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Team:Bielefeld-CeBiTec/Notebook/Journal/rMFC/Jun
From 2014.igem.org
(Difference between revisions)
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<div class="content" style="margin-right:10%; margin-left:10%"> | <div class="content" style="margin-right:10%; margin-left:10%"> | ||
<ul> | <ul> | ||
| - | <li><b | + | <li><b>Initial cultivation experiments with neutral red </b> |
<ul> | <ul> | ||
<li> Cultivation of the <i>E. coli</i> strain with porines (attempt 1) and wildtyp as control (attempt 2) in LB-medium with 100 µM neutral red </li> | <li> Cultivation of the <i>E. coli</i> strain with porines (attempt 1) and wildtyp as control (attempt 2) in LB-medium with 100 µM neutral red </li> | ||
| Line 87: | Line 87: | ||
<li> Results </li> | <li> Results </li> | ||
<ul> | <ul> | ||
| - | <li> Attempt 1 turned red ( | + | <li> Attempt 1 turned red (pH = 7.495)</li> |
| - | <li> Attempt 2 remained yellow | + | <li> Attempt 2 remained yellow </li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
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</div> | </div> | ||
<div class="content" style="margin-right:10%; margin-left:10%"> | <div class="content" style="margin-right:10%; margin-left:10%"> | ||
| + | <ul> | ||
| + | <li><b> Reduction of neutral red </b> | ||
| + | <ul> | ||
| + | <li> Reducing agent: sodium dithionite </li> | ||
| + | <ul> | ||
| + | <li> Red solution got reduced and turned colorless </li> | ||
| + | <li> Got oxidised by air again afterwards </li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | <ul> | ||
| + | <li> Oxidizing agent: hydrogen peroxide </li> | ||
| + | <ul> | ||
| + | <li> Red solution stayed red </li> | ||
| + | <li> Yellow solution stayed yellow </li> | ||
| + | </ul> | ||
| + | <li> Conclusion: the color of the solution depends on the pH </li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | |||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 17:21, 26 September 2014
June |
 |
- Initial cultivation experiments with neutral red
- Cultivation of the E. coli strain with porines (attempt 1) and wildtyp as control (attempt 2) in LB-medium with 100 µM neutral red
- pH was set up at 8.75
- Results
- Attempt 1 turned red (pH = 7.495)
- Attempt 2 remained yellow
- Reduction of neutral red
- Reducing agent: sodium dithionite
- Red solution got reduced and turned colorless
- Got oxidised by air again afterwards
- Oxidizing agent: hydrogen peroxide
- Red solution stayed red
- Yellow solution stayed yellow
- Conclusion: the color of the solution depends on the pH
