Team:UT-Dallas/Notebook/7-21

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Saturday, July 21st, 2014

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Overnight Ligation for tcpB, tcpD, and tcpQ with a negative control

Test Digestion and Gel Electrophoresis for i5.19.1 and i5.19.2

annealing

Gel Purification and Extraction for tcpT, acfA, acfB, acfC, and acfD


gel for i5.19.1 and 15.19.2 we kept 15.19.1 because the band proved we had the digested DNA however for 15.19.2 there was still dome digested DNA so we discarded it


Gel purification for tcpT, acfA, acfB, acfC, and acfD using a 50bp ladder.

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