From 2014.igem.org

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9^th September

• SDS-PAGE
  Resuspension of 1-2mg of lyophilized ELP in cold PBS
  
  We make a range:
  

  ELP	2μl	5μl	10μl
  PBS	8μl	5μl	0μl
  Sample Buffer 2X	10μl	10μl	10μl

  
  
  => Heat 3min at 85°C
  => Coloration with Coomassie Brilliant blue
  


• Preparation of 2 tubes for mass spectrometry
  5mg and 7mg of ELP