Team:UCL/Science/Sequences

From 2014.igem.org

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<p><strong>Note: format is a little horrendous, will change to all CAPS, and at a set width (70-75 char), and add "br" for all later.</strong></p>
<p><strong>Note: format is a little horrendous, will change to all CAPS, and at a set width (70-75 char), and add "br" for all later.</strong></p>
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<h3>BioBrick Designs for Our Genes</h3>
<h3>BioBrick Designs for Our Genes</h3>
     <h4><a name="AzoR" href="http://parts.igem.org/Part: ">[New] AzoR Part-only sequence (597/612bp) [Link, see DT]</a></h4>
     <h4><a name="AzoR" href="http://parts.igem.org/Part: ">[New] AzoR Part-only sequence (597/612bp) [Link, see DT]</a></h4>
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     <p class="widthCorrect">Sequence taken from <a href="http://www.ncbi.nlm.nih.gov/nuccore/26986745?report=fasta&from=3267527&to=3268138">Lisbon</a>.<br><font size="2"><code>ATGAAACTGTTGCACATCGATTCGAGCATCCTGGGCGACAATTCCGCCTCGCGTCAGCTGAGCCGTGAAG
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     <p class="widthCorrect">Sequence taken from <a href="http://www.ncbi.nlm.nih.gov/nuccore/26986745?report=fasta&from=3267527&to=3268138">Lisbon</a>.<br>
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TGGTCGAAGCCTGGAAGGCTGCAGACCCAAGCGTTGAAGTGGTTTATCGCGACCTGGCTGCCGATGCCAT
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    <font size="2">
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CGCCCACTTCTCCGCTGCCACCCTGGTCGCTGCAGGCACCCCTGAAGACGTACGCGACGCGGCCCAGGCT
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        <table class="table table-striped table-bordered">
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TTCGAAGCCAAGCTGAGCGCCGAGACGCTCGAAGAGTTCCTGGCTGCCGATGCCGTGGTGATTGGTGCGC
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            <tr>
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CCATGTACAACTTCACCGTGCCGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGAC
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                <td style="text-align:right;">
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CTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCG
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                <pre>1<br />100<br />199<br />298<br />397<br />496<br />595</pre>
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GGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCA
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                </td>
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TCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGC
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                <td style="text-align:left;">
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GATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGA
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                <pre>ATG AAA CTG TTG CAC ATC GAT TCG AGC ATC CTG GGC GAC AAT TCC GCC TCG CGT CAG CTG AGC CGT GAA GTG GTC GAA GCC TGG AAG GCT GCA GAC CCA <br />AGC GTT GAA GTG GTT TAT CGC GAC CTG GCT GCC GAT GCC ATC GCC CAC TTC TCC GCT GCC ACC CTG GTC GCT GCA GGC ACC CCT GAA GAC GTA CGC GAC <br />GCG GCC CAG GCT TTC GAA GCC AAG CTG AGC GCC GAG ACG CTC GAA GAG TTC CTG GCT GCC GAT GCC GTG GTG ATT GGT GCG CCC ATG TAC AAC TTC ACC <br />GTG CCG ACC CAG CTC AAA GCC TGG ATT GAC CGC GTA GCC GTC GCC GGC AAG ACC TTC CGC TAC ACC GAA GCC GGC CCG CAA GGC CTG TGC GGC AAC AAG <br />AAA GTG GTG CTG GTT TCC ACT GCG GGT GGC CTG CAC GCT GGC CAG CCG ACT GGC GCC GGG CAT GAA GAC TTC CTG AAA GTG TTC CTG GGC TTC ATC GGG <br />ATT ACT GAC CTG GAA ATC GTC CGG GCC CAC GGC CTG GCC TAT GGC CCG GAG CAG CGC AGC CAG GCG ATC GAT GCT GCA CAG GCG CAG ATT GCC AGC GAG <br />CTG TTT GCG GCT GCC TGA </pre>
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</code></font></p>
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            </td>
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            </tr>
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        </table>
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    </font></p>
     <br>
     <br>

Revision as of 22:31, 28 September 2014

Goodbye Azodye UCL iGEM 2014

Gene and BioBrick Part Sequences

Laboratory Team

Summary

For ease of reference, the sequences for (1) our BioBrick Gene Designs, (2) Plasmid / Vector Backbones, and (3) BioBricks that we have used from that iGEM Registry Distribution, have been provided below. Clicking on the Gene/Plasmid/BioBrick headers will take you to pages describing the part in more detail and the source of the sequences.

Note: format is a little horrendous, will change to all CAPS, and at a set width (70-75 char), and add "br" for all later.

BioBrick Designs for Our Genes

[New] AzoR Part-only sequence (597/612bp) [Link, see DT]

Sequence taken from Lisbon.

1
100
199
298
397
496
595
 
ATG AAA CTG TTG CAC ATC GAT TCG AGC ATC CTG GGC GAC AAT TCC GCC TCG CGT CAG CTG AGC CGT GAA GTG GTC GAA GCC TGG AAG GCT GCA GAC CCA 
AGC GTT GAA GTG GTT TAT CGC GAC CTG GCT GCC GAT GCC ATC GCC CAC TTC TCC GCT GCC ACC CTG GTC GCT GCA GGC ACC CCT GAA GAC GTA CGC GAC 
GCG GCC CAG GCT TTC GAA GCC AAG CTG AGC GCC GAG ACG CTC GAA GAG TTC CTG GCT GCC GAT GCC GTG GTG ATT GGT GCG CCC ATG TAC AAC TTC ACC 
GTG CCG ACC CAG CTC AAA GCC TGG ATT GAC CGC GTA GCC GTC GCC GGC AAG ACC TTC CGC TAC ACC GAA GCC GGC CCG CAA GGC CTG TGC GGC AAC AAG 
AAA GTG GTG CTG GTT TCC ACT GCG GGT GGC CTG CAC GCT GGC CAG CCG ACT GGC GCC GGG CAT GAA GAC TTC CTG AAA GTG TTC CTG GGC TTC ATC GGG 
ATT ACT GAC CTG GAA ATC GTC CGG GCC CAC GGC CTG GCC TAT GGC CCG GAG CAG CGC AGC CAG GCG ATC GAT GCT GCA CAG GCG CAG ATT GCC AGC GAG 
CTG TTT GCG GCT GCC TGA


[New] AzoR 1B6 (heat-stable AzoR mutant) Part-only sequence (597/612bp) [Link, see DT]

Sequence given from Lisbon group directly.
ATGAAACTGTTGCACATCGATTCGAGCATCCTGGGCGACAATTCCGCCTCGCGCCAGCTGAGCC GTGAAGTGGTCGAAGCCTGGAAGGCTGCAGACCCAAGCGTGGAAGTGGTTTATCGCGACCTGGCTGCCGA TCCCATCGCCCACTTCTCCGCTGCCACCCTGGTCGCTGCAGGCACCCCTGAAGACGTACGCGACGCGGCC CAGGCTTTCGAAGCCAAGCTGAGCGCCGAGACGCTCGAAGAGTTCCTGGCTGCCGATGGCGTGGTGATTG GTGCGCCCATGTACAACTTCACCGTGCCGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGG CAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGAGCGGCGACAAGAAAGTGGTGCTGGTTTCC ACTGCGGGTGGCCAGCATGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGCGTTCCTGG GCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGACTATGGCCCGGAGCAGCGCAG CCAGGCGATAGATGCTGCACGGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGA


[Proposed] CotA Part-only sequence (1733/1539bp) [Link, see DT]

Sequence given from Lisbon group directly.
atgacacttgaaaaatttgtggatgctctcccaatcccagatacactaaa gccagtacagcaatcaaaagaaaaaacatactacgaagtcaccatggagg aatgcactcatcagctccatcgcgatctccctccaacccgcctgtggggc tacaacggcttatttccgggaccgaccattgaggttaaaagaaatgaaaa cgtatatgtaaaatggatgaataaccttccttccacgcatttccttccga ttgatcacaccattcatcacagtgacagccagcatgaagagcccgaggta aagactgttgttcatttacacggcggcgtcacgccagatgatagtgacgg gtatccggaggcttggttttccaaagactttgaacaaacaggaccttatt tcaaaagagaggtttatcattatccaaaccagcagcgcggggctatattg tggtatcacgatcacgccatggcgctcaccaggctaaatgtctatgccgg acttgtcggtgcatatatcattcatgacccaaaggaaaaacgcttaaaac tgccttcagacgaatacgatgtgccgcttcttatcacagaccgcacgatc aatgaggatggttctttgttttatccgagcgcaccggaaaacccttctcc gtcactgcctaatccttcaatcgttccggctttttgcggagaaaccatac tcgtcaacgggaaggtatggccatacttggaagtcgagccaaggaaatac cgattccgtgtcatcaacgcctccaatacaagaacctataacctgtcact cgataatggcggagattttattcagattggttcagatggagggctcctgc cgcgatctgttaaactgaattctttcagccttgcgcctgctgaacgttac gatatcatcattgacttcacagcatatgaaggagaatcgatcattttggc aaacagcgcgggctgcggcggtgacgtcaatcctgaaacagatgcgaata tcatgcaattcagagtcacaaaaccattggcacaaaaagacgaaagcaga aagccgaagtacctcgcctcatacccttcggtacagcatgaaagaataca aaacatcagaacgttaaaactggcaggcacccaggacgaatacggcagac ccgtccttctgcttaataacaaacgctggcacgatcccgtcacagaaaca ccaaaagtcggcacaactgaaatatggtccattatcaacccgacacgcgg aacacatccgatccacctgcatctagtctccttccgtgtattagaccggc ggccgtttgatatcgcccgttatcaagaaagcggggaattgtcctatacc ggtccggctgtcccgccgccgccaagtgaaaagggctggaaagacaccat tcaagcgcatgcaggtgaagtcctgagaatcgcggcgacattcggtccgt acagcggacgatacgtatggcattgccatattctagagcatgaagactat gacatgatgagaccgatggatataactgatccccataaataa


[New] BsDyP Part-only sequence (1251bp) [Link, see DT]

Sequence given from Lisbon group directly.
atgagcgatgaacagaaaaagccagaacaaattcacagacgggacattttaaaatgggga gcgatggcgggggcagccgttgcgatcggtgccagcggtctcggcggtctcgctccgctt gttcagactgcggctaagccatcgaaaaaggatgaaaaagaggaggagcagattgttccg ttttacggaaagcatcaggccggaatcacaactgcccatcagacgtatgtctattttgct gcactggatgtaacagcaaaagacaaaagcgacatcattacgttatttagaaactggaca agtctcacacagatgctgacgtctggcaagaaaatgtcagcggagcagagaaatcagtat ctgccgccgcaggatacaggtgagtctgctgatttatccccttccaacttaacggtcaca ttcggattcgggcctggcttttttgaaaaagacggaaaggaccgcttcggactgaaaagc aaaaaaccgaagcacctagccgctcttccagcgatgcccaatgacaacctggatgaaaag cagggaggcggggacatctgcattcaagtgtgtgcagacgacgagcaagtggcgtttcac gcgctgcgtaacctgctaaatcaagcggtcggcacttgtgaggtccgttttgtgaacaaa ggctttttaagcggtggaaaaaatggtgaaacaccgcgcaacctcttcgggtttaaagat ggaacaggcaaccagagcacgaaggatgacacgttgatgaactcgatcgtgtggattcag tctggtgagcctgactggatgacgggcggcacctatatggcttttcggaaaatcaaaatg ttccttgaggtatgggaccgctcttccctcaaggaccaagaggataccttcggccgcaga aaaagctcgggggcgccttttgggcagaaaaaggaaacagatcccgtgaagctgaatcaa attccgtctaattcacacgtcagtctagcgaaatctactgggaaacaaattttgcgaaga gctttctcttacacagaaggacttgatccgaaaaccggctatatggatgcaggtctcctg tttatcagttttcaaaaaaatcccgacaatcagtttatcccgatgctgaaggctctttca gcaaaggatgcgttaaacgaatatacacaaacaatcggttctgctttatacgcatgccca ggcggctgcaaaaaaggagaatatattgcccagcgtttgctggaatcataa


[Proposed] PpDyP Part-only sequence (861/864bp) [Link, see DT]

Sequence taken from Lisbon.
ATGCCGTTCCAGCAAGGTCTGCTTGCCACCCCGGTGCCGGCCCACGCCCGTCACCTGTTCTTCACCCTGC AGTCGCCCGAGGCGTTGCCTGCCGCGCTGGATGCCTTGCTGCCGCAGGTCGATGGCAAACAGTTGCTGCT GGGCGTAGGCGCCCCCTTGGCCAAGGCCCTGGGCCGGGAAATACCAGGCCTGCGCCCGTTCCCGCTACTG GATGCCGCGGTTGAGAACCCCAGCACCCAACACGCCCTGTGGCTGTGGCTGCGTGGCGACGAACGTGGCG ACCTGCTGCTGCGCACCCAGGCCCTGGAGCAGGCGCTGGCACCCGCCCTGAGCCTGGCCGACAGCGTTGA TGGCTTCCTGCACCGGGGCGGCCATGACCTTACCGGTTATGAAGACGGCACCGAAAACCCGACAGACGAA GAGGCGGTGCAAGCGGCCATTGCCGCAGATGGCTCCAGCTTTGCCGCGTTCCAGCTGTGGAAGCACGACC TGCAATACTTCAAGTCGCTGCCCCAGGCAGACCAGGACAACATCATCGGCCGTCGCTTGAGCGATAACGA AGAACTCGACGACGCCCCCGCGTCCGCGCACGTCAAGCGCACCGCCCAGGAAAGCTTTGAACCCGAGGCG TTCATGGTCCGTCGCTCGGTATCCTGGGCCGACCAACGCGGCGCCGGCCTGGCTTTCGTCGCCCTGGGCA AGAGTTTCGAGGCATTCGAGGTGCAGCTGCGGCGCATGAGTGGCCTGGAAGACGGCATCATCGACGGGCT GTACCGCTTCAGCCGCCCGCTGACGGGGGGCTACTACTGGTGCCCGCCAATGAGCGAGACGGGTGTCGAT CTAAGCCCCTTGCTGCGGGCCTGA


[Improvement] LiP Part-only sequence (1116bp) [Link, see DT]

Sequence taken from the initial page; not currently showing our improvements.
atggcttttaaacaattattcgccgctatttccttggctttgtctttgtctgctgctaatgctgctgctgttattgaaaaaagagctacttgttccaacg gtaagactgttggtgatgcttgttcttgtgcttggtttgatgttttggacgacatccaacaaaacttgtttcatggtggtcaatgtggtgctgaagctca tgaatctatcagattggttttccatgactccattgctatttctccagctatggaagctcaaggtaaatttggtggaggtggtgctgatggttccattatg attttcgatgatatcgaaaccgccttccatccaaatattggtttggacgaaatcgtcaagttgcaaaagccatttgttcaaaagcacggtgttactccag gtgattttattgcttttgctggtgctgttgctttgtctaattgtccaggtgctccacaaatgaatttctttactggtagagcaccagctactcaaccagc tccagatggtttggttccagaaccatttcataccgttgaccaaatcatcaacagagttaatgacgccggtgaatttgacgaattggaattggtttggatg ttgtctgctcattctgttgctgctgttaatgatgttgatccaactgttcaaggtttgccatttgattctaccccaggtattttcgattcccaattcttcg ttgaaacccaattgagaggtactgcttttccaggttctggtggtaatcaaggtgaagttgaatctccattgccaggtgaaattagaatccaatccgatca taccattgccagagattcaagaactgcttgtgaatggcaatccttcgttaacaatcaatccaagttggttgacgacttccaattcattttcttggctttg actcaattgggtcaagatccaaatgctatgactgattgctccgatgttattccacaatctaaacctatcccaggtaacttgccattttcattttttccag ctggtaagaccatcaaggatgttgaacaagcttgtgctgaaactccatttccaactttgactactttgccaggtccagaaacttctgttcaaagaatacc accaccaccaggtgct


[Improvement] Laccase Part-only sequence (Xbp) [Link, see DT]

Sequence taken from the initial page; not currently showing our improvements.
ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgcaacgtcgtgatttcttaaaatattccgtcgcgctg ggtgtggcttcggctttgccgctgtggagccgcgcagtatttgcggcagaacgcccaacgttaccgatccctgatttgctcacgaccgatgcccgtaatc gcattcagttaactattggcgcaggccagtccacctttggcgggaaaactgcaactacctggggctataacggcaatctgctggggccggcggtgaaatt acagcgcggcaaagcggtaacggttgatatctacaaccaactgacggaagagacaacgttgcactggcacgggctggaagtaccgggtgaagtcgacggc ggcccgcagggaattattccgccaggtggcaagcgctcggtgacgttgaacgttgatcaacctgccgctacctgctggttccatccgcatcagcacggca aaaccgggcgacaggtggcgatggggctggctgggctggtggtgattgaagatgacgagatcctgaaattaatgctgccaaaacagtggggtatcgatga tgttccggtgatcgttcaggataagaaatttagcgccgacgggcagattgattatcaactggatgtgatgaccgccgccgtgggctggtttggcgatacg ttgctgaccaacggtgcaatctacccgcaacacgctgccccgcgtggttggctgcgcctgcgtttgctcaatggctgtaatgcccgttcgctcaatttcg ccaccagcgacaatcgcccgctgtatgtgattgccagcgacggtggtctgctacctgaaccagtgaaggtgagcgaactgccggtgctgatgggcgagcg ttttgaagtgctggtggaggttaacgataacaaaccctttgacctggtgacgctgccggtcagccagatggggatggcgattgcgccgtttgataagcct catccggtaatgcggattcagccgattgctattagtgcctccggtgctttgccagacacattaagtagcctgcctgcgttaccttcgctggaagggctga cggtacgcaagctgcaactctctatggacccgatgctcgatatgatggggatgcagatgctaatggagaaatatggcgatcaggcgatggccgggatgga tcacagccagatgatgggccatatggggcacggcaatatgaatcatatgaaccacggcgggaagttcgatttccaccatgccaacaaaatcaacggtcag gcgtttgatatgaacaagccgatgtttgcggcggcgaaagggcaatacgaacgttgggttatctctggcgtgggcgacatgatgctgcatccgttccata tccacggcacgcagttccgtatcttgtcagaaaatggcaaaccgccagcggctcatcgcgcgggctggaaagataccgttaaggtagaaggtaatgtcag cgaagtgctggtgaagtttaatcacgatgcaccgaaagaacatgcttatatggcgcactgccatctgctggagcatgaagatacggggatgatgttaggg tttacggtataa


[New] ispB Part-only sequence (972bp) [Link, see EG]

Sequence shows the ispB gene, but we use the Reverse Complement to design primers to produce an antisense fragment for gene knockdown (i.e. as RNAi).ATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGTGT TAATGCGGCAATCCTTGAGCAGCTTAATTCCGACGTCCAACTGATCAATC AGTTAGGCTATTACATCGTCAGCGGCGGCGGTAAACGTATTCGTCCGATG ATTGCTGTACTGGCTGCACGAGCTGTTGGCTATGAGGGAAATGCGCATGT CACCATTGCTGCCCTGATCGAGTTTATCCACACGGCGACTCTGCTACACG ACGACGTTGTGGATGAATCAGATATGCGCAGGGGTAAAGCTACCGCCAAC GCCGCATTTGGCAATGCCGCCAGCGTGCTGGTAGGCGATTTTATTTATAC CCGCGCTTTCCAGATGATGACCAGCCTCGGTTCGCTCAAAGTGCTGGAAG TCATGTCAGAAGCCGTAAACGTCATCGCAGAAGGTGAAGTTCTGCAACTG ATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTAT CTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGA TTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGG CGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAA TGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAG GTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCAGAA CAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCT TCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGA CGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAG GTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACAT CGCTGTTCAACGCGATCGTTAA


[New] ispB Reverse Complement Part-only sequence (972bp) [Link, see DT]

TTAACGATCGCGTTGAACAGCGATGTGCGCGAGGCCGATGAGTGCTTCTC GCCAAGGGGTGTCCGGGAGCACCTGTAACGCTGCGATGGCTTTGTCTGCT TCTTCCTCGGCACGCTGACGCGTCCATTCAAGAGATCCACAAGCGTTCAT TGCTTCCAGAACCGGTTCCAGAAGATGGCGACCGTTACCCTGTTCGATGG CGGTACGGATCATCTGTGCCTGTTCTGGTGTGCCATGATGCATCGCATGC AGCAGCGGCAGCGTCGGTTTACCTTCGTTCAGATCGTCGCCGACATTTTT ACCTAACTGTTCGCCATCGGCATTGTAATCGAGTAAATCGTCGATCAACT GGAAAGCAGTGCCGAGATAGCGCCCATAATCCTGCAGGCCTTTCTCCTCC TCCGGCGTACAGCCAGCCAGAATCCCGGAACACTGCGCGGCAGCCTCAAA CAGACGCGCGGTTTTGCTATAGATAACGCGCATGTAGTTTTCTTCAGTGA TGTCCGGATCGTTAACGTTCATCAGTTGCAGAACTTCACCTTCTGCGATG ACGTTTACGGCTTCTGACATGACTTCCAGCACTTTGAGCGAACCGAGGCT GGTCATCATCTGGAAAGCGCGGGTATAAATAAAATCGCCTACCAGCACGC TGGCGGCATTGCCAAATGCGGCGTTGGCGGTAGCTTTACCCCTGCGCATA TCTGATTCATCCACAACGTCGTCGTGTAGCAGAGTCGCCGTGTGGATAAA CTCGATCAGGGCAGCAATGGTGACATGCGCATTTCCCTCATAGCCAACAG CTCGTGCAGCCAGTACAGCAATCATCGGACGAATACGTTTACCGCCGCCG CTGACGATGTAATAGCCTAACTGATTGATCAGTTGGACGTCGGAATTAAG CTGCTCAAGGATTGCCGCATTAACACCCGCCATATCTTGCGCGGTTAACT CATTGATTTTTTCTAAATTCAT


Plasmid Backbone / Vectors

pET-21a (+) sequence (Xbp) [Link, see DT]

atccggatatagttcctcctttcagcaaaaaacccctcaagacccgtttagaggccccaaggggttatgctagttattgc tcagcggtggcagcagccaactcagcttcctttcgggctttgttagcagccggatctcagtggtggtggtggtggtgctc gagtgcggccgcaagcttgtcgacggagctcgaattcggatccgcgacccatttgctgtccaccagtcatgctagccata tgtatatctccttcttaaagttaaacaaaattatttctagaggggaattgttatccgctcacaattcccctatagtgagt cgtattaatttcgcgggatcgagatctcgatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcg gttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcgg cgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcgg tgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgagatcccggacac catcgaatggcgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcagggtggtgaatgtgaaac cagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccac gtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaact ggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcga ttaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcg gcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgt ggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctccc atgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccatta agttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacg ggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgc tggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcg gtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgct ggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcac tggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctg gcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtaagttagctcactcattaggcaccgg gatctcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgca cttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctt tcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactg gtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccccacgggtgcgcatgatcgtgctc ctgtcgttgaggacccggctaggctggcggggttgccttactggttagcagaatgaatcaccgatacgcgagcgaacgtg aagcgactgctgctgcaaaacgtctgcgacctgagcaacaacatgaatggtcttcggtttccgtgtttcgtaaagtctgg aaacgcggaagtcagcgccctgcaccattatgttccggatctgcatcgcaggatgctgctggctaccctgtggaacacct acatctgtattaacgaagcgctggcattgaccctgagtgatttttctctggtcccgccgcatccataccgccagttgttt accctcacaacgttccagtaaccgggcatgttcatcatcagtaacccgtatcgtgagcatcctctctcgtttcatcggta tcattacccccatgaacagaaatcccccttacacggaggcatcagtgaccaaacaggaaaaaaccgcccttaacatggcc cgctttatcagaagccagacattaacgcttctggagaaactcaacgagctggacgcggatgaacaggcagacatctgtga atcgcttcacgaccacgctgatgagctttaccgcagctgcctcgcgcgtttcggtgatgacggtgaaaacctctgacaca tgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggt gttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcggagtgtatactggcttaactatgcggcatca gagcagattgtactgagagtgcaccatatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcagg cgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggc ggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaacc gtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcag aggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgac cctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatc tcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatcc ggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcag agcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggta tctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagc ggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggg gtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatcc ttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatc agtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgat acgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaa taaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgc cgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctgcaggcatcgtggtgtcacg ctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaa aagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagca ctgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgaga atagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaag tgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaaccc actcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgc cgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatc agggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccc cgaaaagtgccacctgaaattgtaaacgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttt taaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagttt ggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccacta cgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccg atttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgc tggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcg cca


pSB1C3 Part-only sequence (2070bp)

tactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggct tcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggata acgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacga gcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcct gttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgt aggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaag acacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactac ggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccg ctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctca gtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatc taaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagat ggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaatt cattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcc catggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattc tcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcac tccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacg aaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaata tccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatc cagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagtt ggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggca gaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagag


pSB1A3 Part-only sequence (2155bp)

tactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggct tcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggata acgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacga gcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcct gttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgt aggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaag acacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactac ggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccg ctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctca gtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatc taaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcct gactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagattt atcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctaga gtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccg gttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgc agtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtca ttctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattg gaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttt tactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttc ctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgca catttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaa aaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagag


pSB1K3 Part-only sequence (2204bp)

tactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggct tcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggata acgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacga gcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcct gttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgt aggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaag acacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactac ggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccg ctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctca gtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatc taaagtatatatgagtaaacttggtctgacagctcgagtcccgtcaagtcagcgtaatgctctgccagtgttacaaccaattaaccaattctgattagaa aaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactca ccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataa ggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagcttatgcatttctttccagacttgttcaacaggccagccatt acgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaatta caaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttt tcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtct gaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgca cctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctggagcaagacgtttcccgttgaa tatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagatt ttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcagctcgagtgccacctgacgtctaagaaaccattattatcatga cattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttct agag


BioBricks from iGEM Parts Registry Distributions

[Test use] J04450 Part-only sequence (1069bp)

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaat taatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca tactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtca cgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgg gacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttca aatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcg tggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaa ggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtg cttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgc ttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata


[Used] K314103 Part-only sequence (1638bp)

acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgcttt cttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgac cccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtg gactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatga gctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagaggtggtgaatgtgaaaccagtaacgttatacgatgtcg cagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggc ggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccg tcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcgg cggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgt tccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgca ttgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaa ttcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgct ggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgatacc gaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagg gccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggc cgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgatactagagaattgtgagcggataacaattgacattgtgagcggat aacaagatactgagcacatactagagaaagaggagaaa


[Test use] R0010 Part-only sequence (200bp)

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaat taatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca


[Not used] R0011 Part-only sequence (55bp)

aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca


[Not used] K206000 Part-only sequence (130bp)

acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctac tgtttctccataccgtttttttgggctagc


[Test use] B0034 Part-only sequence (12bp)

aaagaggagaaa


[Test use] K518012 Part-only sequence (828bp)

tcacacaggaaagtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttc gaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcc tgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatggga acgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtacc aacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaa tcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaa aaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa cgctgatagtgctagtgtagatcgctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagcca gattattaatccggcttttttattattt


[Not used] B0012 Part-only sequence (41bp)

tcacactggctcaccttcgggtgggcctttctgcgtttata


Full table

No. ID Name / Function Source State / Concentration / Date Made Gene Size / Sequence Initial Plasmid / Vector Comments
1 pAzoR / BBa_K1336000 FMN-dependent NADH-azoreductase 1 Pseudomonas putida Miniprep,
48 ng/uL,
15/07/17, TO. 		597 bp [Check! Not 612 bp?],
Genomic Sequence 		Expression vector pET-21a (+) (ampicillin resistant (ampR)), initially cloned between NdeI and BamHI. Plasmid provided by Lisbon; with Paper.
2 p1B6 (AzoR 1B6) / BBa_K1336001 Mutant: Heat-stable; FMN-dependent NADH-azoreductase 1 Pseudomonas putida Miniprep,
68 ng/uL,
15/07/14, TO.		 597 bp [Check! Not 612 bp?],
Genomic Sequence[Add sequence! Sent from Lisbon, see DT] 		Expression vector pET-21a (+) (ampR), initially cloned between NdeI and BamHI. Plasmid provided by Lisbon; with Paper.
3 pCotA / BBa_K1336002 Spore Coat Protein Laccase Bacillus subtilis Miniprep,
103 ng/uL,
15/07/14, TO. 		1733 bp [Check! Not 1539 bp?]
Genomic Sequence[Add sequence! Sent from Lisbon, see DT] 		Expression vector pET-21a (+) (ampR), initially cloned between NheI and BamHI. Plasmid provided by Lisbon; with Paper.
4 LiP / BBa_K1336003 Lignin Peroxidase Phanerochaete chrysosporium (White-Rot Fungi) [Being synthesised by Gene Oracle],
X ng/uL,
dd/mm/yy, Gene Oracle. 		X bp,
Genomic Sequence 		[Cloned directly into expression vector, pSB1C3, between EcoRI and PstI.] Synthesised (for free) by Gene Oracle. Sequence from Paper.
5 pBsDyP / BBa_K1336004 Dye Decolourising Peroxidase BSU38260 Bacillus subtilis Miniprep,
51 ng/uL,
15/07/14, TO.		 1251 bp,
Genomic Sequence[Add sequence! Sent from Lisbon, see DT] 		Expression vector pET-21a (+) (ampR), initially cloned between NdeI and BamHI. Plasmid provided by Lisbon; with Paper.
6 pPpDyP / BBa_K1336005 Dye Decolourising Peroxidase PP_3248 Pseudomonas putida Miniprep,
55 ng/uL,
15/07/14, TO. 		861 bp [Check! Not 864 bp?],
Genomic Sequence[Add sequence! Sent from Lisbon, see DT] 		Expression vector pET-21a (+) (ampR), initially cloned between NdeI and BamHI. Plasmid provided by Lisbon; with Paper.
7 BBa_J04450 RFP Coding Device Spring 2014 BioBrick Distribution.
Plate 4, Well 4B. [Check! DT?]. 		(1) Miniprep,
333 ng/uL,
01/07/14, TO.

(2) Miniprep,
38 ng/uL (NanoDrop, dodgy!)
01/07/14, TO. 		1069 bp,
Genomic Sequence[Add sequence! Made from combined BioBricks?] 		Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR). LacI-, and CAP-, sensitive; can fail if system contains LacI or CAP protein!
RFP Coding Device contains: LacI (R0010), strong RBS (B0034), mRFP1 (E1010), and double terminator (B0015 = B0010+B0012).
8 BBa_R0010 Promoter (LacI regulated) Spring 2014 BioBrick Distribution.
Plate 3, Well 4G. [Check! DT?]. 		Miniprep,
329.1 ng/uL,
01/07/14, TO. 		200 bp,
Genomic Sequence[Add sequence!] 		Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR).
Plasmid / Vector Map. 		This part is an inverting regulator sensitive to LacI and CAP. In the absence of LacI and CAP proteins, this part promotes transcription; in their presence, the part inhibits transcription. LacI can be inhibited by IPTG.
9 BBa_R0011 Promoter (LacI regulated, lambda pL hybrid) Spring 2014 BioBrick Distribution.
Plate 2, Well 6D (Inconsistent sequencing!). [Check! DT? / Maybe use Spring 2013 Distribution, Plate 5, Well 6G.]. 		Miniprep,
38 ng/uL (NanoDrop, dodgy!),
01/07/14, TO. 		55 bp,
Genomic Sequence[Add sequence!] 		Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR).
Plasmid / Vector Map. 		Inverting regulatory region controlled by LacI (BBa_C0010, BBa_C0012, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1.
10 BBa_K314103 Lac induced expression cassette Spring 2014 BioBrick Distribution.
Plate 1, Well 4D. [Check! DT?]. 		Miniprep,
334 ng/uL,
01/07/14, TO. 		1638 bp,
Genomic Sequence[Add sequence!] 		Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR).
Plasmid / Vector Map. 		Lactose (IPTG) inducible protein expression insert includes f1 origin (K314110), a Lac I generator (K314111), a lactose inducible promoter (R0011), and the Elowitz standard RBS (B0034).
11 BBa_K206000 pBAD Strong Promoter Spring 2014 BioBrick Distribution.
Plate 3, Well 14A. [Check! DT?]. 		Miniprep,
144 ng/uL,
01/07/14, TO. 		130 bp,
Genomic Sequence[Add sequence!] 		Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR).
Plasmid / Vector Map 		pBAD is an E. coli promoter that is induced by L-arabinose. In the absence of arabinose, the repressor protein AraC (BBa_I13458) binds to the AraI1 operator site of pBAD and the upstream operator site AraO2, blocking transcription; in its presence, transcription is permitted.
12 BBa_B0034 RBS Spring 2014 BioBrick Distribution.
Plate 4, Well 1N. [Check! DT?]. 		Miniprep,
156.5 ng/uL,
01/07/14, TO. 		12 bp,
Genomic Sequence[Add sequence!] 		Plasmid Backbone: pSB1A2, i.e. ampicillin resistant (ampR).
Plasmid / Vector Map 		RBS based on Elowitz (1999) repressilator.
13 BBa_K518012 RBS + RFP + double Terminator Spring 2014 BioBrick Distribution.
Plate 1, Well 18C. [Check! DT?]. 		(1) Miniprep,
49 ng/uL,
01/07/14, TO.

(2) Miniprep,
219.2 ng/uL,
08/08/14, YKH. 		828 bp,
Genomic Sequence[Add sequence!] 		Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR).
Plasmid / Vector Map 		This Coding Device contains: RBS.3 (medium) (B0032), mRFP1 (E1010), and double terminator (B0014 = B0012+B0011).
14 [CHECK: BAD PART !]
BBa_B0012 (2) 		Transcription Terminator for E. coli RNA polymerase Spring 2014 BioBrick Distribution.
Plate 2, Well 2B. [Check! DT?]. 		Miniprep,
128 ng/uL,
01/07/14, TO. 		41 bp,
Genomic Sequence[Add sequence!] 		Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR).
Plasmid / Vector Map 		TE from coliphage T7. This is a bad terminator (Experience: Fails). It is a promoter in the reverse direction.
# ID Name / Function Source State / Concentration / Date Made Gene Size / Sequence Initial Plasmid / Vector Comments

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