Team:Sheffield/Prot2
From 2014.igem.org
Protocol 2 - Produce LB Agar
Materials and Equipment
LB Agar mix, sterile water, weighing scales, weighing boat, conical flask, measuring cylinder.Time
Prep: 5 minutesRun: 2 hours
Procedure
Weigh out 3.5g of LB agar mix in a weighing boat and add into a conical flaskMeasure 100ml sterile water into a measuring cylinder and dispense into the same conical flask.
Swirl.
Stopper the flask with cotton wool and foil.
Label on autoclave tape. Format 'iGEM, Room Number'.
Move the flask(s) to the autoclave to be sterilised.