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From 2014.igem.org

17^th June

• Protocol LB + Glucose or Glycerol
  Bactotryptone 5g
  Yeast Extract 5g
  NaCl 5g
  - Glucose 1g/L
  - Glycerol 10% (126g/L)
  1g/l -> 7,9ml, pour 500mL -> 3,96ml
  

• Production ELP in low copy backbone
  OD inoculating at 9h10
  OD600 40 : 0,2 (0,196)
  OD60060 : 0,13
  
  10h10
  OD600 40 : 0,056
  OD600 60 : 0,672
  
  Induction 10h25
  
  OD600 40 : 0,708
  OD600 60 : 0,734
  (11h25) 1h post induction
  OD600 40 =0,736
  OD600 60 = 0,965
  (12h25) 2h post induction
  OD600 40 = 0,928
  OD600 60 = 1,203
  (13h25) 3h post induction
  OD600 40 = 1,170
  OD600 60= 1,357
  (14h25) 4h post induction
  OD600 40 = 0,339
  OD600 60=1,563
  (15h25) 5h post induction
  OD600 40 = 0,598
  OD600 60 = 1,856
  
  

• Purification during the journey