Competent cells

A new stock of DH5α and DH5α pir competent is produced.

Golden Gate Tn10

A PCR colonies on white cells DH5α transformed with pSB1C3 Tn10 with primer VF and VR.
The revelation shows bands at 1500 bp, which correspond to Tn10 (1 200 bp) and every side of VF and VR.
The golden Gate is OK and the plasmid is start to send at the registry.

Aug 10