Team:Goettingen/protocol Peptide

Overview

PCR Methods

• PfuS PCR
• Fusion PCR
• E.coli colony PCR
• Yeast colony PCR

Plasmid Construction

• Restriction of DNA
• Ligation of DNA fragments
• BP recombination reaction
• LR recombination reaction
• SEAMLESS Cloning
• Peptide Library construction

Plasmid Transformation

• E.coli competent cells
• Plasmid isolation (E.coli)
• E.coil transformation
• Plasmid isolation (Yeast)
• Yeast transformation

Colony Scanning

• E.coli cracking
• Auto-activity Test
• Yeast-Two-Hybrid Assay with Robot
• Yeast-Two-Hybrid Screening

Protein Assessment

• SDS PAGE
• Coomassie Staining
• Protein Expression
• Protein Purification
• Protein Dialysis
• Bradford Assay

In vivo tests

• Production of spore suspension
• Cell cultivation
• Immunofluorescence Staining
• Calcofluor staining