See below all the Materials we used. For the individual experiments visit our Notebook, the MidnightDoc, for a collection of our Methods please see Methods.
Name | Sequence
|
VF2 | tgccacctgacgtctaagaa
|
VR | attaccgcctttgagtgagc
|
pSB1C3_F_SspDnaE-N-taataat | GACGCAGGTACAATTAAATAATAATACTAGTAGCGGCCGCTGCAG
|
pSB1C3_R_Flag-SspDnaE_C | CTTTAACCTTGTCATCATCGTCTTTGTAGTCCATCTAGTACTTTCCTGTGTGACTCTAGAAGCG
|
SspDnaE-C_F_pSB1C3-Flag | TACTAGATGGACTACAAAGACGATGATGACAAGGTTAAAGTTATCGGTCGTCGATCCC
|
SspDnaE-C_R_BsaI-ccdB | CTCCCTTATACACAGCCAGTGGTCTCGAATTGTTTGTTAAAACAGTTGGCGGCGATAG
|
ccdB_F_SspDnaE-C-BsaI | TTAACAAACAATTCGAGACCACTGGCTGTGTATAAGGGAGCCTGAC
|
ccdB_R_BsaI-SspDnaE-N | TTAAAATTTCGGTACCAAAACTGAGGCAGCTAGTGAGACCCGCGTGGATCCGGCTTAC
|
SspDnaE-N_F_ccdB-BsaI | GGTCTCACTAGCTGCCTCAGTTTTGGTACCG
|
SspDnaE-N_R_taataat-pSB1C3 | GCGGCCGCTACTAGTATTATTATTTAATTGTACCTGCGTCAAGTAATG
|
SspDnaE-C_R_6His-BsaI-ccdB | GCCAGTGGTCTCGAATTGATGGTGATGGTGATGGTGTTTGTTAAAACAGTTGGCGGCGATAG
|
ccdB_F_SspDnaE-C-6His-BsaI | CAAACACCATCACCATCACCATCAATTCGAGACCACTGGCTGTGTATAAGGGAGCCTGAC
|
pSB1C3_F_NpuDnaE-N | CGGGTTGATAATTTGCCGAATTAATAATACTAGTAGCGGCCGCTG
|
pSB1C3_R_FLAG-NpuDnaE-C | CTTGTCGTCATCGTCTTTGTAGTCCATCTAGTACTTTCCTGTGTGACTCTAGAAGC
|
NpuDnaE-C_F_pSB1C3-FLAG | AGGAAAGTACTAGATGGACTACAAAGACGATGACGACAAGCATATGATCAAAATAGCCACACG
|
NpuDnaE-C_R_BsaI-ccdB | GCTCCCTTATACACAGCCAGTGGTCTCGAATTGATTGAAACAATTAGAAGCTATGAAGCC
|
ccdB_F_NpuDnaE-C-BsaI | TTTCAATCAATTCGAGACCACTGGCTGTGTATAAGGGAGCC
|
ccdB_R_BasI-NpuDnaE-N | CTTAAACAGCTAGTGAGACCCGCGTGGATCCGGCTTAC
|
NpuDnaE-N_F_BsaI-ccdB | TAGTAAGCCGGATCCACGCGGGTCTCACTAGCTGTTTAAGCTATGAAACGGAAATATTG
|
NpuDnaE-N_R_pSB1C3 | CCGCTACTAGTATTATTAATTCGGCAAATTATCAACCC
|
NpuDnaE-C_R_6His-BsaI-ccdB | TGGTCTCGAATTGATGATGGTGATGGTGATGATTGAAACAATTAGAAGCTATGAAGCC
|
ccdB_F_NpuDnaE-C-6His-BsaI | TGTTTCAATCATCACCATCACCATCATCAATTCGAGACCACTGGCTGTGTATAAGGGAGCC
|
BBa_E0040_F_EcoR1 | GAAAGGAATTCATGCGTAAAGGAGAAGAACTTTTCACTGGAG
|
BBa_E0040_R_Spe1 | TAGTAACTAGTTTTGTATAGTTCATCCATGCCATGTGTAATCC
|
DNMT1_P1_R_deg.seq.731-1602 | GCATCCAGRTTGCTSCC
|
DNMT1_P2_R_deg.seq.731-1602 | CCACCACACAGCATYTC
|
DNMT1_P3_F_both_seq.731-1602 | ATGTGGGACCCGGCAGC
|
DNMT1_P4_F_deg.seq.731-1602 | CAGTAYACCTTTCATGATGTG
|
LOV-I539E_mut_F | TTTTGGTCTCAGAGGCCGCCGCCAAGCGGGTGAAGCTGGAC
|
LOV-I539E_mut_R | ttttggtctcacctcattttctgcagttttcttaatcagcatg
|
LOV-C450A_mut_F | ttttggtctcagccaggtttctacaaggtcctgaaactgatc
|
LOV-C450A_mut_R | aaaaggtctcttggcgtttcttcccaaaatttcttcac
|
T7RBS BioBrick_F | AAACTACGCTTTAGTAGCTTAATAACTCTGATAGTGCTAGTGTAGATCTC
|
T7RBS BioBrick_R | PLACEHOLDER
|
T7RBS-IGEMHD_F | PLACEHOLDER
|
T7RBS-IGEMHD_R | PLACEHOLDER
|
pSBX-T7fwd | AATTTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCGAATTCGCGGCCGCTT
|
pSBX-T7rev | CTAGAAGCGGCCGCGAATTCGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTA
|
T7RBS-IGEMHD_F_v2 | PLACEHOLDER
|
T7RBS-IGEMHD_R_v2 | PLACEHOLDER
|
T7RBS BioBrick_F_v2 | AATTCGCGGCCGCTTCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATA
|
T7RBS BioBrick_R_v2 | CTAGTATCTCCTTCTTAAAGTTAAACAAAATTATTCTCTAGAAGCGGCCGCG
|
Bba_E0040_F_BsaI=EcoR1 | GAAAGGGTCTCGAATTCATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTC
|
Bba_E0040_R_His6_BsaI=Spe1 | TAGTAGGTCTCACTAGTGTGGTGATGGTGATGATGTTTGTATAGTTCATCCATGCCATGTG
|
BsaI=EcoRI_null_BsaI=Spe1 | GCAGCAGGTCTCAAATTATGCGACTAGAGAGACCGCTGCT
|
BsaI=SpeI_null_BsaI=EcoRI | AGCAGCGGTCTCTCTAGTCGCATAATTTGAGACCTGCTGC
|
1F-smt3_pSB1A3 | AATTCGCGGCCGCTTCTAGATGTCGGACTCAGAAGTCAATCAAGAAGC
|
2R-smt3_Tev_BsaI_ccdb | TCTCTACCCTGGAAGTACAGGTTTTCGGATCCACCAATCTGTTCTCTGTGAGC
|
2F-ccdb_F_smt3_Tev_BsaI | AGATTGGTGGATCCGAAAACCTGTACTTCCAGGGTAGAGACCACTGGCTGTGTATAAGGGAGCC
|
3R-ccdb_R-sortase-his6 | GCCGCTACTAGTATTATTAGTGATGGTGATGGTGATGACCACCGGTTTCCGGAAGTGAGACCCGCGTGGATCCGGCTTACTAAAAGCC
|
3F-pSB1A3_F_ccdb-sortase-his6 | GGTCATCACCATCACCATCACTAATAATACTAGTAGCGGCCGCTGCAGTCCG
|
1R-pSB1A3_smt3 | TTCTTGATTGACTTCTGAGTCCGACATCTAGAAGCGGCCGCGAATTCC
|
ccdB_F_BsmBI | AGAGACGACTGGCTGTGTATAAGGGAGCCTGAC
|
ccdB_R_BsmBI | TGAGACGCGCGTGGATCCGGCTTACTAAAAG
|
mRFP_F_BsaI | AGAGACCCAATACGCAAACCGCCTCTC
|
mRFP_R_BsaI | TGAGACCTATAAACGCAGAAAGGCCCACC
|
T7RBS-IGEMHDv3_F | AATTCGCGGCCGCTTCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATACTAGATGATCGGTGAAATGCACGACTGATAGTA
|
T7RBS-IGEMHDv3_R | CTAGTACTATCAGTCGTGCATTTCACCGATCATCTAGTATCTCCTTCTTAAAGTTAAACAAAATTATTCTCTAGAAGCGGCCGCG
|
NpuDnaEN_F | GTGGGCCTTTCTGCGTTTATAGGTCTCATGCTTAAGCTATGAAACGGAAATATTGACAG
|
NpuDnaEN_R | GCTCCCTTATACACAGCCAGTCGTCTCTACCACCATTCGGCAAATTATCAACCCGCATC
|
NpuDnaEC_F | GCCGGATCCACGCGCGTCTCAGGTGGAATCAAAATAGCCACACGTAAATATTTAGGCAAAC
|
NpuDnaEC_R | GAGAGGCGGTTTGCGTATTGGGTCTCTGTTGGAAGCTATGAAGCCATTTTTGAGTGC
|
SspDnaEN_F | PLACEHOLDER
|
SspDnaEN_R | PLACEHOLDER
|
SspDnaEC_F | PLACEHOLDER
|
SspDnaEC_R | PLACEHOLDER
|
AssemBIyIntN-linker_F | TGGTTAGTAGGGTGGC
|
AssemBIyIntN-linker_R | ATTAGCCACCCTACTA
|
AssemBIyIntC-linker_F | GATGGGTGGCGGTGGC
|
AssemBIyIntC-linker_R | CACCGCCACCGCCACC
|
SB-prep-3P-1 | gccgctgcagtccggcaaaaaa
|
SB-prep-2Ea | atgaattccagaaatcatccttagcg
|
pSBX_F_ccdB-BsmBI | GCCGGATCCACGCGCGTCTCATAATAAATCGGTGAAATGCACGACTGATAG
|
pSBX_R_BsmBI-ccdB | GCTCCCTTATACACAGCCAGTCGTCTCTCATCTAGTATCTCCTTCTTAAAGTTAAACAAAATTATTCTC
|
pSBX_F_mRFP-BsaI | GTGGGCCTTTCTGCGTTTATAGGTCTCATAATAAATCGGTGAAATGCACGACTGATAG
|
pSBX_R_BsaI-mRFP | GAGAGGCGGTTTGCGTATTGGGTCTCTCATCTAGTATCTCCTTCTTAAAGTTAAACAAAATTATTCTC
|
pSBX_F_taataa | TAATAAATCGGTGAAATGCACGACTGATAG
|
pSBX_R_atg | CATCTAGTATCTCCTTCTTAAAGTTAAACAAAATTATTCTC
|
GFP_F_pSBX | TCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATACTAGATGATGCGTAAAGGAGAAGAACTTTTC
|
GFP_R_pSBX | CTAGTACTATCAGTCGTGCATTTCACCGATTTATTATTTGTATAGTTCATCCATGCCATG
|
Lambda-Lys_F_pSBX | TCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATACTAGATGGTAGAAATCAATAATCAACGTAAGGC
|
Lambda-Lys_R_pSBX | CTAGTACTATCAGTCGTGCATTTCACCGATTTATTATACATCAATCTCTCTGACCGTTCCG
|
1F-ccdb_F_pSB1A3-bsaI | ATTTCTGGAATTCGCGGCCGCTTCTAGATGTGAGACCACTGGCTGTGTATAAGGGAGCC
|
1R-pSB1A3_R_bsaI-sortase-his-pSB1A3 | TTATACACAGCCAGTGGTCTCACATCTAGAAGCGGCCGCGAATTCC
|
SspDnaX-S11C_F | TTAGTAAGCCGGATCCACGCGCGTCTCAGGTGGTGGCCTGCTGGTACACAACAGAGACCCAATACGCAAACCGCCTCTCCCC
|
SspDnaX-S11C_R | GGGGAGAGGCGGTTTGCGTATTGGGTCTCTGTTGTGTACCAGCAGGCCACCACCTGAGACGCGCGTGGATCCGGCTTACTAA
|
SspGyrB-S11C_F | TTAGTAAGCCGGATCCACGCGCGTCTCAGGTGGTGGTGTTTTCGTTCACAACAGAGACCCAATACGCAAACCGCCTCTCCCC
|
SspGyrB-S11C_R | GGGGAGAGGCGGTTTGCGTATTGGGTCTCTGTTGTGAACGAAAACACCACCACCTGAGACGCGCGTGGATCCGGCTTACTAA
|
TerDnaE3-S11C_F | TTAGTAAGCCGGATCCACGCGCGTCTCAGGTGGTGGTCTGATCGCGAGCAACAGAGACCCAATACGCAAACCGCCTCTCCCC
|
TerDnaE3-S11C_R | GGGGAGAGGCGGTTTGCGTATTGGGTCTCTGTTGCTCGCGATCAGACCACCACCTGAGACGCGCGTGGATCCGGCTTACTAA
|
NpuDnaEN_R_taataa-pSBX | CTAGTACTATCAGTCGTGCATTTCACCGATTTATTAATTCGGCAAATTATCAACCCGCATC
|
NpuDnaEC_F_pSB-T7RBS | TCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATACTAGATGATCAAAATAGCCACACGTAAATATTTAGGC
|
AssemBIyIntN-linker+H6_F | TGGTGGTCATCATCACCATCACCATTAG
|
AssemBIyIntN-linker+H6_R | ATTACTAATGGTGATGGTGATGATGACC
|
AssemBIyIntC-linker+H6_F | GATGCATCATCACCATCACCACGGT
|
AssemBIyIntC-linker+H6_R | CACCACCGTGGTGATGGTGATGATG
|
GFPn_1-157_R_KGG_TGCT_BsaI | CGTAGGTCTCGAGCACTTACCTCCTTTGTCTGCCATGATGTATACATTG
|
GFPn_1-64_R_KGG_TGCT_BsaI | CGTAGGTCTCAAGCACTTACCTCCGAAAGTAGTGACAAGTGTTGGCC
|
GFPn_1-64_1-157_F_H6_GATG_BsaI | GCATGGTCTCAGATGCATCATCACCATCACCACCGTAAAGGAGAAGAACTTTTC
|
GFPc_158-238_F_NWC_CAAC_BsaI | GCATGGTCTCACAACTGCTGGAACCAAAAGAATGGAATCAAAGTTAAC
|
GFPc_65-238_F_NWC_CAAC_BsaI | GCATGGTCTCACAACTGCTGGAACGGTTATGGTGTTCAATGCTTTGC
|
GFPc_65-238_158-238_R_H6_TAAT_BsaI | CGTAGGTCTCTATTAGTGGTGATGGTGATGATGTTTGTATAGTTCATCCATGCCATGTG
|
mCherryN_1-168_R_KGG_TGCT_BsaI | CGTAGGTCTCAAGCACTTACCTCCCTGCTTGATCTCGCCCTTC
|
mCherryN_1-168_F_H6_GATG_BsaI | GCATGGTCTCAGATGCATCATCACCATCACCACGTGAGCAAGGGCG
|
mCherryC_169-236_R_H6_TAAT_BsaI | CGTAGGTCTCTATTAGTGGTGATGGTGATGATGCTTGTACAGCTCGTCCATGC
|
mCherryC_169-236_F_NWC_CAAC_BsaI | GCATGGTCTCACAACTGCTGGAACAGGCTGAAGCTGAAGGACG
|
mRFPn_1-168_R_KGG_TGCT_BsaI | CGTAGGTCTCAAGCACTTACCTCCTTTCAGTTTCAGACGCATTTTGATTTC
|
mRFPn_1-154_R_KGG_TGCT_BsaI | CGTAGGTCTCAAGCACTTACCTCCGTCTTCCGGGTACATACGTTCG
|
mRFPn_1-154_1-168_F_H6_GATG_BsaI | GCATGGTCTCAGATGCATCATCACCATCACCACGCTTCCTCCGAAGACG
|
mRFPc_169-225_F_NWC_CAAC_BsaI | GCATGGTCTCACAACTGCTGGAACGACGGTGGTCACTACGACG
|
mRFPc_155-225_F_NWC_CAAC_BsaI | GCATGGTCTCACAACTGCTGGAACGGTGCTCTGAAAGGTGAAATC
|
mRFPc_155-225_169-225_R_H6_TAAT_BsaI | CGTAGGTCTCTATTAGTGGTGATGGTGATGATGAGCACCGGTGGAGTG
|
RenLucN_1-229_R_KGG_TGCT_BsaI | CGTAGGTCTCAAGCACTTACCTCCCTTAACGAGAGG
|
RenLucN_1-229_F_H6_GATG_BsaI | GCATGGTCTCAGATGCATCATCACCATCACCACGCTTCCAAGGTGTACG
|
RenLucC_230-311_R_H6_TAAT_BsaI | CGTAGGTCTCTATTAGTGGTGATGGTGATGATGTTACTGCTCGTTCTTCAGCACG
|
RenLucC_230-311_F_NWC_CAAC_BsaI | GCATGGTCTCACAACTGCTGGAACAAGCCCGACGTCGTCC
|
FireLucN_1-437_R_KGG_TGCT_BsaI | CGTAGGTCTCAAGCACTTACCTCCGCGGTCAACGATGAAG
|
FireLucN_1-437_F_H6_GATG_BsaI | GCATGGTCTCAGATGCATCATCACCATCACCACGAAGACGCCAAAAAC
|
FireLucC_438-550_R_H6_TAAT_BsaI | CGTAGGTCTCTATTAGTGGTGATGGTGATGATGCACGGCGATCTTTCC
|
FireLucC_438-550_F_F_NWC_CAAC_BsaI | GCATGGTCTCACAACTGCTGGAACCTGAAGTCTCTGATTAAGTACAAAGGC
|
PCA(t7rbs-npuc-mrfp-npun-igemhd)_F_prefix | CCCCGAATTCGCGGCCGCTTCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATACTAGATG
|
PCA(t7rbs-npuc-mrfp-npun-igemhd)_R_SpeI | CGCTACTAGTACTATCAGTCGTGCATTTCACCG
|
LambdaLys_F_BsaI_c-ext | CTGGTCTCACAACTGCTGGGAAGTAGAAATCAATAATCAACGTAAGGCGTTCC
|
LambdaLys_R_HISTAG_n-ext_BsaI | CTGGTCTCTAGCATTTACCACGGCCGCTGTGATGATGATGATGATGGCTGCCTACATCAATCTCTCTGACCGTTCCG
|
LambdaLys_R_RIGIDLINKER_n-ext_BsaI | CTGGTCTCTAGCATTTACCACGCGGTGCTGCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGCGCCACCTACATCAATCTCTCTGACCGTTCCG
|
LambdyLys_R_FLEXLINKER_n-ext_BsaI | CTGGTCTCTAGCATTTACCACGTCCAGAACCACCACCAGAACCACCTACATCAATCTCTCTGACCGTTCCG
|
Xyla_BB_F_RBS34_X | GCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTAGCACAGACTACTGGCAAAATTG
|
Xyla_BB_R_SP | GGAAGCCTGCAGCGGCCGCTACTAGTATTATTACCACACTGTTACGTTAGAACTTCCAC
|
Xyla_F_BsaI_C-ext | CTGGTCTCACAACTGCTGGGAAGCTAGCACAGACTACTGGCAAAATTG
|
Xyla_F_BsaI_Destroyed-C-ext_His | CTGGTCTCACAACGGCTGGGAAGCTAGCACAGACTACTGGCAAAATTG
|
Xyla_R_BsaI_N-ext | CTGGTCTCTAGCATTTACCACGCCACACTGTTACGTTAGAACTTCCAC
|
Xyla_R_BsaI_N-ext_His | CTGGTCTCTAGCATTTACCACGATGATGGTGATGGTGATGCCACACTGTTACGTTAGAACTTCCAC
|
2GateNinsert_F_X-T7RBS | CCGCTTCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATACTAGATGAGAGACCCAATACGCAAACC
|
2GateNinsert_R_SP | CCGGACTGCAGCGGCCGCTACTAGTATTTATTATGAGACGCGCGTGGATC
|
2GateCinsert_F_X-T7RBS | CGCTTCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATACTAGATGAGAGACGACTGGCTGTGTATAAGG
|
2GateCinsert_R_SP | CCGGACTGCAGCGGCCGCTACTAGTATTATTATGAGACCTATAAACGCAGAAAGG
|
GFP^C_F_ | TATGGTGTTCAATGCTTTGCGAG
|
GFP^C_R_H6 | GTGGTGATGGTGATGATGTTTGTATAGTTCATCCATGCCATGTG
|
NpuDnaE^C_F_T7RBS-H6 | GGAGATACTAGATGCATCATCACCATCACCACATCAAAATAGCCACACGTAAATATTTAGGC
|
NpuDnaE^C_R_GFP^C | CTCGCAAAGCATTGAACACCATAACAATTAGAAGCTATGAAGCCATTTTTGAGTG
|
pSB_F_GFP^C-H6 | AACTATACAAACATCATCACCATCACCACTAATAATACTAGTAGCGGCCGCTGC
|
pSB-T7RBS_R_H6 | GATGGTGATGATGCATCTAGTATCTCCTTCTTAAAGTTAAACAAAATTATTC
|
GFP^N_F_T7RBS-H6 | GGAGATACTAGATGCATCATCACCATCACCACCGTAAAGGAGAAGAACTTTTCACTGGA
|
GFP^N_R_NpuDnaE^N | CAAAGTAGTGACAAGTGTTGGCCATG
|
NpuDnaE^N_F_GFP^N | TGGCCAACACTTGTCACTACTTTGTGTTTAAGCTATGAAACGGAAATATTGAC
|
NpuDnaE^N_R_H6 | GGTGATGATGATTCGGCAAATTATCAACCCGCATC
|
pSB_F_NpuDnaE^N-H6 | GATAATTTGCCGAATCATCATCACCATCACCACTAATAATACTAGTAGCGGCCGCTGC
|
NpuDnaE^C_R_SN->AG-sfGFP^C | GCAAAGCACTGAACACCATAGCAACCAGCAGCTATGAAGCCATTTTTGAGTGC
|
NpuDnaE^C_F_H6 | CATCATCATATCAAAATAGCCACACGTAAATATTTAGGC
|
NpuDnaE^C_R_sfGFP | GCAAAGCACTGAACACCATAGCAATTAGAAGCTATGAAGCCATTTTTGAGTG
|
sfGFP^C_F_NpuDnaE^C | GCTATGGTGTTCAGTGCTTTGCTC
|
sfGFP^C_R_H6 | GTGGTGGTGGTGGTGTTTGTACAGTTCATCCATACCATGC
|
pSB_F_H6-sfGFP^C | GTACAAACACCACCACCACCACCACTAATAATACTAGTAGCGGCCGCTGC
|
pSB-T7RBS_R_NpuDnaE^C-H6 | CGTGTGGCTATTTTGATATGATGATGATGATGATGCATCTAGTATCTCCTTCTTAAAGTTAAACAAAATTATTC
|
NpuDnaE^N_F_sfGFP^N | GACGCTGTGTTTAAGCTATGAAACGGAAATATTGACAGTAG
|
NpuDnaE^N_R_H6 | GATGATTCGGCAAATTATCAACCCGCATC
|
sfGFP^N_F_T7RBS-H6 | GAGATACTAGATGCACCACCACCACCACCACCGTAAAGGCGAAGAGCTGTTC
|
sfGFP^N_R_NpuDnaE^N | CGTTTCATAGCTTAAACACAGCGTCGTTACCAGAGTCG
|
pSB_F_NpuDnaE^N-H6 | GGGTTGATAATTTGCCGAATCATCATCATCATCATCATTAATAATACTAGTAGCGGCCGCTGCAG
|
pSB-T7RBS_R_H6 | GTGGTGGTGGTGCATCTAGTATCTCCTTCTTAAAGTTAAACAAAATTATTC
|
sfGFP^N_R_C->G-NpuDnaE^N | CATAGCTTAAACCCAGCGTCGTTACCAGAGTCG
|
NpuDnaE^N_F_sfGFP-C>-G | CGACGCTGGGTTTAAGCTATGAAACGGAAATATTGACAGTAG
|
pSBX1C3-Ter_F_KpnI | CTAGGGTCTACGTGATCGAGGTACCTACTAGAGCCAGGCATCAAATAAAA
|
pSBX1C3-GFP_R_Ext_IntN_BamHI | GATCGAGGATCCCGCCAGAGAGATCAGAGAGTCACCAGAGATGCAACCAGATTCACGCAGTTTGTATAGTTCATCCATGCCATG
|
BamHI-MTS_bs_minD-KpnI-**_F | GATCCCCTTTACAGGTGCTTGAAGAGCAAAACAAAGGAATGATGGCTAAGATTAAGTCATTTTTCGGAGTAAGATCTTAATAAGGTAC
|
BamHI-MTS_bs_minD-KpnI-**_R | CTTATTAAGATCTTACTCCGAAAAATGACTTAATCTTAGCCATCATTCCTTTGTTTTGCTCTTCAAGCACCTGTAAAGGG
|
SspDnaBC_F_M+H6 | GCCGCTTCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATACTAGATGCATCATCACCATCACCACAGCACAGGAAAAAGAGTTTCTATTAAAGATT
|
SspDnaBC_R_N->A+SGG | GCCGGACTGCAGCGGCCGCTACTAGTATTATTACCCGCCTGAGGCATGGACAATGATGTCATTGGC
|
SspDnaBC_F_M-noH6 | GCCGCTTCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATACTAGATGAGCACAGGAAAAAGAGTTTCTATTAAAGATT
|
CPEC-GFP_F_MTS | GCAAAACAAAGGAATGATGGCTAAGATTAAGTCATTTTTCGGAGTAAGATCTAATACTAGAGCCAGGCATCAAATAAAACGAAAG
|
CPEC-GFP_R_Next_Nint | GCCAGAGAGATCAGAGAGTCACCAGAGATGCAACCAGATTCACGCAGTTATTATTTGTATAGTTCATCCATGCCATGTGTAATC
|
CPEC_GFP_Insert_oligo_F | CTCTGGTGACTCTCTGATCTCTCTGGCGCCTTTACAGGTGCTTGAAGAGCAAAACAAAGGAATGATGGCTAAGATTAAGT
|
dnmt1insert_F_BsaI | ATGTTCGGTCTCAGGGTGGCGGTGGCAAAGATCGCATTAGCTGGCTGGGTCAG
|
dnmt1insert_G_BsaI | ATGTTCGGTCTCATGCGCTGCTCAGCAGACACAGTTTAATTTC
|
mRFP_F_BsmBIBsaI | ACTTACGGTCTCACGCAAGAGACGCAATACGCAAACCGCCTCTC
|
mRFP_R_BsmBIBsaI | GCTTACGGTCTCAGAAGAGAGACGTATAAACGCAGAAAGGCCCACC
|
LambdaLys_R_BsaI | CTGGTCTCTTACATCAATCTCTCTGACCGTTCCG
|
ccdB_F_BsmBI-BsaI | CTCGTCTCATGTAAGAGACCACTGGCTGTGTATAAGGGAGCCTGAC
|
ccdB_R_BsaI-BsmBI | CTCGTCTCAAGCAAGAGACCCGCGTGGATCCGGCTTACTAAAAG
|
CPEC_GFP_Insert_oligo_R | ACTTAATCTTAGCCATCATTCCTTTGTTTTGCTCTTCAAGCACCTGTAAAGGCGCCAGAGAGATCAGAGAGTCACCAGAG
|
Smt3_F_p2Zv2Npu_flag | GATGGACTACAAAGACGATGACGACAAGTCGGACTCAGAAGTCAATCAAGAAGC
|
Smt3_R_p2Zv2Npu | GGCTATTTTGATGGATCCACCAATCTGTTCTCTGTGAG
|
p2Zv2Npu_F_Smt3 | GGTGGATCCATCAAAATAGCCACACGTAAATATTTAGGC
|
p2Zv2Npu_R_flag_Smt3 | CTTGTCGTCATCGTCTTTGTAGTCCATCTAGTATCTCCTTCTTAAAGTTAAACAAAATTATTCTC
|
h6-linker_F | TGTAGGCAGCCATCATCATCATCATCACAGCGGCCGTGGTAAA
|
h6-linker_R | AGCATTTACCACGGCCGCTGTGATGATGATGATGATGGCTGCC
|
flex-linker_F | TGTAGGTGGTTCTGGTGGTGGTTCTGGACGTGGTAAA
|
flex-linker_R | AGCATTTACCACGTCCAGAACCACCACCAGAACCACC
|
rigid-linker_F | TGTAGGTGGCGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGCAGCACCGCGTGGTAAA
|
rigid-linker_R | AGCATTTACCACGCGGTGCTGCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGCGCCACC
|
ccdB-pSBX4C5-mRFP(N)_F | AGAGACGACTGGCTGTGTATAAGGG
|
ccdB-pSBX4C5-mRFP-(N)_R | TGAGACCTATAAACGCAGAAAGGC
|
mRFP-pSBX4C5-ccdB-(C)_F | AGAGACCCAATACGCAAACCG
|
mRFP-pSBX4C5-ccdB-(C)_R | TGAGACGCGCGTGGATC
|
NpuDnaEN_F_C->G-BsaI-mRFP | GGCCTTTCTGCGTTTATAGGTCTCAGGCTTAAGCTATGAAACGGAAATATTGACA
|
NpuDnaEN_R BsmBI_ccdB | CCCTTATACACAGCCAGTCGTCTCTACCACCATTCGGCAAATTATCAACCCG
|
Npu DnaEC_F_BsmBI_ccdB | GATCCACGCGCGTCTCAGGTGGAATCAAAATAGCCACACGTAAATATTTAGG
|
NpuDnaEC_R SN->AG-BsaI-mRFP | CGGTTTGCGTATTGGGTCTCTGCCTGCAGCTATGAAGCCATTTTTGAGTG
|
Universal-S11N_F_BsaI_mRFP | GGCCTTTCTGCGTTTATAGGTCTC
|
Universal-S11N_R BsmBI_ccdB | CCCTTATACACAGCCAGTCGTC
|
Ssp DnaX-S11N_F_C->G_BsaI_mRFP | GGCCTTTCTGCGTTTATAGGTCTCAGGCTTAACCGGCGACAGCC
|
Ssp DnaX-S11C_F_BsmBI_ccdB | GATCCACGCGCGTCTCAGGTGGTGGCCTGCTGGTA
|
Ssp DnaX-S11C_R_BsaI_mRFP | CGGTTTGCGTATTGGGTCTCTGTTGTGTACCAGCAGGCCACCACC
|
Ssp DnaX-S11C_R | CGGTTTGCGTATTGGGTCTCTGCCTGCTACCAGCAGGCCACCACC
|
Ssp GyrB-S11N_F_C->G_BsaI_mRFP | GGCCTTTCTGCGTTTATAGGTCTCAGGCTTCAGCGGTGACACCCTG
|
Ssp GyrB-S11C_F_BsmBI_ccdB | GATCCACGCGCGTCTCAGGTGGTGGTGTTTTCGTT
|
Ssp GyrB-S11C_R_BsaI_mRFP | CGGTTTGCGTATTGGGTCTCTGTTGTGAACGAAAACACCACCACCTGAG
|
Ssp GyrB-S11C_R_BsaI_mRFP | CGGTTTGCGTATTGGGTCTCTGCCTGCAACGAAAACACCACCACCTGAG
|
Ter DnaE3-S11N_F_C->G_BsaI_mRFP | GGCCTTTCTGCGTTTATAGGTCTCAGGCTTAACTTACGAAACTGAGATCATGACC
|
Ter DnaE3-S11C_F_BsmBI_ccdB | GATCCACGCGCGTCTCAGGTGGTGGTCTGATCGCG
|
Ter DnaE3-S11C_R_BsaI_mRFP | CGGTTTGCGTATTGGGTCTCTGTTGCTCGCGATCAGACCACCACC
|
Ter DnaE3-S11C_R | CGGTTTGCGTATTGGGTCTCTGCCTGCCGCGATCAGACCACCACC
|
dnmt1insert_R_Flag_BsaI | GGTCTCAGAAGCTTGTCGTCATCGTCTTTGTAGTCTGCGCTGCTCAGCAGACACAG
|
DNMT1_Substrate_R_1stMedC | GAAGCTGGGACTTCCGGGAGCTTC[5MedC]GATCTGAGAGTGCAA
|
DNMT1_Substrate_F_1stMedC | TTGCACTCTCAGAT[5MedC]GGAAGCTCCCGGGAAGTCCCAGCTTC
|
DNMT1_Substrate_F_2MedC | TTGCACTCTCAGAT[5MedC]GGAAGCTCC[5MedC]GGGAAGTCCCAGCTTC
|
DNMT1_Substrate_F | TTGCACTCTCAGATCGGAAGCTCCCGGGAAGTCCCAGCTTC
|
DNMT1_Substrate_R | GAAGCTGGGACTTCCGGGAGCTTCCGATCTGAGAGTGCAA
|
DNMT1_Substrate_R_2MedC | GAAGCTGGGACTTC[5MedC]GGGAGCTTC[5MedC]GATCTGAGAGTGCAA
|
VF2-C7T | TGCCACTTGACGTCTAAGAA
|
sfGFP_T65C_F | CGACGCTGTGTTATGGTGTTCAGTGCTTTGCTC
|
sfGFP_T65C_R | GAACACCATAACACAGCGTCGTTACCAGAGTCG
|
rfp_F_BsaI | ACTTAACGCAAGAGACCCAATACGCAAACCGCCTCTCC
|
rfp_R_BsaI | ACTTTCGAAGTGAGACCTATAAACGCAGAAAGGCCCACCC
|
LiLaLy_F_BsaI | CTGGTCTCATAATAAATCGGTGAAATGCACGACTG
|
LiLaLy_R_IasB | CTGGTCTCTTACATCAATCTCTCTGACCGTTCCG
|
H6+exteins-insert_F | TGTAGGCAGCCATCATCATCATCATCACAGCGGCCGTGGTAAATGCTGGGAA
|
H6+exteins-insert_R | ATTATTCCCAGCATTTACCACGGCCGCTGTGATGATGATGATGATGGCTGCC
|
XylaLin_F_BsmBI | CTCGTCTCATAATAATACTAGTAGCGGCCGCTG
|
XylaLin_R_IBmsB | CTCGTCTCTCCACACTGTTACGTTAGAACTTCCAC
|
H6insert(Xyla)_F | GTGGGGCAGCCATCACCATCACCATCATAGTGGT
|
H6insert(Xyla)_R | ATTAACCACTATGATGGTGATGGTGATGGCTGCC
|
H6insert+exteins(Xyla)_F | GTGGCATCACCATCACCATCATCGTGGTAAATGCTGGGAA
|
H6insert+exteins(Xyla)_R | ATTATTCCCAGCATTTACCACGATGATGGTGATGGTGATG
|
H6Smt3_F_BBprefix | CGCTTCTAGATGGGCAGCAGCCATCATC
|
mRFPexp_F_H6-TEVc | CACAGCAGCGGCGAAAACCTGTACTTCCAGGGTAGAGACCAATACGCAAACCGCCTCTC
|
mRFPexp_F_Smt3-TEVc-BsaI | GTGGATCCGAAAACCTGTACTTCCAGGGTAGAGACCAATACGCAAACCGCCTCTC
|
mRFPexp_R_BBsuffix-mockSortAc | GCTACTAGTATTATTAGGTTTCCGGAAGTGAGACCTATAAACGCAGAAAGGCCCACC
|
pSB**3_F_mockSortAc | GGTCTCACTTCCGGAAACCTAATAATACTAGTAGCGGCCGCTGCAGTCC
|
pSB**3_R_Smt3 | GCTGCTGCCCATCTAGAAGCGGCCGCGAATTC
|
pSB**3_R_TEVc-H6 | GGTTTTCGCCGCTGCTGTGATGATGATGATGATGACTACTGCCCATCTAGAAGCGGCCGCGAATTC
|
Smt3_R_TEVc-BsaI | CTGGAAGTACAGGTTTTCGGATCCACCAATCTGTTCTCTGTGAG
|
mRFPexp_F_TEVc-BsaI | CAGTAGTGAGAATCTTTACTTCCAGGGTTGAGACCAATACGCAAACCGCCTCTCC
|
mRFPexp_R_BBsuffix-H6-SortAc | CTAGTATTATTAGTGATGGTGATGGTGATGACCACCAGTTTCAGGAAGTGAGACCTATAAACGCAGAAAGGCCCACC
|
pSB**3_R_TEVc-BsaI | CAACCCTGGAAGTAAAGATTCTCACTACTGCCCATCTAGAAGCGGCCGCGAATTCC
|
eGFP_F_BsaI-G5RM | CAGAACGGTCTCAGGGTGGAGGAGGAGGAAGAATGGTGAGCAAGGGCGAGGAGC
|
eGFP_R_BsaI | CAGAACGGTCTCAGAAGCTTGTACAGCTCGTCCATGCC
|
ccdB_F_BsaI-BsmBI | CAGAACGGTCTCACGCATGAGACGACTGGCTGTGTATAAGGGAGCCTGAC
|
ccdB_R_BsaI-BsmBI | CAGAACGGTCTCAGAAGTGAGACGCGCGTGGATCCGGCTTAC
|
DNMT1lnk_0901/1f-FLAG_F | CGCAGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTGATTATAAAGATGATGATGATAAA
|
DNMT1lnk_0901/1f-FLAG_R | CCAGTTTATCATCATCATCTTTATAATCACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACC
|
DNMT1lnk_0825/1r-FLAG-p1F | CGCAGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGAAGCAGCAGCAA
|
DNMT1lnk_0825/1r-FLAG-p1R | TTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGC
|
DNMT1lnk_0825/1r-FLAG-p2F | AAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGCAGCAGCAAGCGGAGCAGCAGCAGAAGCAGCAGCAAAAGATTATAAAGATGATGATGATAAA
|
DNMT1lnk_0825/1r-FLAG-p2R | CCAGTTTATCATCATCATCTTTATAATCTTTTGCTGCTGCTTCTGCTGCTGCTCCGCTTGCTGCTGCTTTTGCTGCTGCTTCTTTTGCTGCTGC
|
EX-BsaI-A-NN-IasB-S_F | AATTCGCGGCCGCTTCTAGAGGTCTCCGATGCCTGCCTGCTAGAGACCTGTCTA
|
EX-BsaI-A-NN-IasB-S_R | CTAGTAGACAGGTCTCTAGCAGGCAGGCATCGGAGACCTCTAGAAGCGGCCGCG
|
EX-BsaI-CC-O-IasB-S_F | AATTCGCGGCCGCTTCTAGAGGTCTCCCAACCCTGCCTAATAGAGACCTGTCTA
|
EX-BsaI-CC-O-IasB-S_R | CTAGTAGACAGGTCTCTATTAGGCAGGGTTGGGAGACCTCTAGAAGCGGCCGCG
|
SsrA-Ctag-BsaI-Cext_BsaI_F | CAACTGCTGGGAAGGAGGAGGTGCTGCTAACGACGAAAACTACGCTCTGGCTGCT
|
SsrA-Ctag-BsaI-Cext_BsaI_R | ATTAAGCAGCCAGAGCGTAGTTTTCGTCGTTAGCAGCACCTCCTCCTTCCCAGCA
|
MTS_bs_minD_C_term_F | CAACTGCTGGGAAGGAGGAGGTCCTTTACAGGTGCTTGAAGAGCAAAACAAAGGAATGATGGCTAAGATTAAGTCATTTTTCGGAGTAAGATCT
|
MTS_bs_minD_C_term_R | ATTAAGATCTTACTCCGAAAAATGACTTAATCTTAGCCATCATTCCTTTGTTTTGCTCTTCAAGCACCTGTAAAGGACCTCCTCCTTCCCAGCA
|
myristoilation-Ntag_F | GATGGGCATCGGCAAGTCCAAGGGCGGAGGCAGGGGCAAG
|
myristoilation-Ntag_R | AGCACTTGCCCCTGCCTCCGCCCTTGGACTTGCCGATGCC
|
NLS-Ntag_F | GATGCCCAAGAAAAAGCGCAAGGTAGGCGGAGGCAGGGGCAAG
|
NLS-Ntag_R | AGCACTTGCCCCTGCCTCCGCCTACCTTGCGCTTTTTCTTGGG
|
OmpA_linker-BsaI_F | GGCCTTGGTCTCAGATGCATATGAAAGCTACTAAACTGGTACTGG
|
OmpA_linker-BsaI_R | GGCCTTGGTCTCGAGCACTTGCCCCTAGAGCTCCCTCCTCCAGAAC
|
pel_B-Ntag_F | GATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCCGGCGGAGGCAGGGGCAAG
|
pel_B-Ntag_R | AGCACTTGCCCCTGCCTCCGCCGGCCATCGCCGGCTGGGCAGCGAGGAGCAGCAGACCAGCAGCAGCGGTCGGCAGCAGGTATTT
|
PKI_NES-Ntag_F | GATGTTGGCTTTGAAATTGGCTGGTTTGGATATTGGCGGAGGCAGGGGCAAG
|
PKI_NES-Ntag_R | AGCACTTGCCCCTGCCTCCGCCAATATCCAAACCAGCCAATTTCAAAGCCAA
|
DNMT1_F_BsaI=CAAC-CWE | ATGTTCGGTCTCACAACTGCTGGGAAAAAGATCGCATTAGCTGGCTGGGTCAG
|
ccdB_R_BsmBI=ATCA-H5SGRGK-TGCT=BsaI | CAGAACGGTCTCAAGCATTTACCACGGCCGCTGTGATGATGATGATGATTGAGACGCGCGTGGATCCGGCTTAC
|
DMMT1_R_H6SGRGT-TGCT=BsaI | CAGAACGGTCTCAAGCAGGTACCACGGCCGCTGTGATGATGATGATGATGTGCGCTGCTCAGCAGACACAG
|
SSpDnaBC_F_ccdB-CN | ATCCACGCGCGTCTCAGGTGGTAGCACAGGAAAAAGAGTTTCTATTAAAG
|
SSpDnaBC_R_CC-mRFP | CGGTTTGCGTATTGGGTCTCTGTTGTGGACAATGATGTCATTGGC
|
SSpDnaBC_R_CC*-mRFP | CGGTTTGCGTATTGGGTCTCTGCCTGCGACAATGATGTCATTGGC
|
SspDnaBN_F_mRFP-NN | GCCTTTCTGCGTTTATAGGTCTCATGCTTATCTGGTGACTCTCTGATCTCTCTGG
|
SspDnaBN_F_mRFP-NN* | GCCTTTCTGCGTTTATAGGTCTCAGGCTTATCTGGTGACTCTCTGATCTCTCTGG
|
SspDnaBN_R_NC-ccdB | CCTTATACACAGCCAGTCGTCTCTACCACCACGGGACGCCAGAGAGATCAGAGAGTCACCAG
|
CBD_His_BsaI_f | ATGTATGGTCTCAGATGCATCATCACCATCACCACGGTGGAGGTACCACAAATCCTGGTGTATC
|
CBD_Nextein_BsaI_r | AAGTTCGGTCTCGAGCCACCAGATTCACGCAGTTGAAGCTGCCACAAGGC
|
DNMT1_F_BsaI=GGGT-G | ATGTTCGGTCTCAGGGTGGTAAAGATCGCATTAGCTGGCTGGGTCAG
|
AsLOV2-mutagenesis_middle_PstI_F | GAAACTGTAGGTTTCTACAAGGTCCTGAAAC
|
AsLOV2_mutagenesis_middle_PstI_R | AGGACCTTGTAGAAACCTACAGTTTCTTCC
|
ccdB_R_BsmBI=ATCA-H5SGRGT-TGCT=BsaI | CAGAACGGTCTCAAGCAGGTACCACGGCCGCTGTGATGATGATGATGATTGAGACGCGCGTGGATCCGGCTTAC
|
DNMT1lnk_0901/1f-FLAG_R_v2 | GAAGTTTATCATCATCATCTTTATAATCACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACC
|
DNMT1lnk_0915/4r-FLAG_p2F | AAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGCAGCAAAAACCGCAGCAGCAGAAGCAGCAGCAAAAGATTATAAAGATGATGATGATAAA
|
DNMT1lnk_0915/3f_p1F | CGCAGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGT
|
DNMT1lnk_0915/3f_p1R | GCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACC
|
DNMT1lnk_0915/3f_p2F | GGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCC
|
DNMT1lnk_0915/3f_p2R | TGATGGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACC
|
rigid+exteins-insert_F | TGTAGGTGGCGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGCAGCACCGCGTGGTAAATGCTGGGAA
|
rigid+exteins-insert_R | ATTATTCCCAGCATTTACCACGCGGTGCTGCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGCGCCACC
|
DNMT1lnk_0915/4r-FLAG_p2R | GAAGTTTATCATCATCATCTTTATAATCTTTTGCTGCTGCTTCTGCTGCTGCGGTTTTTGCTGCTTTTGCTGCTGCTTCTTTTGCTGCTGC
|
DNMT1lnk_0915/2r_p1F | CGCAGCGGAAGCGGCGGCGAAAAACGTGCTGGCGGAAGCGGCGGCGAAAGAAGCGGCGGCGAAAGAAGCGGCGGCGA
|
DNMT1lnk_0915/2r_p1R | TTCTTTCGCCGCCGCTTCTTTCGCCGCCGCTTCTTTCGCCGCCGCTTCCGCCAGCACGTTTTTCGCCGCCGCTTCCGC
|
DNMT1lnk_0915/2r_p2F | AAGAAGCGGCGGCGAAAGAAGCGGCGGCGAAAGCGAACGTGCTGGCGGAAGCGGCGGCGAAAGAAGCGGCGGCGAAAC
|
DNMT1lnk_0915/2r_p2R | TGATGTTTCGCCGCCGCTTCTTTCGCCGCCGCTTCCGCCAGCACGTTCGCTTTCGCCGCCGCTTCTTTCGCCGCCGC
|
DNMT1lnk_0915/6f_p1F | CGCAGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGG
|
DNMT1lnk_0915/6f_p1R | GCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACC
|
DNMT1lnk_0915/6f_p2F | TAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGATTATAAAGATGATGATGATAAA
|
DNMT1lnk_0915/6f_p2R | GAAGTTTATCATCATCATCTTTATAATCGCTACCACCACCGCTACCACCACCGCTACCACCACC
|
DNMT1lnk_0915/1r_p1F | CGCAGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGCAAAC
|
DNMT1lnk_0915/1r_p1R | GCACGTTTGCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGC
|
DNMT1lnk_0915/1r_p2F | GTGCTGGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGAAGCAGCAGCAA
|
DNMT1lnk_0915/1r_p2R | TTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGCCA
|
DNMT1lnk_0915/1r_p3F | AAGAAGCAGCAGCAAAAGCTAACGTGCTGGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAA
|
DNMT1lnk_0915/1r_p3R | GAAGTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGCCAGCACGTTAGCTTTTGCTGCTGC
|
Backbone-H6_R | GATCCGTGGTGGTGGTGGTGG
|
H6-Backbone_F | GGTGGTCATCATCATCATCATCATTAATAATACTAGTAG
|
LOV-6_R_SspDnaXC | GCAGTTGTGTACCAGCAGGCCTTCTTTCGCCGCTTCATCAATATTTTGTGCAGTTTTCTTAATCAGCATGAC
|
LOV-12_R_BsaIIasB | CGAGACCACCTTATTGGTCTCCAATATTTTGTGCAGTTTTCTTAATCAGCATGAC
|
LOV-12_R_SspDnaXC | GCAGTTGTGTACCAGCAGGCCAATATTTTGTGCAGTTTTCTTAATCAGCATGAC
|
sfGFPC_F_BsaIIasB | GGAGACCAATAAGGTGGTCTCGTGCTATGGTGTTCAGTGCTTTGC
|
sfGFPC_F_SspDnaXC | GCCTGCTGGTACACAACTGCTATGGTGTTCAGTGCTTTGC
|
sfGFPC_R_H6 | ATGATGATGATGATGATGACCACCTTTGTACAGTTCATCCATACCATGCG
|
sfGFPN_F_H6 | CACCACCACCACGGATCACGTAAAGGCGAAGAGCTGTTC
|
sfGFPN_R_SspDnaXN | CTGGCTGTCGCCGGTTAAGCACAGCGTCGTTACCAGAG
|
sfGFPN_R_SspDnaXN* | CTGGCTGTCGCCGGTTAAACCCAGCGTCGTTACCAG
|
SspDnaXN_F | TTAACCGGCGACAGCC
|
SspDnaXC*-sfGFP_F_H6 | CACCACCACCACGGATCAGGCCTGCTGGTAGCTGGTTGCTATGGTGTTCAGTGCTTTGC
|
SspDnaXC-sfGFP_F_H6 | CACCACCACCACGGATCAGGCCTGCTGGTACACAACTGCTATGGTGTTCAGTGCTTTGC
|
SspDnaXN_R_H6 | ATGATGATGATGATGATGACCACCGTTTGCAACGAAG
|
mRFP2-168_F_H6 | CACCACCACCACGGATCAGCTTCCTCCGAAGACGTTATC
|
mRFP2-168_R_SspDnaXN | CTGGCTGTCGCCGGTTAAGCATTCGTCGATACCAGATCCTTTCAGTTTCAGACGCATTTTG
|
mRFP2-168_R_SspDnaXN* | CTGGCTGTCGCCGGTTAAACCTTCGTCGATACCAGATCCTTTCAGTTTCAGACGCATTTTG
|
mRFP169-225_F_-6-SspDnaXC-Ext | GCCTGCTGGTACACAACTGCCACACCGACGGTGGTCACTACGACGC
|
mRFP169-225_F_-12-SspDnaXC-Ext-Jalpha | GCCTGCTGGTACACAACTGCCACACCGCGAACCTGGACGGTGGTCACTACGACGC
|
mRFP169-225_F_BsaIIasB | GGAGACCAATAAGGTGGTCTCGGGTGGTCACTACGACGCTGAAG
|
mRFP169-225_F_H6-SspDnaXC | CACCACCACCACGGATCAGGCCTGCTGGTACACAACTGCCACACCGACGGTGGTCACTACGACGC
|
mRFP169-255_F_H6-SspDnaXC* | CACCACCACCACGGATCAGGCCTGCTGGTAGCTGGTTGCCACACCGACGGTGGTCACTACGACGC
|
mRFP169-255_R_H6 | ATGATGATGATGATGATGACCACCAGCACCGGTGGAGTGACG
|
LOV_F_H6 | CACCACCACCACGGATCATTGGCTACTACACTTGAACGTATTG
|
GFPc*158-238_F_NWC_CAAC_BsaI | GCATGGTCTCAAGGCTGCTGGAACCAAAAGAATGGAATCAAAGTTAACTTC
|
GFPc*_R_H6_TAAT=IasB | CGTAGGTCTCTATTAGTGGTGATGGTGATGATGTTTGTATAGTTCATCCATGCCATG
|
GFPn*1-157_R_KGG_GGCT=IasB | CGTAGGTCTCGAGCCCTTACCTCCTTTGTCTGCCATGATGTATACATTG
|
GFPn*_F_H6_GATG_BsaI | GCATGGTCTCAGATGCATCATCACCATCACCACCGTAAAGGAGAAGAACTTTTCACTG
|
mCherryC*169-236_F_BsaI=AGGC-NWC | GCATGGTCTCAAGGCTGCTGGAACAGGCTGAAGCTGAAGGACG
|
mCherryC*_R_H6-TAAT=IasB | CGTAGGTCTCTATTAGTGGTGATGGTGATGATGCTTGTACAGCTCGTCCATGC
|
mCherryN*1-168_R_KGG-GGCT=IasB | CGTAGGTCTCAAGCCCTTACCTCCCTGCTTGATCTCGCCCTT
|
mCherryN*_F_BsaI=GATG-H6 | GCATGGTCTCAGATGCATCATCACCATCACCACGTGAGCAAGGGCGAGG
|
mRFPc*155-225_F_BsaI=AGGC-NWC | GCATGGTCTCAAGGCTGCTGGAACGGTGCTCTGAAAGGTGAAATC
|
mRFPc*169-225_F_BsaI=AGGC-NWC | GCATGGTCTCAAGGCTGCTGGAACGACGGTGGTCACTACGACG
|
mRFPc*_R_H6_TAAT=IasB | CGTAGGTCTCTATTAGTGGTGATGGTGATGATGAGCACCGGTGGAGTG
|
mRFPn*1-154_R_KGG-GGCT=IasB | CGTAGGTCTCAAGCCCTTACCTCCGTCTTCCGGGTACATACGTTC
|
mRFPn*1-168_R_KGG-GGCT=IasB | CGTAGGTCTCAAGCCCTTACCTCCTTTCAGTTTCAGACGCATTTTG
|
mRFPn*_F_H6_BsaI=GATG | GCATGGTCTCAGATGCATCATCACCATCACCACGCTTCCTCCGAAGACGTTATC
|
mRFP_F_BsmBI=GATG=IasB | AATTATCGTCTCAGATGAGAGACCCAATACGCA
|
mRFP_R_BsaI=GGTG=IBmsB | TAATATCGTCTCACACCTGAGACCTATAAACGCAG
|
mRFP_F_BsmBI=TGGT=IasB | AATTATCGTCTCATGGTAGAGACCCAATACGCAA
|
mRFP_R_BsaI=TAAT=IBmsB | TAATATCGTCTCAATTATGAGACCTATAAACGCAGAAAGG
|
bad1_F1 | TGTAGGTGGTGCAGAAGCAGCTGCTAAAGAAGCAGCAGCTAAAGTTAACCTGACCGCTGCTGAAGCT
|
bad1_R1 | TGCTGCAGCTTCAGCAGCGGTCAGGTTAACTTTAGCTGCTGCTTCTTTAGCAGCTGCTTCTGCACCACC
|
bad1_F2 | GCAGCAAAAGAAGCTGCAGCTAAAGTGAACCTGACCGCAGCAGAAGCTGCTGCAAAACGCGGTACC
|
bad1_R2 | AGCAGGTACCGCGTTTTGCAGCAGCTTCTGCTGCGGTCAGGTTCACTTTAGCTGCAGCTTCTTT
|
may1_F1 | TGTAGGTGGTGCAGAAGCTGCTGCTAAAGAAGCTGCTGCAAAAGCAGCAGCTGCACATCCGGAAGCTGCAGAAGCTGC
|
may1_R1 | TTTGCTGCAGCTTCTGCAGCTTCCGGATGTGCAGCTGCTGCTTTTGCAGCAGCTTCTTTAGCAGCAGCTTCTGCACCACC
|
may1_F2 | AGCAAAAGAAGCTGCAGCTAAAGCTAAAACCGCTGCTGAAGCTGCAGCAAAAGAAGCTGCAGCGAAAGCACGCGGTACC
|
may1_R2 | AGCAGGTACCGCGTGCTTTCGCTGCAGCTTCTTTTGCTGCAGCTTCAGCAGCGGTTTTAGCTTTAGCTGCAGCTTCT
|
sgt1_F | TGTAGGTGGTGCAGAAGCAGCTGCTAAAGAAGCTGCTGCAAAACGCGGTACC
|
sgt1_R | AGCAGGTACCGCGTTTTGCAGCAGCTTCTTTAGCAGCTGCTTCTGCACCACC
|
sgt2_F | TGTAGGTGGTGCTGAAGCTGCAGCAAAAGCTGCAGCACATCCGGAAGCTGCAGAAGCTGCAGCTAAACGCGGTACC
|
sgt2_R | AGCAGGTACCGCGTTTAGCTGCAGCTTCTGCAGCTTCCGGATGTGCTGCAGCTTTTGCTGCAGCTTCAGCACCACC
|
sho1_F | TGTAGGTGGTCGCGGTACC
|
sho1_R | AGCAGGTACCGCGACCACC
|
sho2_F | TGTAGGTGGTGCAGAAGCAGCTGCTAAACGCGGTACC
|
sho2_R | AGCAGGTACCGCGTTTAGCAGCTGCTTCTGCACCACC
|
LacI_F_VR | CAGCTCACTCAAAGGCGGTAATACTTTACGGCTAGCTCAGTCCTAGG
|
LacI_R_pSB*C3 | GTTATCCCCTGATTCTGTGGATAACCTTATTACTGCCCGCTTTCCAGTC
|
pSB*C3_F | GGTTATCCACAGAATCAGGGG
|
pSB*C3_R | GTATTACCGCCTTTGAGTGAGC
|
mRFP169-225_F_GSGIDECHT | GATCTGGTATCGACGAATGCCACACCGACGGTGGTCACTACGACGC
|
mRFP169-225_F_GSGIDECHT-Jalpha | GATCTGGTATCGACGAATGCCACACCGCGAACCTGGACGGTGGTCACTACGACGC
|
mRFP2-168_R_GSGIDECHT | GGTGTGGCATTCGTCGATACCAGATCCTTTCAGTTTCAGACGCATTTTG
|
DNMT1lnk_0917/1r_p1F | CGCAGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGCAGTAAATC
|
DNMT1lnk_0917/1r_p1R | GTAAGATTTACTGCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGC
|
DNMT1lnk_0917/1r_p2F | TTACAGCAGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGAAGCAGCAGCAA
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DNMT1lnk_0917/1r_p2R | TTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGCTGCT
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DNMT1lnk_0917/1r_p3F | AAGAAGCAGCAGCAAAAGCTGTAAATCTTACAGCAGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAA
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DNMT1lnk_0917/1r_p3R | GAAGTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGCTGCTGTAAGATTTACAGCTTTTGCTGCTGC
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DNMT1lnk_0917/2r_p1F | CGCAGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGTAAATCTTACAGCAGCAGAAG
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DNMT1lnk_0917/2r_p1R | GCTGCTTCTGCTGCTGTAAGATTTACTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGC
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DNMT1lnk_0917/2r_p2F | CAGCAGCAAAAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGAAGCAGCAGCAAA
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DNMT1lnk_0917/2r_p2R | TTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTTTTGCT
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DNMT1lnk_0917/2r_p3F | AGAAGCAGCAGCAAAAGCAGTAAATCTTACAGCAGCAGAAGCAGCAGCAAAAGCAC
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DNMT1lnk_0917/2r_p3R | TGATGTGCTTTTGCTGCTGCTTCTGCTGCTGTAAGATTTACTGCTTTTGCTGCTGC
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mRFPexp_R_BsmBI=ATCA-H5SGRGT-TGCT=BsaI | CAGAACGGTCTCAAGCAGGTACCACGGCCGCTGTGATGATGATGATGATGGAGACGTATAAACGCAGAAAGGCCCACC
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mut-noPstIT-3A-GateNpuDnaE(C)SN->AG_F | GGCTTCATAGCAGCAGGCAGAGACCCAATAC
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mut-noPstIT-3A-GateNpuDnaE(C)SN->AG_R | CTGCCTGCTGCTATGAAGCCATTTTTGAGTG
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SspDnaE(N)_F_mRFP-NN | GCCTTTCTGCGTTTATAGGTCTCATGCTTAAGTTTTGGTACCGAAATTTTAACC
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SspDnaE(N)_R_NC-ccdB | CCTTATACACAGCCAGTCGTCTCTACCACCACGGGATTTAATTGTACCTGCGTCAAGTAATG
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SSpDnaE(C)_F_ccdB-CN | ATCCACGCGCGTCTCAGGTGGTGTTAAAGTTATCGGTCGTCGATC
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SSpDnaE(C)_R_CC-mRFP | CGGTTTGCGTATTGGGTCTCTGTTGGCGGCGATAGC
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univ#1long_F | TGTAGGTGGTGCAGAAGCAGCAGCTAAAGAAGCT
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univ#1long_R | AGCTGCAGCTTCTTTAGCTGCTGCTTCTGCACCACC
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univ#1short_F | TGTAGGTGGTGCAGAAGCTGCT
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univ#1short_R | CTTTGCAGCAGCTTCTGCACCACC
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univ#3_F | GCTGAAGCAGCTGCTAAAGAAGCAGCAGCTAAAGAAGCTGCTGCTAAAGAAGCTGCAGCTAAAGAAGCAGCTGCT
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univ#3_R | TGCCTTAGCAGCTGCTTCTTTAGCTGCAGCTTCTTTAGCAGCAGCTTCTTTAGCTGCTGCTTCTTTAGCAGCTGC
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bad2#2_F | GCAAAGGTGAACCTGACAGCT
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bad2#2_R | TTCAGCAGCTGTCAGGTTCAC
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bad2#4_F | AAGGCAAAAACCGCAGCTGAAGCTGCTGCAAAAGAAGCTGCAGCTAAAGAAGCTGCTGCAAAAGCTCGTGGTACC
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bad2#4_R | AGCAGGTACCACGAGCTTTTGCAGCAGCTTCTTTAGCTGCAGCTTCTTTTGCAGCAGCTTCAGCTGCGGTTTT
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bad3#2_F | GCAAAGGCATCTCTGCCGGCAGCT
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bad3#2_R | TTCAGCAGCTGCCGGCAGAGATGC
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bad3#4_F | AAGGCAAACGTGCTGGCAGAAGCAGCTGCTAAAGCACGTGGTACC
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bad3#4_R | AGCAGGTACCACGTGCTTTAGCAGCTGCTTCTGCCAGCACGTT
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bad4#2_F | GCAGCTAAAGAAGCTGCTGCTAAAGAAGCAGCTGCTAAAGAAGCAGCAGCTAAAGCTGTGAACCTGACCGCA
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bad4#2_R | TTCAGCTGCGGTCAGGTTCACAGCTTTAGCTGCTGCTTCTTTAGCAGCTGCTTCTTTAGCAGCAGCTTCTTT
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bad4#4_F | AAGGCAGTGAACCTGACCGCAGCTGAAGCTGCAGCTAAAGAAGCAGCTGCTAA
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bad4#4_R | GCTTCTTTAGCAGCTGCTTCTTTAGCTGCAGCTTCAGCTGCGGTCAGGTTCAC
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bad4#5_F | AGAAGCAGCTGCTAAAGAAGCAGCTGCTAAAGAAGCAGCTGCTAAAGCACGTGGTACC
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bad4#5_R | AGCAGGTACCACGTGCTTTAGCAGCTGCTTCTTTAGCAGCTGCTTCTTTAGCAGCT
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may2#2_F | GCAAAGGCTAAAACCGCTGCTGAAGCAGCAGCTAAAGAAGCAGCTGCGAAACGTGGTACC
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may2#2_R | AGCAGGTACCACGTTTCGCAGCTGCTTCTTTAGCTGCTGCTTCAGCAGCGGTTTTAGC
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may3#2_F | GCAGCTAAAGCTACCGGTGATCTGGCTGCAGAAGCAGCTGCAAAAGAAGCTGCTGCAAAAGCTCGCGGTACC
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may3#2_R | AGCAGGTACCGCGAGCTTTTGCAGCAGCTTCTTTTGCAGCTGCTTCTGCAGCCAGATCACCGGTAGCTTT
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ord1#2_F | GCAGCTAAAGCTACCGGTGATCTGGCTGCTGAAGCAGCAGCTAAAGCTGCACGTGGTACC
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ord1#2_R | AGCAGGTACCACGTGCAGCTTTAGCTGCTGCTTCAGCAGCCAGATCACCGGTAGCTTT
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ord2#2_F | GCAAAGGCTGCTGCAGCTGATGGTACCCTGGCAGAAGCTGCTGCTAAAGCAGCTCGTGGTACC
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ord2#2_R | AGCAGGTACCACGAGCTGCTTTAGCAGCAGCTTCTGCCAGGGTACCATCAGCTGCAGCAGC
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ord3#2_F | GCAGCTAAAGCTAGCCTGCCGGCTGCTGCAGAAGCTGCTGCAAAAGAAGCTGCAGCTAAACGTGGTACC
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ord3#2_R | AGCAGGTACCACGTTTAGCTGCAGCTTCTTTTGCAGCAGCTTCTGCAGCAGCCGGCAGGCTAGCTTT
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ord4#2_F | GCAGCTAAAGCTACCGGTGATCTGGCAGCAGAAGCTGCAGCTAAACGTGGTACC
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ord4#2_R | AGCAGGTACCACGTTTAGCTGCAGCTTCTGCTGCCAGATCACCGGTAGCTTT
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ord5#2_F | GCAAAGGCTGCTACCGGTGATCTGGCAGCAGAAGCAGCTGCTAAAGCACGTGGTACC
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ord5#2_R | AGCAGGTACCACGTGCTTTAGCAGCTGCTTCTGCTGCCAGATCACCGGTAGCAGC
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ord6#2_F | GCAAAGGCTAGCCTGCCGGCTGCAGCTGAAGCTGCAGCGAAAGAAGCAGCAGCTAAAGCTCGCGGTACC
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ord6#2_R | AGCAGGTACCGCGAGCTTTAGCTGCTGCTTCTTTCGCTGCAGCTTCAGCTGCAGCCGGCAGGCTAGC
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sgt3#2_F | GCAAAGGTGAACCTGACCGCTGCTGAAGCTGCAGCTAAACGTGGTACC
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sgt3#2_R | AGCAGGTACCACGTTTAGCTGCAGCTTCAGCAGCGGTCAGGTTCAC
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sgt4#2_F | GCAAAGGCTGCAGCACATCCGGAAGCTGCTGAAGCAGCTGCAAAAGCACGTGGTACC
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sgt4#2_R | AGCAGGTACCACGTGCTTTTGCAGCTGCTTCAGCAGCTTCCGGATGTGCTGCAGC
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sho3#2_F | GCAAAGGCTACCGGTGATCTGGCAGAAGCAGCTGCTAAACGTGGTACC
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sho3#2_R | AGCAGGTACCACGTTTAGCAGCTGCTTCTGCCAGATCACCGGTAGC
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rigid_RGT_F | TGTAGGTGGCGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGCAGCACCGCGTGGTACC
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rigid_RGT_R | AGCAGGTACCACGCGGTGCTGCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGCGCCACC
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ccdB_R_BsmBI=ATCA-H5SGRGT-TGCT=BsaI | CAGAACGGTCTCAAGCAGGTACCACGGCCGCTGTGATGATGATGATGATTGAGACGCGCGTGGATCCGGCTTAC
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ccdB_R_BsmBI=ATCA-H5SGRGT-iGEMHD | CGTGCATTTCACCGATTTATTAGGTACCACGGCCGCTGTGATGATGATGATGATTGAGACGCGCGTGGATCCGGCTTAC
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Smt3_F_T7RBS | TTTGTTTAACTTTAAGAAGGAGATACTAGATGTCGGACTCAGAAGTCAATCAAGAAG
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DNMT1_F_Smt3-CWE | GTGGATGCTGGGAAAAAGATCGCATTAGCTGGCTGG
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Smt3_R_CWE-DNMT1 | TCTTTTTCCCAGCATCCACCAATCTGTTCTCTGTGAGC
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DNMT1_R_H6SGRGT-iGEMHD | CGTGCATTTCACCGATTTATTAGGTACCACGGCCGCTGTGATGATGATGATGATGTGCGCTGCTCAGCAGACAC
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DNMT1_R_RFC10suffix | TGCAGCGGCCGCTACTAGTATTATTATGCGCTGCTCAGCAGAC
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DNMT1/BB1_R_DNMT1/BB2 | CATACTGACCTGCTTGCAGAACACCAAAGG
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DNMT1/BB2_F_DNMT1/BB1 | GGTGTTCTGCAAGCAGGTCAGTATGGTGTTGC
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DNMT1/BB2_R_DNMT1/BB3 | CATCATGAAAGGTATATTGCAGTTTATGTGCAATAAC
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DNMT1/BB3_F_DNMT1/BB2 | GCACATAAACTGCAATATACCTTTCATGATGTG
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pSB**3_F_TAATAA | TAATAATACTAGTAGCGGCCGCTGCAGTCC
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Cas9CtrlSplit1_Fw | TGCTTCAACGGCGATAGCCTGCACGAG
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Cas9CtrlSplit1_Re | GTTGAAGCAGGACACCTGGGCTTTCTGG
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Cas9CtrlSplit2_Fw | GCCAGAGAGTACTGCTTCAACCAGACCACCCAGAAGG
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Cas9CtrlSplit2_Re | CTGGTTGAAGCAGTACTCTCTGGCCATTTCGATC
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Cas9Split1C_F_CFN_NpuDnaEC | CAACTGCTTCAACGGCGATAGTCTGCACGAGCAC
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Cas9Split1N_R_NpuDnaEN | CATAGCTCAAGCAGGACACCTGGGCTTTCTGGATG
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Cas9Split2C_F_CF_NpuDnaEC | CCAACTGCTTCAACCAGACCACCCAGAAGGG
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Cas9Split2N_R_Y_NpuDnaEN | CATAGCTCAAGCAGTACTCTCTGGCCATTTCGATC
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mCherry_Fw_BamHI | ATCGCGGATCCGGCACCATGGTGAGCAAGGGCGAGGAG
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mCherry_Rv_HindIII | GCGATAAGCTTCTACTTGTACAGCTCGTCCATGCCGC
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NpuDnaECSplit1&2C_F_NLS_Flag | AGACGATGACGATAAGCCAAAGAAGAAGCGGAAGGTCGGTGGTATCAAAATAGCCACACGTAAATATTTAGGC
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NpuDnaECSplit1C_R_CFN_Cas9Split1C | CTATCGCCGTTGAAGCAGTTGGAAGCTATGAAGCCATTTTTG
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NpuDnaECSplit2C_R_CF_Cas9Split2C | GTGGTCTGGTTGAAGCAGTTGGAAGCTATGAAGCCATTTTTG
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NpuDnaENSplit1&2N_R_NLS | CTCCGTGGATACCGACCTTCCGCTTCTTCTTTGGGGCACCACCATTCGGCAAATTATCAACCC
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NpuDnaENSplit1N_F_Cas9Split1N | CCAGGTGTCCTGCTTGAGCTATGAAACGG
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NpuDnaENSplit2N_Fw_Y_Cas9Split2N | GCCAGAGAGTACTGCTTGAGCTATGAAACGG
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PCDna3.1Split1&2C_R_Flag_NLS | CTTCTTTGGCTTATCGTCATCGTCTTTGTAATCCATGGTGGCGGATCCACTAG
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PCDna3.1Split1N_F_NLS | GAAGGTCGGTATCCACGGAGTCCCAGCAGCCTGAAAGCTTAAGTTTAAACCGCTGATCAGC
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PCDna3.1Split2N_F_NLS | GAAGGTCGGTATCCACGGAGTCCCAGCAGCCTGAAAGCTTAAGTTTAAACCGCTG
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FS_47_Cas9#49535_BamHI_fw | ATCGCGGATCCGCCACCATGGATTAC
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FS_48_Cas9#49535_HindIII_rv | GCGATAAGCTTTTTCTTTTTCTTAGCTTGACCAGC
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LOV-muta-PstI-middle2_F | GTGAAGAAATTTTGGGAAGAAACTGTAGGTTTCTACAAGGTCCTG
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LOV-muta-PstI-middle2_R | CAGTTTCAGGACCTTGTAGAAACCTACAGTTTCTTCCCAAAATTTC
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