Team:Evry/Interlab Study/08-23-2014

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<h2>Contruction n°1: PSB1C3 with I20260</h2>
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<br/>Digestion of PSB1C3 and PSB1K3-I20260 by EcoRI and PstI
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      <ul>
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        <li> 1 µl EcoRI-HF
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        <li> 1 µl PstI
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        <li> 2 µl buffer
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        <li> 0.2 µl BSA
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        <li> 13.7 or 76 µl respectively for I20260 and PSB1C3
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        <li> 10.8 µl miliQ watter
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      </ul>
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<br/>Incubation 45 minutes at 37°C. Denaturation step 20 minutes at 80 °C.
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<br/>Loading on a 1% agarose gel to perform an extraction from gel
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<br/><h2>Contruction n°2: PSB1C3 with J23101-E1010</h2>
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<span class="cd-date">Aug 23</span>  
<span class="cd-date">Aug 23</span>  

Latest revision as of 21:09, 19 September 2014

Picture

Contruction n°1: PSB1C3 with I20260


Digestion of PSB1C3 and PSB1K3-I20260 by EcoRI and PstI
  • 1 µl EcoRI-HF
  • 1 µl PstI
  • 2 µl buffer
  • 0.2 µl BSA
  • 13.7 or 76 µl respectively for I20260 and PSB1C3
  • 10.8 µl miliQ watter

Incubation 45 minutes at 37°C. Denaturation step 20 minutes at 80 °C.
Loading on a 1% agarose gel to perform an extraction from gel

Contruction n°2: PSB1C3 with J23101-E1010


Aug 23

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