Team:Evry/Interlab Study/09-01-2014

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<h2>Construction n°1: PSB1C3 with I20260</h2>
 
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<h2>Other constructions of the Anderson library of constitutive promoters</h2>
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<br/><h2>Construction n°2: PSB1C3 with J23101-E1010</h2>
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PCR colony were performed on colonies from transformations of the 29th August. Used protocol was the same as Table 1 and 2 with the Q5 high fidelity enzyme.
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<br/> PCR product of first colony of each transformation was loaded on 1% agarose gel and gel run 45 minutes at 110 mV.
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    <center> PCR product of BBa_J61002 plasmids containing promoters results. </center>
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3 ml LB culture were started from tested colonies which presented the right PCR product profile. Cultures were incubated overnight at 37°C.
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<br/><h2> Construction n°3: PSB1C3 with K823012-E1010</h2>
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<h2>Constructions with other promoters</h2>
 
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<span class="cd-date">Sep 01</span>  
<span class="cd-date">Sep 01</span>  

Latest revision as of 02:07, 20 September 2014

Picture


Other constructions of the Anderson library of constitutive promoters


PCR colony were performed on colonies from transformations of the 29th August. Used protocol was the same as Table 1 and 2 with the Q5 high fidelity enzyme.
PCR product of first colony of each transformation was loaded on 1% agarose gel and gel run 45 minutes at 110 mV.
IMAGE
Symbol
PCR product of BBa_J61002 plasmids containing promoters results.
3 ml LB culture were started from tested colonies which presented the right PCR product profile. Cultures were incubated overnight at 37°C. Sep 01

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