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From 2014.igem.org

28^th July

• SLP gene synthesis
  
  
  
  PCR Parameter:
  92°C 1min
  12 cycles : 92°C 10s, 66°C 30 sec, 72°C 5s
  End at 4°C
  
  
  
  
  PCR Parameter:
  98°C 30 sec
  35 cycles : 98°C 10 sec, 20 sec gradient 68°C delta 4°C ; tube ° 66°C ; tube °°° 70°C, 72°C 30 sec
  Final extension 5 min 72°C
  End at 4°C
  

Please look our planning of production and purification