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From 2014.igem.org

What’s the kind of particle you want to sendiment?
Protein
DNA
Cell/bacteria
What’s its molecular/particle weight(g/mol)？
what’s the density of your buffer(g/ml)？(0 if you have no idea about it)
What’s the diffusion coeeficient of your buffer(cm²/sec)? (0 if you have no idea about it)
What’s the temperature in your rotor(℃)?

Your rotor has to be a least at the speed of to sendiment the particle