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Team:Evry/Notebook/Transformation/10-06-2014
From 2014.igem.org
New protocols of electrocompetent Pseudovibrio
On a pre-culture of Pseudovibrio in MB 1X 3mL, we relaunch the culture in 100mL of MB 1X. We made the electrocompetent protocol (Protocole).
We tested to make 5 and 6 washes with glycerol 10% instead of three.
Stocks of cells were stored at -80°C (3 weeks maximum)
