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Team:ETH Zurich/labblog/20140707
From 2014.igem.org
Contents |
Digest and miniprep
Monday, July 7th
Optimizing Restriction Endonuclease Reactions
Digest of 291 and 561
291: (2x)
1μL AscI
1μL FseI
0.5μL PvuI
0.5μL AseI
20 μL DNA 291
5μL Cut Smart Buffer
fill up with 22 μL H2O to 50μL
Miniprep of 35 and 36
