Team:Sheffield/Prot2

From 2014.igem.org

Protocol 2 - Produce LB Agar

Materials and Equipment

LB Agar mix, sterile water, weighing scales, weighing boat, conical flask, measuring cylinder.

Time

Prep: 5 minutes
Run: 2 hours

Procedure

Weigh out 3.5g of LB agar mix in a weighing boat and add into a conical flask
Measure 100ml sterile water into a measuring cylinder and dispense into the same conical flask.
Swirl.
Stopper the flask with cotton wool and foil.
Label on autoclave tape. Format 'iGEM, Room Number'.
Move the flask(s) to the autoclave to be sterilised.