Team:KIT-Kyoto/Test/Notebook/Labnote

From 2014.igem.org

(Difference between revisions)
 
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Line 27: Line 27:
<li class="btn"><a href="">October</a></li>
<li class="btn"><a href="">October</a></li>
</ul>
</ul>
-
<li class="btn"><a href="">Protcol</a></li>
+
<li class="btn"><a href="/Team:KIT-Kyoto/Notebook/Protocol">Protcol</a></li>
<li class="btn"><a href="">DesignNote</a></li>
<li class="btn"><a href="">DesignNote</a></li>
</ul>
</ul>
Line 45: Line 45:
<h2>June</h2>
<h2>June</h2>
<div class="scroll"></div>
<div class="scroll"></div>
 +
<!-- タイトル -->
<h3>June 10, 2014</h3>
<h3>June 10, 2014</h3>
 +
<!-- タイトル終わり -->
 +
 +
<!-- 本文開始 -->
<p class="sentence">
<p class="sentence">
-
OKAMOTO
+
<!-- 実験者名 -->
 +
OKAMOTO<br>
 +
<!-- 実験者名終わり -->
 +
<!--Miniprep-->
<strong>Miniprep</strong>
<strong>Miniprep</strong>
<table class="materials">
<table class="materials">
<tr>
<tr>
-
<td rowspan="2">Host</td>
+
<th rowspan="2">Host</th>
-
<td colspan="2">Sample</td>
+
<th colspan="2">Sample</th>
</tr>
</tr>
<tr>
<tr>
-
<td>Vector</td>
+
<th>Vector</th>
-
<td>Insert</td>
+
<th>Insert</th>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE1</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE2</TD>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS3</td>
 +
<tr>
 +
<td>BL21(DE3)</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS62</td>
</tr>
</tr>
</table>
</table>
 +
<!--Miniprep終わり-->
 +
 +
<br>
 +
 +
<!--Restriction Digest-->
 +
<strong>Restriction Digest</strong>
 +
<table class="materials">
 +
<tr>
 +
<th rowspan="2">Host</th>
 +
<th colspan="2">Sample</th>
 +
<th rowspan="2">Enzyme</th>
 +
<th rowspan="2">Buffer</th>
 +
</tr>
 +
<tr>
 +
<th>Vector</th>
 +
<th>Insert</th>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE1</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE2</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS3</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE62</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE1</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE2</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS3</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE62</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
</table>
 +
<!--Ristriction Digest-->
 +
 +
<br>
</p>
</p>
-
<h2>July</h2>
+
<!--本文終わり-->
-
<div class="scroll"></div>
+
 
-
<h3>7/1</h3>
+
<!-- タイトル -->
 +
<h3>June 10, 2014</h3>
 +
<!-- タイトル終わり -->
 +
 
 +
<!-- 本文開始 -->
<p class="sentence">
<p class="sentence">
-
<div class="btn03">
+
<!-- 実験者名 -->
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
OKAMOTO<br>
-
</div>
+
<!-- 実験者名終わり -->
-
<div class="btn04">
+
<!--Miniprep-->
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
<strong>Miniprep</strong>
-
</div>
+
<table class="materials">
-
<div class="clear"><hr /></div>  
+
<tr>
-
<div class="btn03">
+
<th rowspan="2">Host</th>
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
<th colspan="2">Sample</th>
-
</div>
+
</tr>
-
<div class="btn04">
+
<tr>
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
<th>Vector</th>
-
</div>
+
<th>Insert</th>
-
<div class="clear"><hr /></div>
+
</tr>
-
<h3>7/4</h3>  
+
<tr>
-
<div class="btn03">
+
<td>BL21(DE3)</td>
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
<td>pGEX6P-2</td>
-
</div>
+
<td>CuMTSE1</td>
-
<div class="btn04">
+
</tr>
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
<tr>
-
</div>
+
<td>BL21(DE3)</td>
-
<div class="clear"><hr /></div>  
+
<td>pGEX6P-2</td>
-
<div class="btn03">
+
<td>CuMTSE2</TD>
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
</tr>
-
</div>
+
<tr>
-
<div class="btn04">
+
<td>BL21(DE3)</td>
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
<td>pGEX6P-2</td>
-
</div>
+
<td>CuMTS3</td>
-
<div class="clear"><hr /></div>  
+
<tr>
-
<div class="btn03">
+
<td>BL21(DE3)</td>
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
<td>pGEX6P-2</td>
-
</div>
+
<td>CuMTS62</td>
-
<div class="btn04">
+
</tr>
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
</table>
-
</div>
+
<!--Miniprep終わり-->
-
<div class="clear"><hr /></div>  
+
 
-
<div class="btn03">
+
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
-
</div>
+
-
<div class="btn04">
+
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
-
</div>
+
-
<div class="clear"><hr /></div>  
+
-
<div class="btn03">
+
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
-
</div>
+
-
<div class="btn04">
+
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
-
</div>
+
-
<div class="clear"><hr /></div>
+
<br>
<br>
 +
 +
<!--Restriction Digest-->
 +
<strong>Restriction Digest</strong>
 +
<table class="materials">
 +
<tr>
 +
<th rowspan="2">Host</th>
 +
<th colspan="2">Sample</th>
 +
<th rowspan="2">Enzyme</th>
 +
<th rowspan="2">Buffer</th>
 +
</tr>
 +
<tr>
 +
<th>Vector</th>
 +
<th>Insert</th>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE1</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE2</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS3</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE62</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE1</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE2</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS3</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE62</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
</table>
 +
<!--Ristriction Digest-->
 +
 +
<br>
 +
</p>
 +
<!--本文終わり-->
 +
 +
 +
 +
<h2>July</h2>
 +
<div class="scroll"></div>
<h3>7/25</h3>
<h3>7/25</h3>
<br>
<br>

Latest revision as of 06:23, 2 October 2014

LabNote

June

June 10, 2014

OKAMOTO
Miniprep

Host Sample
Vector Insert
BL21(DE3) pGEX6P-2 CuMTSE1
BL21(DE3) pGEX6P-2 CuMTSE2
BL21(DE3) pGEX6P-2 CuMTS3
BL21(DE3) pGEX6P-2 CuMTS62

Restriction Digest
Host Sample Enzyme Buffer
Vector Insert
BL21(DE3)PlysS pGEX6P-2 CuMTSE1 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE2 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTS3 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE62 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE1 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE2 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTS3 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE62 Sal1, Not1 Buffer H

June 10, 2014

OKAMOTO
Miniprep

Host Sample
Vector Insert
BL21(DE3) pGEX6P-2 CuMTSE1
BL21(DE3) pGEX6P-2 CuMTSE2
BL21(DE3) pGEX6P-2 CuMTS3
BL21(DE3) pGEX6P-2 CuMTS62

Restriction Digest
Host Sample Enzyme Buffer
Vector Insert
BL21(DE3)PlysS pGEX6P-2 CuMTSE1 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE2 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTS3 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE62 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE1 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE2 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTS3 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE62 Sal1, Not1 Buffer H

July

7/25



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August




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September




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October




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