Team:Evry/Interlab Study/08-28-2014

From 2014.igem.org

(Difference between revisions)
 
(5 intermediate revisions not shown)
Line 8: Line 8:
-
 
-
<h2>Contruction n°1: PSB1C3 with I20260</h2>
 
-
<br/>
 
<br/>
<br/>
Line 18: Line 15:
<br/>
<br/>
<br/><h2> Contruction n°3: PSB1C3 with K823012-E1010</h2>
<br/><h2> Contruction n°3: PSB1C3 with K823012-E1010</h2>
 +
<br/>PCR colony was done on 7 colonies:
 +
<ol>
 +
<li>Steril H2O:34,5µL
 +
<li>5X Q5 Reaction buffer: 10µL
 +
<li>Template:2µL
 +
<li>10mM dNTP: 1µL
 +
<li>10µM Primer forward VF2: 1µL
 +
<li>10µM Primer reverse VR: 1µL
 +
<li> Q5 DNA Polymerase: 0,5µL
 +
</ol>
 +
<br/>A migration of PCR product was done. We expect to observe in each wells a band of 1176bp:
 +
<br/><img src="https://static.igem.org/mediawiki/2014/b/b6/Evry2014_J23115-E0240_gel_migration_.jpg" alt="image not found" />
 +
<br/>
 +
<br/> 6 colonies have a band at 1200 bp around so we decided to choose colonies n°2, 3 and 4.
 +
<br/>
 +
<br/>
 +
<h2>Other constructions of the Anderson library of constitutive promoters</h2>
<br/>
<br/>
-
 
-
<h2>Constructions with other promoters</h2>
 
Promoters were resuspended from 2014 distribution kit plates with 10 µl of sterile MiliQ watter and stored at -20°C.
Promoters were resuspended from 2014 distribution kit plates with 10 µl of sterile MiliQ watter and stored at -20°C.
<div class="center">
<div class="center">

Latest revision as of 02:04, 20 September 2014

Picture



Contruction n°2: PSB1C3 with J23101-E1010




Contruction n°3: PSB1C3 with K823012-E1010


PCR colony was done on 7 colonies:
  1. Steril H2O:34,5µL
  2. 5X Q5 Reaction buffer: 10µL
  3. Template:2µL
  4. 10mM dNTP: 1µL
  5. 10µM Primer forward VF2: 1µL
  6. 10µM Primer reverse VR: 1µL
  7. Q5 DNA Polymerase: 0,5µL

A migration of PCR product was done. We expect to observe in each wells a band of 1176bp:
image not found

6 colonies have a band at 1200 bp around so we decided to choose colonies n°2, 3 and 4.

Other constructions of the Anderson library of constitutive promoters


Promoters were resuspended from 2014 distribution kit plates with 10 µl of sterile MiliQ watter and stored at -20°C.
IMAGE
Symbol
Table: Additional promoters of the Interlab Study. In grey, plasmids already resuspended, transformed, sequenced and stock glycerol existed.

Aug 28

Retrieved from "http://2014.igem.org/Team:Evry/Interlab_Study/08-28-2014"