"
Team:TU Eindhoven/Click Reaction
From 2014.igem.org
Rafiqlubken (Talk | contribs) |
Rafiqlubken (Talk | contribs) |
||
| Line 33: | Line 33: | ||
</div> | </div> | ||
</div> | </div> | ||
| - | |||
<div class="wrap"> | <div class="wrap"> | ||
<div class="main"> | <div class="main"> | ||
| Line 39: | Line 38: | ||
<div class="clear"></div> | <div class="clear"></div> | ||
| - | + | <div class="cont-grid_left"> | |
<div class="Jasper"> | <div class="Jasper"> | ||
<span>Click Coli: expanding the chemical toolbox for bacteria</span> | <span>Click Coli: expanding the chemical toolbox for bacteria</span> | ||
| Line 46: | Line 45: | ||
</div> | </div> | ||
| - | + | <div class="clear"></div> | |
| - | </div> | + | </div> |
| - | </div> | + | |
| - | </div> | + | <div class="clear"></div> |
| - | + | </div></div> | |
| - | + | ||
</html> | </html> | ||
{{:Team:TU_Eindhoven/Template:Footer}} | {{:Team:TU_Eindhoven/Template:Footer}} | ||
Revision as of 05:55, 13 September 2014
Results: SPAAC on Cells
A fundamental problem in utilizing genetically modified bacteria is their limited ability to survive under non-natural conditions, such as the harsh conditions in industrial reactors and the immune system in the human body. We report a “Plug-and-Play” system allowing the introduction of chemical anchors on cell membranes, subsequently used to attach a functional coating. The anchors consist of azidophenylalanine, which couples covalently with molecules containing the strained alkyne DBCO in a so-called "click" reaction. We obtained proof of concept that our developed Clickable Outer Membrane Proteins (COMP) enable this fast and effective click reaction. We apply it to create a safe “clicked-on” coating allowing E.coli to be used in the human body for healthcare purposes. Furthermore, microfluidic devices have been designed, ensuring increased control over the click reaction and single cell coating. We believe our “Plug-and-Play” system is a versatile tool providing numerous possibilities for engineering bacteria on outer membranes.
