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From 2014.igem.org

5^th June

• We obtain three ELPs strains streaked on LB medium
  • A strain of ELP VPGXG-20
    Strain A : pUC19-New-Met20 in NEB 5-alpha F'Iq Competent E. coli
    1. Used for cloning or others
    2. Amp^R
  
  
  • A strain of ELP VPGXG-40
    Strain B : pUC19-New-Met40 in Stbl3 E.coli (The Stbl3 E. coli strain is derived from the HB101 E. coli strain and is recommended for use when cloning unstable inserts such as lentiviral DNA containing direct repeats)
    1. Used for cloning or others
    2. Amp^R
  
  
  • A strain of ELP VPGXG-60
    Strain C : pUC19-GS60 in Stbl3 E.coli
    1. Used for cloning or others
    2. Amp^R


• Inoculation of 3 LB medium for store


• Transformation by electroporation of our bacteria with low copy IGEM backbone pSB6A1(2014 Kit Plate 4, well 2L)

• We use BL21 E.coli for the production


• We obtain three ELP strains streaked on LB medium :
  • A strains of ELP VPGXG-20
    
    Pattern: MW-(VPGXG)₂₀
    Number of amino acids : 102
    Molecular weight : 8685.4 Da
    Theoretical pI : 5.28
    
    Strain A’ : pET44a-New-Met20 in BL21 E.coli
    1. Used for cloning or others
    2. Amp^R
  
  
  • A strain of ELP VPGXG-40
    
    Pattern : MW-(VPGXG)₄₀
    Number of amino acids : 202
    Molecular weight : 17035.4 Da
    Theoretical pI : 5.28
    
    Strain B’ : pET44a-New-Met40 in BL21 E.coli
    1. Used for cloning or others
    2. Amp^R
  
  
  • A strain of ELP VPGXG-60
    
    Pattern : MW-(VPGXG)₆₀
    Number of amino acids : 303
    Molecular weight : 25849.3 Da
    Theoretical pI : 5.27
    
    Strain C’ : pET44a-GS60 in BL21 E.coli
    1. Used for cloning or others
    2. Amp^R