Team:Caltech/week2

From 2014.igem.org

(Difference between revisions)
 
(6 intermediate revisions not shown)
Line 3: Line 3:
<html>
<html>
-
 
+
<body style="background-color:#F6661F">
<!--main content -->
<!--main content -->
-
<table width="70%" align="center" border=1>
+
<table width="70%" align="center">
<!--welcome box -->
<!--welcome box -->
<tr>
<tr>
-
<td style="border:1px solid black;" colspan="3" align="center" height="150px" bgColor=#FF404B>
+
<td colspan="3" height="5px"> <center> <img src="https://static.igem.org/mediawiki/2014/0/0a/Caltech_logo.jpg" width="900" height="300">  
-
<h1 >WELCOME TO iGEM 2014! </h1>
+
<!-- If we ever want to put in a title banner, it goes here -->
-
<p>Your team has been approved and you are ready to start the iGEM season!
+
-
<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
+
-
<br>
+
-
<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Caltech/Notebook&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
+
</td>
</td>
</tr>
</tr>
Line 30: Line 26:
<tr heigth="75px">  
<tr heigth="75px">  
 +
<td align ="center"> <img src="https://static.igem.org/mediawiki/2014/4/42/Caltech_Plasy_%282%29.jpg" width="55px"></a> </td>
 +
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='black'; home.style.color='white'" onMouseOut="this.bgColor='white'; home.style.color='black'" bgColor=white> 
 +
<a id='home' href="https://2014.igem.org/Team:Caltech"style="color:#000000">Home </a> </td>
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='black'; team.style.color='white'" onMouseOut="this.bgColor='white'; team.style.color='black'" bgColor=white>
-
<a href="https://2014.igem.org/Team:Caltech"style="color:#000000">Home </a> </td>
+
<a id='team' href="https://2014.igem.org/Team:Caltech/Team"style="color:#000000"> Team </a> </td>
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
+
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='black'; profile.style.color='white'" onMouseOut="this.bgColor='white'; profile.style.color='black'" bgColor=white>
-
<a href="https://2014.igem.org/Team:Caltech/Team"style="color:#000000"> Team </a> </td>
+
<a id='profile' href="https://igem.org/Team.cgi?year=2014&team_name=Caltech"style="color:#000000"> Official Team Profile </a></td>
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
+
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='black'; project.style.color='white'" onMouseOut="this.bgColor='white'; project.style.color='black'" bgColor=white>
-
<a href="https://igem.org/Team.cgi?year=2014&team_name=Caltech"style="color:#000000"> Official Team Profile </a></td>
+
<a id='project' href="https://2014.igem.org/Team:Caltech/Project"style="color:#000000"> Project</a></td>
-
<td style="border:1px solid black" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='black'; parts.style.color='white'" onMouseOut="this.bgColor='white'; parts.style.color='black'" bgColor=white>
-
<a href="https://2014.igem.org/Team:Caltech/Project"style="color:#000000"> Project</a></td>
+
<a id="parts" href="https://2014.igem.org/Team:Caltech/Parts"style="color:#000000"> Parts</a></td>
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
+
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='black'; modeling.style.color='white'" onMouseOut="this.bgColor='white'; modeling.style.color='black'" bgColor=white>
-
<a href="https://2014.igem.org/Team:Caltech/Parts"style="color:#000000"> Parts</a></td>
+
<a id='modeling' href="https://2014.igem.org/Team:Caltech/Modeling"style="color:#000000"> Modeling</a></td>
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
+
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='black'; notebook.style.color='white'" onMouseOut="this.bgColor='white'; notebook.style.color='black'" bgColor=white>
-
<a href="https://2014.igem.org/Team:Caltech/Modeling"style="color:#000000"> Modeling</a></td>
+
<a id='notebook' href="https://2014.igem.org/Team:Caltech/Notebook"style="color:#000000"> Notebook</a></td>
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='black'; safety.style.color='white'" onMouseOut="this.bgColor='white'; safety.style.color='black'" bgColor=white><a id="safety" href="https://2014.igem.org/Team:Caltech/Safety"style=" color:#000000"> Safety </a></td>
-
<a href="https://2014.igem.org/Team:Caltech/Notebook"style="color:#000000"> Notebook</a></td>
+
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='black'; attributions.style.color='white'" onMouseOut="this.bgColor='white'; attributions.style.color='black'" bgColor=white>
-
<a href="https://2014.igem.org/Team:Caltech/Safety"style=" color:#000000"> Safety </a></td>
+
<a id="attributions" href="https://2014.igem.org/Team:Caltech/Attributions" style="color:black"> Attributions</a></td>
-
 
+
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
+
-
<a href="https://2014.igem.org/Team:Caltech/Attributions"style="color:#000000"> Attributions </a></td>
+
Line 62: Line 57:
</tr>
</tr>
</table>
</table>
-
 
+
<!-- End Navigation Menu -->
</tr>
</tr>
Line 69: Line 64:
</tr>
</tr>
   
   
-
 
-
 
Line 78: Line 71:
<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
<tr> <td colspan="3"  height="5px"> </td></tr>
<tr> <td colspan="3"  height="5px"> </td></tr>
 +
 +
<tr><td colspan=3>
<!--Actual Content Starts Here -->
<!--Actual Content Starts Here -->
-
<tr><td colspan="3"> <h3>Notebook</h3> Apparently there's a calendar feature to take care of this too???</td></tr>
+
<table>
-
</tr>
+
<tr> <td bgColor=#FFFFFF colspan = 3 height = 60px> <font size = +5> <center>Notebook </center> </td> </tr>
 +
 
<tr>
<tr>
-
<td>
+
<td style="padding: 20px" valign=top width=15%>
<!-- insert the links here -->
<!-- insert the links here -->
-
<br><br>
 
-
 
<b><a href='Notebook'>Overview</a></b><br><br>
<b><a href='Notebook'>Overview</a></b><br><br>
<b><a href='week1'>Week 1</a></b><br><br>
<b><a href='week1'>Week 1</a></b><br><br>
-
<b><a href='week2'>Week 2</a></b><br><br>
+
<b><font size=+1>Week 2</font></b><br><br>
-
<b><a href='week3'>Week 3</a></b><br><br>
+
<b><a href='week3'>Week 3</b><br><br>
<b><a href='week4'>Week 4</a></b><br><br>
<b><a href='week4'>Week 4</a></b><br><br>
<b><a href='week5'>Week 5</a></b><br><br>
<b><a href='week5'>Week 5</a></b><br><br>
Line 100: Line 94:
<b><a href='week10'>Week 10</a></b><br><br>
<b><a href='week10'>Week 10</a></b><br><br>
<b><a href='week11'>Week 11</a></b><br><br>
<b><a href='week11'>Week 11</a></b><br><br>
-
<b><a href='week12'>Week 12</a></b><br><br></td>
+
<b><a href='week12'>Week 12</a></b><br><br>
 +
<b><a href='week13'>Week 13</a></b><br><br>
 +
<b><a href='week14'>Week 14</a></b><br><br>
 +
<b><a href='week15'>Week 15</a></b><br><br>
 +
<!-- links end here -->
 +
</td>
-
<td valign=top>
+
<td valign=top style="padding: 20px">
-
<!-- insert the notebook entries for the week here -->
+
<!-- insert main content here -->
-
<table border=1>
+
<table>
-
<tr><td colspan=3><h1>Week Two</h1></td></tr>
+
<tr><td colspan=2><h1>Week Two</h1></td></tr>
<tr>
<tr>
Line 118: Line 117:
</ul>
</ul>
</td>
</td>
-
<td></td>
 
</tr>
</tr>
 +
 +
<tr><td bgColor="#777777" colspan="2" height="1px"></td></tr>
<tr>
<tr>
Line 132: Line 132:
</ul>
</ul>
</td>
</td>
-
<td></td>
 
</tr>
</tr>
 +
 +
<tr><td bgColor="#777777" colspan="2" height="1px"></td></tr>
<tr>
<tr>
Line 147: Line 148:
</ul>
</ul>
</td>
</td>
-
<td></td>
 
</tr>
</tr>
 +
 +
<tr><td bgColor="#777777" colspan="2" height="1px"></td></tr>
<tr>
<tr>
Line 158: Line 160:
     <li>Colony PCR and miniprep of bacterial colonies transformed with pAA002. Minipreps shipped for sequencing.</li>
     <li>Colony PCR and miniprep of bacterial colonies transformed with pAA002. Minipreps shipped for sequencing.</li>
</ul>
</ul>
 +
<hr>
 +
TXTL reaction data inconclusive. Most fluorescence signals are below negative control, and linear data frequently contradicts plasmid data with A90 promoter. No signal observed for B83 promoter.
</td>
</td>
-
<td>TXTL reaction data inconclusive. Most fluorescence signals are below negative control, and linear data frequently contradicts plasmid data with A90 promoter. No signal observed for B83 promoter.</td>
 
</tr>
</tr>
 +
 +
<tr><td bgColor="#777777" colspan="2" height="1px"></td></tr>
<tr>
<tr>
Line 172: Line 177:
</ul>
</ul>
</td>
</td>
-
<td></td>
 
</tr>
</tr>
 +
</table>
 +
<!-- End main content -->
</table>
</table>
</td>
</td>
-
 
+
</tr>
</table>
</table>
-
<br><br>
 
</html>
</html>

Latest revision as of 00:33, 15 September 2014
Home Team Official Team Profile Project Parts Modeling Notebook Safety Attributions
Notebook
Overview

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

Week Two

Monday, 6/23/14
  • Discussion of fallout from Friday's meeting, paper reading
  • Colony PCR and Minipreps of liquid cultures of colonies picked over the weekend
  • TXTL workshop starts today

Tuesday, 6/24/14
  • Discussed alternatives to current ComQXPA system, including 2-cell system (not all in 1 cell)
  • Gibson assembly of another combinatorial promoter using pKS001 backbone, lacI geneblock, B83 promoter geneblock, and sfGFP geneblock.
  • Transformation of Gibson constructs into JM109 cells
  • Linear combinatorial constructs created via PCR of pAA001 (minipreps) to be tested in TXTL

Wednesday, 6/25/14
  • Linear constructs and plasmid TXTL reactions of A90 promoter (pAA001) were set up and run
  • Gibson assembly of plasmid pAA002 containing B83 promoter
  • PCR of Gibson assemblies of pAA002 to create linear constructs to test in TXTL
  • TXTL reactions of B83 promoter (linear frag. from pAA002) were set up and run
  • Transformation of pAA002 Gibson assemblies into JM109

Thursday, 6/26/14
  • Analysis of yesterday's TXTL reactions (run overnight)
  • Colony PCR and miniprep of bacterial colonies transformed with pAA002. Minipreps shipped for sequencing.
TXTL reaction data inconclusive. Most fluorescence signals are below negative control, and linear data frequently contradicts plasmid data with A90 promoter. No signal observed for B83 promoter.

Friday, 6/27/14
  • Continued to investigate different signalling pathways
  • Potential alternative pathways to investigate:
    • fsrABCD
    • agrBDCA
    • uhpABCT

Retrieved from "http://2014.igem.org/Team:Caltech/week2"