"
Team:SCUT-China/Safety
From 2014.igem.org
Safety
The chassis organisms we used for this project
| Species name (including strain) | Risk Group | Disease risk to humans? |
| E. coli(K12) BL21 | 1 | Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. However, E.coli is not considered a human or animal pathogen nor is it toxicogenic. |
| E. coli(K12) TOP10 | 1 | Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. However, E.coli is not considered a human or animal pathogen nor is it toxicogenic. |
| E. coli(K12) DH5alpha | 1 | Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. However, E.coli is not considered a human or animal pathogen nor is it toxicogenic. |
| Saccharopolyspora erythraea | 1 | No |
| Bacillus subtilis 168 | 1 | No |
List and description of all new or modified coding regions we used
| Part number/name | What species does this part originally come from? | Risk Group | Natural function of part | How will you use it? |
| BBa_K1371000 (PKS) | Saccharopolyspora erythraea | 1 | Produce the macrolide antibiotic erythromycin | This part produces the macrolide antibiotic which our promoter is designed to detect |
| BBa_K1371011 (sfp) | Bacillus subtilis 168 | 1 | Code for PPTase | This part is code for PPTase which can activate ATP |
| BBa_K1371012 (birA) | E. coli(K12) BL21 | 1 | Code for biotin ligase | Enhance the activity of pccB upon coexpression |
| BBa_K1371013 (pccB) | Streptomyces coelicolor | 1 | carboxyl transferase, β subunit of pcc | This part produces the propionyl-CoA with combination of accA |
| BBa_K1371014 (accA) | Streptomyces coelicolor | 1 | acetyl-CoA carboxylase, biotinylated α subunit of pcc | This part produces the propionyl-CoA with combination of pccB |
| BBa_K1371016 (docking domain1) | Saccharopolyspora erythraea | 1 | Code for docking domain which is required efficient transfer of polyketide intermediates between polypeptides | efficient transfer of polyketide intermediates between polypeptides |
| BBa_K1371017 (docking domain2) | Saccharopolyspora erythraea | 1 | Code for docking domain which is required efficient transfer of polyketide intermediates between polypeptides | efficient transfer of polyketide intermediates between polypeptides |
| BBa_K1371018 (docking domain3) | Saccharopolyspora erythraea | 1 | Code for docking domain which is required efficient transfer of polyketide intermediates between polypeptides | efficient transfer of polyketide intermediates between polypeptides |
| BBa_K1371019 (docking domain4) | Saccharopolyspora erythraea | 1 | Code for docking domain which is required efficient transfer of polyketide intermediates between polypeptides | efficient transfer of polyketide intermediates between polypeptides |
| BBa_K1371020 (docking domain5) | Saccharopolyspora erythraea | 1 | Code for docking domain which is required efficient transfer of polyketide intermediates between polypeptides | efficient transfer of polyketide intermediates between polypeptides |
| BBa_K1371021 (docking domain6) | Saccharopolyspora erythraea | 1 | Code for docking domain which is required efficient transfer of polyketide intermediates between polypeptides | efficient transfer of polyketide intermediates between polypeptides |
Do the biological materials used in our lab work pose any of the following risks? Every species we worked with belongs to BL1. They do no harm to human. Experimenters is careful not to release any parts to the public. And even the worst situation happens as it is released, it will hardly bring risks.
a)
Risks to the safety and health of team members, or other people working in the lab:
Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. However, E.coli is not considered a human or animal pathogen nor is it toxicogenic. Any concerns for E.coli in terms of health considerations are mitigated by its poor ability to colonize the colon and establish infections.
b)
Risks to the environment (from waste disposal, or from materials escaping from your lab):
The plasmids we designed cannot function outside certain cell lines in the laboratory, And the cell we used is very fragile and it will soon die leaving the culture medium. So it will not bring contamination to the environment.
c)
Risks to security through malicious mis-use by individuals, groups, or countries:
All the biological materials used in our lab are safe, and all the products of the coding sequences are innocuous to human, so the risk of malicious misuse is low.
A regular safety briefing and a lecture about the basics concerning of biotechnology and genetic engineering are basic elements of our education at the SCUT.
The handling of biological material and safety aspects of chemicals are explained.
A safety briefing was held for all iGEM students by Zhenwu Zhang who is the responsible person for our lab safety.
