Team:Evry/Notebook/Sensing/PCBs/08-26-2014

From 2014.igem.org

Picture

Sensor construction bphR2/PbpR1

We wanted to assemble the construction by using golden gate. After to have received primers, PCR was done to amplify J23114-RBS, bphR2, bphR1-RBS, RFP and terminator with golden gate primers. The tail of each part is:

  • J23114-RBS = 82bp
  • bphR2 mutated = 929bp
  • bphR1-RBS = 340bp
  • RFP = 732bp
  • Terminator = 162bp

Simultaneously, the product of digestion of bphR2 and pSB1C3 has been migrated to verify the digestion then, bphR2 and pSB1C3 has been ligated and we have done a heat shock transformation.

Aug 26

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