Team:Caltech/week10

From 2014.igem.org

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<b>Export Systems</b>
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<br>Preparation of fsr system for LC/MS analysis:
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<ul><li>started vacufuging pAA001 acetonitrile samples (negative controls).</li>
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    <li>Colony PCR was run on 8 more colonies picked from pTG005-noFLAG plate (RTH reaction run last week).</li>
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    <li>Ran the PCR products on a gel.</li>
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    <li>Set up 6 LB-CARB liquid cultures corresponding to the promising colonies, as determined by the colPCR</li>
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<b>lamBCDA & fsrABC Reception Systems</b>
<b>lamBCDA & fsrABC Reception Systems</b>
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<ul><li>The 6 liquid cultures prepared yesterday were miniprepped and shipped for sequencing.</li>
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<b>lamBCDA & fsrABC Reception Systems</b>
<b>lamBCDA & fsrABC Reception Systems</b>

Revision as of 00:30, 15 September 2014
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Notebook
Overview

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week Ten

Monday, 8/18/14

 Export Systems
Preparation of fsr system for LC/MS analysis:
  • started vacufuging pAA001 acetonitrile samples (negative controls).
  • Colony PCR was run on 8 more colonies picked from pTG005-noFLAG plate (RTH reaction run last week).
  • Ran the PCR products on a gel.
  • Set up 6 LB-CARB liquid cultures corresponding to the promising colonies, as determined by the colPCR
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters
  • Performed another QuickLigation reaction using yesterday's round-the-horn PCR product.
  • Ligation reaction product was transformed into JM109.
  • Picked six colonies from yesterday's transformations (RTH) and restreaked on a new plate & started overnight liquid cultures.

Tuesday, 8/19/14

 Export Systems
  • The 6 liquid cultures prepared yesterday were miniprepped and shipped for sequencing.
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters
  • Performed colony PCR on the round-the-horn colonies obtained from Sunday's & Monday's transformations.
  • Sent promising colPCR products for sequencing.

Wednesday, 8/20/14

 Preparation of our powerpoint presentations for SURF seminar day (see Thursday's entry).

Thursday, 8/21/14

 Today is Summer Seminar Day (for SURF, our official summer research program)! All of us gave ~20-minute presentations on our individual subprojects.

Friday, 8/22/14

 Export Systems
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters

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