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Team:Bielefeld-CeBiTec/Notebook/Journal/C02-fixation/Jul
From 2014.igem.org
(Difference between revisions)
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<ul> | <ul> | ||
<li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Transformationviaelectroporation" target="_blank">Transformation</a> with electrocompotetent cells</li> | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Transformationviaelectroporation" target="_blank">Transformation</a> with electrocompotetent cells</li> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#ColonyPCR" target="_blank">Colony PCR</a> (<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#Primer1" target="_blank">Primer1</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#Primer2" target="_blank">Primer2</a>) | ||
| + | <ul> | ||
| + | <li>Annealing temperature: ...</li> | ||
| + | <li>Bands as expected (~2100 bp)</li> | ||
| + | <div class="element" style="width:150px"> | ||
| + | <a href="https://static.igem.org/mediawiki/2014/b/b1/Bielefeld_CeBiTec_2014-09-04_Gensynthese_Hneap_08_02.png" target="_blank"><img src="https://static.igem.org/mediawiki/2014/b/b1/Bielefeld_CeBiTec_2014-09-04_Gensynthese_Hneap_08_02.png" width="150px"></a><br><font size="1">Agarose gel from colony PCR. As a Ladder we used <a href="http://www.thermoscientificbio.com/nucleic-acid-electrophoresis/generuler-1-kb-dna-ladder-ready-to-use-250-to-10000-bp">GeneRuler™ 1 kb DNA Ladder from Thermo Scientific</a>. </font> | ||
| + | </div> | ||
| + | <li>We plated sample number 1 for a plasmid isolation.</li> | ||
| + | </ul> | ||
| + | |||
| + | </li> | ||
| + | |||
| + | |||
<li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of Hneap</li> | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of Hneap</li> | ||
</ul> | </ul> | ||
Revision as of 18:00, 4 September 2014
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July |
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- pSB1C3 backbone
- This week we tried to amplify parts for the Gibson Assembly
- PCR amplification (Primer1, Primer2)
- Annealing temperature: ...
- Bands as expected (~2070 bp)
- Purification of the backbones with gel extraction
- pHnCBcsoS1D
- This week we aimed to isolate the plasmid of the strain ordered from addgene
- Plasmid isolation of pHnCBcsoS1D
- csoS1D of the carboxysome
- This week we tried to amplify different parts of the carboxysome.
- PCR amplification (Primer1, Primer2)
- Annealing temperature: ...
- Bands as expected (~703 bp)
- Carbonic anhydrase (can) of the carboxysome
- This week we tried to amplify different parts of the carboxysome.
- PCR amplification (Primer1, Primer2)
- Annealing temperature: ...
- Bands (not) as expected (... bp)
- Shell associated protein of the carboxysome (first protein)
- This week we tried to amplify different parts of the carboxysome.
- PCR amplification (Primer1, Primer2)
- Annealing temperature: ...
- Bands as expected (... bp)
- csoS1D
- This week we tried to transform the construct.
- Gibson Assembly with csoS1D on pSB1C3
- Transformation with electrocompotetent cells
- Colony PCR (Primer1, Primer2)
- Annealing temperature: ...
- Bands not as expected (... bp). Size looked like template insert CFP.
- Restriction digestion with DpnI
- Bands (not) as expected (... bp)
- Carbonic anhydrase (can)
- This week we tried to transform the construct.
- Gibson Assembly with can and pSB1C3
- Transformation with electrocompotetent cells
- Transformation was not succesful.
- pHnCBcsoS1D backbone
- This week we tried to amplify the backbone of the plasmid.
- PCR amplification (Primer1, Primer2)
- Annealing temperature: ...
- Bands as expected (~13,200 bp)
- BioBricks (BBa_k731500 and BBa_Q01400)
- This week we tried to use promotors of two BioBricks of the parts distribution.
- Plasmid isolation of pSB1C3_pTac and pSB1C3_p_TetR
- csoS4AB
- This week we tried amplifiy shell proteins of the carboxysome.
- PCR amplification (Primer1, Primer2)
- Annealing temperature: ...
- Bands as expected (... bp)
- csoS1CAB
- This week we tried amplifiy shell proteins of the carboxysome.
- PCR amplification (Primer1, Primer2)
- Annealing temperature: ...
- Bands as expected (... bp)
- Carbonic anhydrase
- This week we tried to purify the construct from the gel.
- Purification of can with gel extraction
- Bands not as expected (... bp)
- Shell associated protein
- This week we tried to purify the construct from the gel.
- Purification of can with gel extraction
- Bands as expected (~1235 bp)
- Pore protein (csoS1D) of the carboxysom
- This week we tried to purify the construct from the gel.
- Purification of can with gel extraction
- Bands not as expected (~703 bp)
- SBPase (glpX)
- This week we tried to amplify both parts of glpX.
- PCR amplification (Primer1, Primer2 and Primer3, Primer4)
- Annealing temperature: ...
- Bands (not) as expected (... bp)
- Carbnonic anhydrase (can)
- This week we tried to transform the construct.
- Gibson Assembly with csoS1D and pSB1C3
- Restriction digestion with DpnI
- Bands (not) as expected (... bp)
- Colony PCR (Primer1, Primer2)
- Annealing temperature: ...
- Bands as expected (1900 bp)
- Plasmid isolation of can
- csoS1D
- This week we tried to transform the construct.
- Gibson Assembly with csoS1D and pSB1C3
- Restriction digestion with DpnI
- Bands (not) as expected (... bp)
- Colony PCR (Primer1, Primer2)
- Annealing temperature: ...
- Bands as expected (1000 bp)
- Restriction digestion with EcoRI and XbaI
- Bands (not) as expected (... bp)
- Plasmid isolation of csoS1D
- Phosphoribulokinase (prkA)
- This week we tried to transform the fragments of the gene synthesis
- Transformation with electrocompotetent cells
- Plasmid isolation of prkA
- sRNA:pfkA
- This week we tried to transform the fragments of the gene synthesis
- Transformation with electrocompotetent cells
- Plasmid isolation of sRNA:pfkA
- RuBisCO of H. neapolitanus
- This week we tried to transform the fragments of the gene synthesis
- Transformation with electrocompotetent cells
- Colony PCR (Primer1, Primer2)
- Annealing temperature: ...
- Bands as expected (~2100 bp)
- We plated sample number 1 for a plasmid isolation.
- Plasmid isolation of Hneap
- RuBisCO of S. elongatus
- This week we tried to transform the fragments of the gene synthesis
- Transformation with electrocompotetent cells
- BioBricks (BBa_I719005)
- This week we tried to use the promotor of the BioBrick of the parts distribution.
- Plasmid isolation of pSB1C3_T7 BBa_I719005
