Template:JS/EPFL timeline json

From 2014.igem.org

{"timeline":{"headline":"Follow us throughout the summer","type":"default","text":" ","date":[{"startDate":"2014,10,18","endDate":"2014,10,19","headline":"Our last post!! 5:30 am, 30 minutes before Wikifreeze","text":"

Final data:<\/p>Facebook Likes: 250<\/p>Coffee consumption: 600L<\/p>PCRs: 789<\/p>Average sleep during Wikifreeze week: 4:30 h\/night<\/p>Last update: 5 min before Wikifreeze<\/p>","tag":"General","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/2\/2d\/LastPost_.jpg","caption":""}},{"startDate":"2014,10,17","endDate":"2014,10,18","headline":" Thank you to our friends who support us!","text":"
<\/p>","tag":"General","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/e\/e5\/Timeline_petit_dej.png","caption":""}},{"startDate":"2014,10,12","endDate":"2014,10,13","headline":"Pbs2 tagged with Renilla Luciferase characterisation","text":"The fusion protein Pbs2-rLuciferase is correctly expressed in our cells ! <\/p>","tag":"Yeast","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/0\/0e\/Pbslucprettycomparaison_.png","caption":""}},{"startDate":"2014,10,15","endDate":"2014,10,16","headline":"SplitGFP before vs after acetic acid stress","text":"
<\/p>","tag":"Yeast","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/3\/3b\/Splitgfpyeast.jpg","caption":""}},{"startDate":"2014,09,20","endDate":"2014,09,21","headline":"Analysis of the spatial dynamics of CpxR-Split IFP activation by microscopy","text":"We could localize the IFP signal within a cell responding to stress","tag":"Bacteria","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/0\/08\/EPFL_2014_03_10_2014_Experiment-37.jpg","caption":""}},{"startDate":"2014,09,16","endDate":"2014,09,17","headline":"Split-IFP: activation and deactivation of the signal by changing the medium","text":"We could manage to shut down the signal by removing the PBS-KCL and replacing it by neutral PBS<\/p>","tag":"Bacteria","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/6\/61\/KCL_titration_green_small_EPFL.jpg","caption":""}},{"startDate":"2014,08,11","endDate":"2014,08,12","headline":"Last of four combinations of Split-CpxR achieved!","text":"IFP1 and IFP2 at the C terminal of CpxR. Here you can see the restriction site analysis of the construct. All the colonies we picked after the Gibson Assembly and Transformation contained the expected sequence, except the 11th. <\/p>","tag":"Bacteria","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/7\/7e\/GEL3.png","caption":""}},{"startDate":"2014,07,27","endDate":"2014,07,28","headline":"Fusion of CpxR-IFP2 and CpxR-IFP1 in the same plasmid","text":"Bacteria will have to synthesize both IFP1-CpxR IFP2-CpxR. <\/span>4 combinations, 4 more Gibson Assembly...<\/p>","tag":"Bacteria","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/3\/3b\/GA1-4.png","caption":""}},{"startDate":"2014,07,19","endDate":"2014,07,20","headline":"Gibson assembly to fuse IFP2 and CpxR, at its C or N terminal","text":"To insert IFP2 at the C or N terminal of CpxR, the vector and the insert have been amplified by PCR with suitable primers to create overlaps and make a Gibson assembly. We obtained our two new constructs!<\/p>","tag":"Bacteria","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/a\/ae\/IFP2.png","caption":""}},{"startDate":"2014,07,25","endDate":"2014,07,26","headline":"Gibson assembly to fuse IFP1 and CpxR, at its C or N terminal","text":"The restriction site analysis revealed that we obtained the expected fragments for all the colonies. Check out our labbook!<\/p>","tag":"Bacteria","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/2\/26\/Capture_d%E2%80%99%C3%A9cran_2014-10-15_%C3%A0_12.11.12.png","caption":""}},{"startDate":"2014,07,16","endDate":"2014,07,17","headline":"We're starting to work on the Split IFP !","text":"We obtained the plasmids from Michnick lab, containing IFP[1] and IFP[2] sequences. We could transform competent cells with these plasmids, grow them, recover high amount of plasmids and isolate the sequences of interest by PCR.<\/p>","tag":"Bacteria","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/2\/2c\/Ifp.png","caption":""}},{"startDate":"2014,07,17","endDate":"2014,07,18","headline":"First BioBrick!","text":"We successfully achieved our first BB, pCpxR<\/span>, a plasmid containing the CpxR coding sequence under the arabinose responsive promoter in the pSB1C3 backbone. Here you can see the result of the colony PCR, of expected size of 2254 bp.<\/p>","tag":"Bacteria","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/c\/c5\/Week2.png","caption":""}},{"startDate":"2014,10,13","endDate":"2014,10,14","headline":"Our savior !","text":"Niko rode his bike to buy huge pizzas for the team so we could eat something during one of our wiki night sessions!<\/p>","tag":"General","tag_list":"wiki","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/5\/55\/FullSizeRender.jpg","caption":""}},{"startDate":"2014,10,12","endDate":"2014,10,13","headline":"Wik End","text":"
<\/p>","tag":"General","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/d\/da\/1910004_10205017863314839_2748819972307868872_n.jpg","caption":""}},{"startDate":"2014,08,04","endDate":"2014,08,05","headline":"Chip factory day","text":"Made a stock of chips today<\/p>","tag":"Microfluidics","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/6\/62\/Greg8.JPG","caption":""}},{"startDate":"2014,08,30","endDate":"2014,08,31","headline":"No more delamination!","text":"As we used our SmashColi chip, we wanted to apply high pressure but delimitation would occur.<\/p>To avoid this we plasma treated the chip and the glass.<\/p>","tag":"Microfluidics","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/9\/93\/Greg12.JPG","caption":""}},{"startDate":"2014,09,12","endDate":"2014,09,13","headline":"Sakura correcting highschool student tests","text":"
<\/p>","tag":"General","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/a\/a1\/Timeline3.JPG","caption":""}},{"startDate":"2014,10,11","endDate":"2014,10,12","headline":"saturday evening meeting ","text":"
<\/p>","tag":"General","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/1\/15\/Timeline2.JPG","caption":""}},{"startDate":"2014,10,08","endDate":"2014,10,09","headline":"We got our split Renilla Luciferase and Split GFP yeast strains !","text":"The cotransformations worked out and we can pick colonies on the plates to start the experiements.<\/p>","tag":"Yeast","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/a\/a8\/IMG_0622.JPG","caption":""}},{"startDate":"2014,10,10","endDate":"2014,10,11","headline":"Working on the wiki!","text":"
<\/p>","tag":"General","tag_list":"team,wiki","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/2\/21\/Wikidoc.jpg","caption":"Arthur, Ione, and Sakura"}},{"startDate":"2014,09,30","endDate":"2014,10,01","headline":"CpxR--Split-IFP in the chip","text":"It works! (maybe...)<\/p>","tag":"Microfluidics","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/6\/64\/Photo_30.09.14_11_09_05.jpg","caption":""}},{"startDate":"2014,10,10","endDate":"2014,10,11","headline":"Peanut incident on our assistant's desk...","text":"Come on, it's not that messy!<\/p>","tag":"Microfluidics","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/4\/47\/P1080924.JPG","caption":""}},{"startDate":"2014,09,28","endDate":"2014,09,29","headline":"Documentation day.","text":"We spent the whole day working on the documentation and on the wiki.
<\/p>","tag":"General","tag_list":"team","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/b\/b2\/IGEM_EPFL_docsprint_hackuarium20140928.jpg","caption":""}},{"startDate":"2014,10,06","endDate":"2014,10,07","headline":"Sport session!","text":"
<\/p>","tag":"General","tag_list":"team,running","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/f\/f5\/Team_running.jpg","caption":""}},{"startDate":"2014,09,26","endDate":"2014,09,27","headline":"Elephant made by the high school students","text":"The plating of cells expressing chromoproteins had awesome results!
<\/p>","tag":"General","tag_list":"team","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/2\/2a\/Hp_elephant.JPG","caption":""}},{"startDate":"2014,07,09","endDate":"2014,07,10","headline":"CpxR PCR amplification ","text":"After genome extraction of E. Coli strain K-12 MG1655, CPXR was amplified by Phusion PCR <\/p>","tag":"Bacteria","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/b\/bf\/EFPL_IFP_Gel1.png","caption":""}},{"startDate":"2014,09,24","endDate":"2014,09,25","headline":"Visit of 80 highschool students","text":"80 highschool students came at EPFL to visit us and hear about our project. They streaked bacteria, loaded agarose gels and played the mini iGEM game. We had a lot of fun!","tag":"General","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/0\/07\/IMG_3457.JPG","caption":""}},{"startDate":"2014,09,27","endDate":"2014,09,28","headline":"Our project hit the headlines!","text":"An article about our BioPad was published in \"Le Temps\". We had great comments from Rolf Heuer (Director of the CERN) and Bent Stumpe (the inventor of the touchscreen himself!).<\/p>","tag":"General","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/8\/8d\/20140927_01_%282%29.jpg","caption":""}},{"startDate":"2014,09,19","endDate":"2014,09,20","headline":"Our favorite protocol","text":"After a hard day in the lab we like to take our bikes and climb up Lausanne before eating the best burrito in town.<\/p>","tag":"General","tag_list":"team,bike","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/0\/02\/Bike.JPG","caption":"Axel, Niko, Thomas and Greg"}},{"startDate":"2014,09,08","endDate":"2014,09,09","headline":"Reconfirmation of CpxR Split IFP experiment","text":"We relaunched the experiment and obtained the same results as the previous experiment ! This sounds promising !<\/p>","tag":"Bacteria","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/5\/56\/GA1_means_10_09_14.png","caption":""}},{"startDate":"2014,09,05","endDate":"2014,09,06","headline":"Discovery of CpxR dimerization in E.Coli and that it interacts with itself via its C terminal by split IFP PCA","text":"At 2:00 in the morning we finally got the expected result: fast dimerization and signal emission upon stress !<\/p>","tag":"Bacteria","tag_list":"IFP,CpxR,Split,PCA,IFP1.4","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/4\/4d\/IFP_Experiment_07_09_14.png","caption":""}},{"startDate":"2014,09,12","endDate":"2014,09,13","headline":"One of our genes of interest is sfGFP tagged ","text":"First fluorescent yeast cells ! We've been waiting for it for quite a long time ... !<\/p>","tag":"Yeast","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/c\/c3\/Photo_2.JPG","caption":""}},{"startDate":"2014,09,11","endDate":"2014,09,12","headline":"First Transformed Yeasts Cultures","text":"Four transformations worked well and we can now start some experiments with our new strains !","tag":"Yeast","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/e\/e4\/Photo_1_%281%29.JPG","caption":""}},{"startDate":"2014,08,04","endDate":"2014,08,05","headline":"International iGEMers feet picture contest","text":"I let you admire the beauty of these feet... No matter the surroundings, they look perfect... Yes, we do have fun sometimes !<\/p>","tag":"General","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/e\/e6\/Feet.JPG","caption":""}},{"startDate":"2014,09,01","endDate":"2014,09,02","headline":"We definitely love them ","text":"Rainbow bacteria!<\/p>","tag":"General","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/0\/05\/Love.JPG","caption":""}},{"startDate":"2014,08,14","endDate":"2014,08,15","headline":"Playing with chromoproteins !","text":"We just receive the bacteria expressing super colorful chromoproteins, we'll obviously try to draw some stuff with it later !<\/p>","tag":"General","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/1\/1a\/Chromo.jpg","caption":""}},{"startDate":"2014,07,07","endDate":"2014,07,08","headline":"We finally ordered the plasmids","text":"The plasmids with the appropriate yeast selection markers were finally successfully ordered after several days of doubts!<\/p>","tag":"Yeast","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/c\/c8\/PSfGFP-Ura3_Map.png","caption":""}},{"startDate":"2014,07,21","endDate":"2014,07,22","headline":"First Meeting with Prof. Simanis and Andrea Krapp","text":"We could finally get an answer to most of our questions regarding our final constructs. They helped us a lot with the different protocols used to work with yeasts and they confirmed that we had chosen the right strategy to implement the split luciferase system in this organism! Thank you !<\/p>","tag":"Yeast","tag_list":"","asset":{"media":"","caption":""}},{"startDate":"2014,07,08","endDate":"2014,07,09","headline":"Team photo!","text":"Bastien, Lucie, Ione, Sakura, Niko, I\u00e7vara, Axel, Thomas, Arthur, Jin, Greg, Romane, C\u00e9cile, Ted

Check out our team page to know more!
<\/p>","tag":"General","tag_list":"team","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/2\/2c\/Team_pic_sitting.jpg","caption":"Students of this year's team"}},{"startDate":"2014,09,01","endDate":"2014,09,02","headline":"Our IT guy in the lab!","text":"We actually got him to put on some gloves ;)","tag":"I.T","tag_list":"plates","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/f\/fc\/10628156_810860178945761_7218458097167179669_n.jpg","caption":"Arthur in the lab!"}},{"startDate":"2014,08,07","endDate":"2014,08,08","headline":"Characterization of the arabinose promoter","text":"Increasing GFP intensity due to increasing arabinose concentrations from right to left.<\/p>","tag":"Bacteria","tag_list":"CpxR-GFP,Arabinose","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/0\/07\/10506849_805516152813497_937295338237678710_o.jpg","caption":"Increasing GFP intensity from right to left"}},{"startDate":"2014,07,19","endDate":"2014,07,20","headline":"Luciferases in the MITOMI","text":"Renilla luciferase expression experiment that didn't go well... But still nice picture!
<\/p>","tag":"Microfluidics","tag_list":"luciferase","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/d\/df\/Pynzc.PNG","caption":""}},{"startDate":"2014,07,15","endDate":"2014,07,16","headline":"GFP and Superfolded GFP","text":"This is a scan of the MITOMI chip containing two types of reporters: normal GFP and superfolded GFP which is more intense
<\/p>","tag":"Microfluidics","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/f\/fe\/Gfp.png","caption":""}},{"startDate":"2014,07,21","endDate":"2014,07,22","headline":"General meeting on monday morning","text":"Weekly meetings on monday morning at the lab... So serious !<\/p>","tag":"General","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/b\/bb\/Photo_%284%29.JPG","caption":"EPFL Team "}},{"startDate":"2014,07,30","endDate":"2014,07,31","headline":"Late night labwork","text":"Late night agar plates session with Axel, Thomas and Greg<\/p>","tag":"Bacteria","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/c\/c0\/Photo_%285%29.JPG","caption":"From left to right: Axel, Thomas, Greg"}},{"startDate":"2014,07,14","endDate":"2014,07,15","headline":"Making our MITOMI chip","text":"This is the design of a MITOMI chip.
<\/p>","tag":"Microfluidics","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/b\/b1\/Mitomi.PNG","caption":"Adapted from Rockel, S., Geertz, M., & Maerkl, S. J. (2012). MITOMI: A Microfluidic Platform for In Vitro Characterization of Transcription Factor\u2013DNA Interaction. In Gene Regulatory Networks (pp. 97-114). Humana Press."}},{"startDate":"2014,07,14","endDate":"2014,07,15","headline":"First GFP expressing bacteria!","text":"This is a pellet of our first GFP (Green Fluorescent Protein) bacteria.<\/p>","tag":"Bacteria","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/c\/c3\/Photo_%283%29.JPG","caption":"GFP expressing bacteria"}},{"startDate":"2014,08,21","endDate":"2014,08,22","headline":"Bioluminescence assay","text":"This is a bioluminescence assay of Firefly and Renilla luciferases and their respective splits. <\/p>","tag":"Bacteria","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/e\/e6\/Bioluminescence_21.08.14.PNG","caption":""}},{"startDate":"2014,08,28","endDate":"2014,08,29","headline":"Voice over","text":"We recorded the voice over for our presentation video<\/p>","tag":"I.T","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/7\/73\/10660265_809193322445780_320976615889235034_n.jpg","caption":""}},{"startDate":"2014,07,17","endDate":"2014,07,18","headline":"First day of clean room training!","text":"
<\/p>","tag":"Microfluidics","tag_list":"","asset":{"media":"http:\/\/2014.igem.org\/wiki\/images\/thumb\/2\/2f\/Microfluidics.jpg\/450px-Microfluidics.jpg","caption":""}}]}}