Golden Gate Is10

To do PCR to amplified :
Is10 kan GFP Is10 in the plasmid pNK2 with primer 68 and 69.
OriVR6Kgamma muted with primer 70 and 71
To send the fragment like biobrick.

Golden Gate Universal Transposon Plasmid

To do PCR to build the universal plasmid :
Is10 (1) with primer 74 and 75
Is10 (2) with primer 76 and 77
Ori VR6K gamma with primer 78 and 79
Tn10 (with this promoter and terminator) with primer 80 and 81

Oct 01