>
 
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<div class="btn04">
 
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<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
 
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</div>
 
-
<div class="clear"><hr /></div>
 
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<div class="btn03">
 
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
 
-
</div>
 
-
<div class="btn04">
 
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
 
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</div>
 
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<div class="clear"><hr /></div>
 
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<h3>7/4</h3>
 
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<div class="btn03">
 
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<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
 
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</div>
 
-
<div class="btn04">
 
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
 
-
</div>
 
-
<div class="clear"><hr /></div>
 
-
<div class="btn03">
 
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
 
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</div>
 
-
<div class="btn04">
 
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
 
-
</div>
 
-
<div class="clear"><hr /></div>
 
-
<div class="btn03">
 
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
 
-
</div>
 
-
<div class="btn04">
 
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
 
-
</div>
 
-
<div class="clear"><hr /></div>
 
-
<div class="btn03">
 
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
 
-
</div>
 
-
<div class="btn04">
 
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
 
-
</div>
 
-
<div class="clear"><hr /></div>
 
-
<div class="btn03">
 
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
 
-
</div>
 
-
<div class="btn04">
 
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
 
-
</div>
 
-
<div class="clear"><hr /></div>
 
-
<br>
 
<h3>7/25</h3>
<h3>7/25</h3>
<br>
<br>
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               <div><a href="http://www.mediawiki.org/" style="float:left;margin:10px auto;"><img src="/wiki/skins/common/images/poweredby_mediawiki_88x31.png" height="31" width="88" alt="Powered by MediaWiki" /></a>
               </div>    
               </div>    
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              <div><a href="http://creativecommons.org/licenses/by/3.0/" style="float:right;margin:10px auto;"><img src="http://i.creativecommons.org/l/by/3.0/88x31.png" alt="Attribution 3.0 Unported" width="88" height="31" /></a>     
 +
              </div>
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<div id="kitfooter">       
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              <div><a href="http://creativecommons.org/licenses/by/3.0/" style="float:left;margin:10px auto;"><img src="http://i.creativecommons.org/l/by/3.0/88x31.png" alt="Attribution 3.0 Unported" width="88" height="31" /></a>     
 
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              </div>
 
     </div> <!-- close kitfooter-box -->
     </div> <!-- close kitfooter-box -->
</div>
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Latest revision as of 06:23, 2 October 2014

LabNote

June

June 10, 2014

OKAMOTO
Miniprep

Host Sample
Vector Insert
BL21(DE3) pGEX6P-2 CuMTSE1
BL21(DE3) pGEX6P-2 CuMTSE2
BL21(DE3) pGEX6P-2 CuMTS3
BL21(DE3) pGEX6P-2 CuMTS62

Restriction Digest
Host Sample Enzyme Buffer
Vector Insert
BL21(DE3)PlysS pGEX6P-2 CuMTSE1 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE2 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTS3 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE62 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE1 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE2 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTS3 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE62 Sal1, Not1 Buffer H

June 10, 2014

OKAMOTO
Miniprep

Host Sample
Vector Insert
BL21(DE3) pGEX6P-2 CuMTSE1
BL21(DE3) pGEX6P-2 CuMTSE2
BL21(DE3) pGEX6P-2 CuMTS3
BL21(DE3) pGEX6P-2 CuMTS62

Restriction Digest
Host Sample Enzyme Buffer
Vector Insert
BL21(DE3)PlysS pGEX6P-2 CuMTSE1 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE2 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTS3 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE62 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE1 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE2 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTS3 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE62 Sal1, Not1 Buffer H

July

7/25



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