Team:Caltech/week12
From 2014.igem.org
(Difference between revisions)
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<b>Export Systems</b> | <b>Export Systems</b> | ||
- | <ul> | + | <br>Redo of cloning of agr system: |
+ | <ul><li>Ran colony PCR on 10 picked overnight colonies.</li> | ||
+ | <li>colony PCR products were then run on a gel.</li> | ||
+ | <li>5 LB-CARB liquid cultures were set up and incubated overnight.</li> | ||
</ul> | </ul> | ||
- | < | + | De-FLAGging pTG005 for LC/MS analysis: |
- | < | + | <ul><li>Ran colony PCR on 10 picked overnight colonies.</li> |
- | </ | + | <li>colony PCR products were then run on a gel.</li> |
- | < | + | <li>3 LB-CARB liquid cultures were set up and incubated overnight.</li> |
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</ul> | </ul> | ||
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<b>Export Systems</b> | <b>Export Systems</b> | ||
- | <ul> | + | <br>Redo of cloning of agr system: |
- | </ul> | + | <ul><li>Miniprepped the 5 liquid cultures from yesterday. Stored the plasmids in the fridge.</li></ul> |
+ | De-FLAGging pTG005 for LC/MS analysis: | ||
+ | <ul><li>Miniprepped the 3 liquid cultures from yesterday. Stored the plasmids in the fridge.</li></ul> | ||
<b>lamBCDA & fsrABC Reception Systems</b> | <b>lamBCDA & fsrABC Reception Systems</b> | ||
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</ul> | </ul> | ||
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<b>lamBCDA & fsrABC Reception Systems</b> | <b>lamBCDA & fsrABC Reception Systems</b> | ||
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<b>Export Systems</b> | <b>Export Systems</b> | ||
- | <ul> | + | <ul><li>Shipped samples for both agr-recloning subproject (2*5=10 samples) & pTG005-unFLAGging subproject (2*3=6 samples) out for sequencing.</li> |
</ul> | </ul> | ||
<b>lamBCDA & fsrABC Reception Systems</b> | <b>lamBCDA & fsrABC Reception Systems</b> | ||
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<b>Export Systems</b> | <b>Export Systems</b> | ||
- | <ul> | + | <ul><li>Sequencing data appears to confirm successful unFLAGging of pTG005.</li> |
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</ul> | </ul> | ||
</td> | </td> |
Revision as of 01:00, 15 September 2014
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