Team:CityU HK/notebook/safety
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Revision as of 13:54, 23 September 2014
Safety
The organisms we used for this project :
Species |
Strain no. / name |
Risk Group |
Any risk to humans? |
E. coli K 12 |
DH5α |
1 |
E. coli K-12 is considered as having no or low individual and community risk. It is unlikely to cause human or animal disease. |
The organisms we used for this project :
Part number |
Source of the physical DNA |
What species does this part originally come from ? |
Risk Group |
The function of the parts |
BBa_K1472601 ('TesA) |
City University of Hong Kong |
Escherichia coli K 12 |
1 |
A protein that catalyzes the conversion of fatty acyl-ACP (acyl carrier protein) or fatty acyl-CoA to free fatty acids |
BBa_K1472606 (FadD & FadL) |
City University of Hong Kong |
Escherichia coli K 12 |
1 |
A TetR repressible construct encoding the FadD and FadL intermembrane proteins. |
BBa_K?????? |
Life Technologies Limited |
Synechocystis sp. PCC6803 |
1 |
A DNA construct containing △9 desaturase, △12 desaturase and △15 desaturase that work together to convert saturated fatty acid into ALA |
General public Safety :
All E. coli strains used in this project are attenuated mutant strains that are unlikely to survive/propagate in the environment outside the laboratory as they harbour many genetic mutations in their genome. To reduce the risk of biological materials escaping from the lab, all bacterial strains are kept and stored in designated refrigerators (before and after all experiments) in the supervisor’s (Dr. Richard Kong) laboratory which is not accessible to the general public. The lab where iGEM experiments are conducted is also not accessible by people of the general public because all lab workers need to be authorized to work in the lab and are required to carry a “Name badge”.