Our BioBricks

Degron BioBricks

BBa_K1399000 - RFP(AAV)

Mutant RFP from Discosoma striata (coral) (see Part:BBa_E1010) with added AAV-ssrA degradation tag (Part:BBa_I11012). The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed.

BBa_K1399001 - RFP(LVA)

Mutant RFP from Discosoma striata (coral) (see Part:BBa_E1010) with added LVA-ssrA degradation tag. The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. LVA tag is commonly found attached to repressors in various gene networks (e.g. oscillators). The tag encodes peptide sequence AANDENYALVA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB. ClpA and ClpX then form a proteasome-like complex with ClpP protease and the protein is degraded.

BBa_K1399002 - RFP(LAA)

Mutant RFP from Discosoma striata (coral) (see Part:BBa_E1010) with added LAA-ssrA degradation tag (Part:BBa_M0050). The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes peptide sequence AANDENYALAA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB. ClpA and ClpX then form a proteasome-like complex with ClpP protease and the protein is degraded.

BBa_K1399003 - RFP(DAS)

Mutant RFP from Discosoma striata (coral) (see Part:BBa_E1010) with added SsrA-DAS degradation tag (Part:Ba_M0052). The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes peptide sequence AANDENYADAS and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB. ClpA and ClpX then form a proteasome-like complex with ClpP protease and the protein is degraded.

BBa_K1399004 - GFP(LVA)

GFP (mut3b) (see Part:BBa_E0040) with added LVA-ssrA degradation tag. The tag increases GFP turn-over rate, thus providing better temporal resolution of green fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes peptide sequence AANDENYALVA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB. ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.

BBa_K1399005 - GFP(AAV)

GFP (mut3b) (Part:BBa_E0040) with added AAV-ssrA degradation tag (Part:BBa_I11012). The tag increases GFP turn-over rate, thus providing better temporal resolution of green fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes peptide sequence AANDENYAAAV and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB. ClpA and ClpX then form a proteasome-like complex with ClpP protease and the protein is degraded.

BBa_K1399006 - GFP(LAA)

GFP (mut3b) (Part:BBa_E0040) with added LAA-ssrA degradation tag (see Part:BBa_M0050). The tag increases GFP turn-over rate, thus providing better temporal resolution of green fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes E.coli wild type ssrA peptide sequence AANDENYALAA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB. ClpA and ClpX then form a proteasome-like complex with ClpP protease and the protein is degraded.

BBa_K1399007 - GFP(DAS+2)

GFP (mut3b) (see Part:BBa_E0040) with added engineered NYADAS-ssrA degradation tag (Part:BBa_M0051). The tag increases GFP turn-over rate, thus providing better temporal resolution of green fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. SsrA tags encode peptide sequence that is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.

BBa_K1399008 - GFP(DAS)

GFP (mut3b) (see Part:BBa_E0040) with added DAS-ssrA degradation tag (see Part:BBa_M0052). The tag increases GFP turn-over rate, thus providing better temporal resolution of green fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. SsrA tags encode peptide sequence that is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.

BBa_K1399010 - P_lac-B0034-RFP(AAV)

Lactose/IPTG inducible promoter with RFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged RFP is actively degraded within cell, thus it provides better temporal resolution of red fluorescence and promoter activity.

BBa_K1399011 - P_lac-B0034-RFP(LVA)

Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LVA-ssrA degradation tag and terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. This part can be considered as improved version of Part:BBa_J04451 as it does not contain StuI restriction site coding for additional 'RP' residues between RFP and LVA tag.

BBa_K1399012 - P_lac-B0034-RFP(LAA)

Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LAA-ssrA degradation tag and terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity.

BBa_K1399013 - P_lac-B0034-RFP(DAS)

Lactose/IPTG inducible promoter with RFP reporter tagged with DAS-ssrA degradation tag followed by terminator. The tagged RFP is degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. Presence of SspB is crucial for effective protein degradation.

BBa_K1399014 - P_lac-B0034-GFP(AAV)

Lactose/IPTG inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.

BBa_K1399015 - P_lac-B0034-GFP(LVA)

Lactose/IPTG inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.

This part can be considered as improved version of Part:BBa_J04431 as it does not contain StuI restriction site coding for additional 'RP' residues between GFP and LVA tag.

BBa_K1399016 - P_lac-B0034-GFP(LAA)

Lactose/IPTG inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.

BBa_K1399017 - P_lac-B0034-GFP(DAS+2)

Lactose/IPTG inducible promoter with GFP reporter tagged with DAS+2-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity. Presence of SspB is crucial for effective protein degradation.

BBa_K1399018 - P_lac-B0034-GFP(DAS)

Lactose/IPTG inducible promoter with GFP reporter tagged with DAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity. Presence of SspB is crucial for effective protein degradation.

BBa_K1399019 - P_tet-B0034-GFP(AAV)

Tetracycline/aTc inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.

BBa_K1399020 - P_tet-B0034-GFP(LVA)

Tetracycline/aTc inducible promoter with GFP reporter tagged with LVA-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.

BBa_K1399021 - P_tet-B0034-GFP(LAA)

Tetracycline/aTc inducible promoter with GFP reporter tagged with LAA-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.

BBa_K1399022 - P_tet-B0034-GFP(DAS+2)

Tetracycline/aTc inducible promoter with GFP reporter tagged with NYADAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity. Presence of SspB is crucial for effective protein degradation.

BBa_K1399023 - P_tet-B0034-GFP(DAS)

Tetracycline/aTc inducible promoter with GFP reporter tagged with DAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity. Presence of SspB is crucial for effective protein degradation.

Population Regulation BioBricks

BBa_K1399024 - P_lac-rmf

The rmf gene slows growth by sequestering ribosomes. It is under the control of a lactose inducible promoter.

BBa_K1399025 - P_lac-rsd

The rsd gene slows growth by interfering with sigma factor subunit of RNA polymerase. It is under the control of a lactose inducible promoter.