07/28/2014

Today we are going to test our enzymes with their buffers to build our biobricks! We’ll digest E0840 with EcoRI, SpeI, XbaI, PstI using the following digestion system:

Final volume of 10ul and digestion at 37°C for 1 hora.

The result was great!!!! EcoRI Works very well with either Buffer 1 or Buffer 2 from New England Biolabs. SpeI e XbaI had a great performance with Buffer 2 and PstI great performance with Buffer 3!!!! =)