Team:Caltech/week4
From 2014.igem.org
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<b><a href='week11'>Week 11</a></b><br><br> | <b><a href='week11'>Week 11</a></b><br><br> | ||
<b><a href='week12'>Week 12</a></b><br><br> | <b><a href='week12'>Week 12</a></b><br><br> | ||
+ | <b><a href='week13'>Week 13</a></b><br><br> | ||
+ | <b><a href='week14'>Week 14</a></b><br><br> | ||
+ | <b><a href='week15'>Week 15</a></b><br><br> | ||
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</td> | </td> | ||
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<b>Export Systems</b> | <b>Export Systems</b> | ||
<ul><li>PCR extracted vector backbone from pKS001 with Biobrick prefix & suffix overhangs</li> | <ul><li>PCR extracted vector backbone from pKS001 with Biobrick prefix & suffix overhangs</li> | ||
- | <li>PCR purification of PCR product and then 1 hour incubation at 37°C with DPN1 enzyme to remove any | + | <li>PCR purification of PCR product and then 1 hour incubation at 37°C with DPN1 enzyme to remove any unwanted fragments</li> |
<li>PCR purification of DPN1-digested product</li> | <li>PCR purification of DPN1-digested product</li> | ||
<li>Ran a gel that confirmed presence of ~3000bp-long DNA fragment in both DPN1-digested and undigested PCR products</li> | <li>Ran a gel that confirmed presence of ~3000bp-long DNA fragment in both DPN1-digested and undigested PCR products</li> | ||
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</ul> | </ul> | ||
<b>Combinatorial Promoters</b> | <b>Combinatorial Promoters</b> | ||
- | <ul> | + | <ul><li>Ran gel on Thursday's PCR products</li> |
+ | <li>DpnI digestion of PCR products to remove sticky ends</li> | ||
+ | <li>Gibson assembly of pAA003 plasmid</li> | ||
</ul> | </ul> | ||
</td> | </td> |
Latest revision as of 00:34, 15 September 2014
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