Team:IIT Delhi/Notebook
From 2014.igem.org
- ✔ Week 1,2,3,4: Our initial week's work comprised of sessions in which our team had lab safety training and learned how to implement different protocols. Ex: Genomic DNA Extraction, Gel Electrophoresis etc. Click here to see the notebook of these weeks
- ✔ Week 5-6-7-8: These week's work consists of more protocol implementation. Ex: Restriction Digestion, Ligation, 3A(RFC10) Assembly etc. and it also includes brainstorming the problem solving Click here to see the notebook of these weeks
- ✔ Week 9-10 : Here we started performing actual experiments involved in the project Click here to see the notebook of these weeks
- ✔ Week 11 : Plasmid Preps, Gel Runs , Restriction digestions and Ligations as our 3A assembly failed initially. Click here to see the notebook of this week
- ✔ Week 12 : More and more Preps, Runs , Digestions and Ligations. Click here to see the notebook of this week
- ✔ Week 13 : More and more Preps, Runs , Digestions and Ligations. Click here to see the notebook of this week
- ✔ Week 14 : And finally we hit on the target, our first clone , nrfA it is Click here to see the notebook of this week
- ✔ Week 16-17-18-19 : Now its time for other clones, More and more preps, runs , digestions, ligations and PCRs Click here to see the notebook of these weeks
- ✔ Week 23-24-25 : And finally we got our clones !!! Click here to see the notebook of these weeks