Team:Groningen/Template/MODULE/project/MBD

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 </html>{{:Team:Groningen/Template/MODULE/tmpl_textmodule|Why modeling?|''Staphylococcus aureus'' and ''Pseudomonas aeruginosa'' are the two pathogens that cause infection in the burn wounds. The aim of our project was to design a smart bandage that can produce Infection Prevention Molecules (IPMs) only in the presence of these two pathogens. The bandage has freeze-dried ''Lactococcus lactis'' cells which are activated by hydrating the gel. The hydrated gel contains all the nutrient supplements that are required for the ''L. lactis'' to grow. The quorum molecules produced by the pathogens in the wound diffuse through the hydrogel and reach the ''L. lactis'' . This triggers the production of IPMs. The IPMs which are produced by the genetically engineered ''L. lactis'' in the hydrogel must diffuse to the site of infection in the burn wounds. Understanding the diffusion of IPMs is important for us to design a smart bandage which is quite realistic. Based on the bandage requirements there were different possible designs. Modeling those designs helped us to choose the best design and study the diffusion characteristics in our bandage. The best design was further analyzed to obtain information regarding the life time of the bandage after it is being activated. The bandage model gives an overall idea about production rate of IPMs, diffusion of the IPMs, inimum number of bacteria that should be available in the bandage and nutrient consumption. The above model shows the macroscopic details of the bandage. To study the genetic circuit that plays a vital role in production of IPMs in the presence of the pathogens, a cellular level model was developed. The genetically engineered ''L. lactis'' produces nisin and DspB in response to the Auto-Inducing Peptides produced by ''S. aureus''. Aiia and DspB are produced only in the presence of AHLs produced by the ''P. aeruginosa''. 

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