Team:Evry/Interlab Study/08-23-2014

Contruction n°1: PSB1C3 with I20260

Digestion of PSB1C3 and PSB1K3-I20260 by EcoRI and PstI

• 1 µl EcoRI-HF
• 1 µl PstI
• 2 µl buffer
• 0.2 µl BSA
• 13.7 or 76 µl respectively for I20260 and PSB1C3
• 10.8 µl miliQ watter

Incubation 45 minutes at 37°C. Denaturation step 20 minutes at 80 °C.
Loading on a 1% agarose gel to perform an extraction from gel

Contruction n°2: PSB1C3 with J23101-E1010

Aug 23